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MS Mouse IL-1α(Interleukin 1Alpha) ELISA Kit

Citations(11)Uniprot : P01582

Cat.No.:E-MSEL-M0039
Reactivity: Mouse

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MS Series

The sample size of MS series ELISA kit is only 1/4 of that of the traditional sandwich ELISA kit, which can effectively solve the problem of insufficient sample size.

Product Details

Properties

Assay type	Sandwich-ELISA
Format	48T/96T/96
Assay time	3.5h
Detection range	7.81-500 pg/mL
Sensitivity	3.01 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 25μL
Specificity	This kit recognizes Mouse IL-1α in samples. No significant cross-reactivity or interference between Mouse IL-1α and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Mouse IL-1α concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-1α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-1α. You can calculate the concentration of Mouse IL-1α in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (50×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (50×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse IL-1α were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse IL-1α were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	27.19	60.62	212.52	24.70	63.66	198.53
Standard deviation	1.65	2.48	8.88	1.56	3.29	7.72
CV (%)	6.06	4.09	4.18	6.31	5.17	3.89

Recovery

The recovery of Mouse IL-1α spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	94-108	100
EDTA plasma (n=8)	86-101	94
Cell culture media (n=8)	86-97	91

Linearity

Samples were spiked with high concentrations of Mouse IL-1α and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P01582
Synonyms	IL1A, IL1-A, IL1, IL1F1,Preinterleukin 1 Alpha, Hematopoietin-1, Pro-Interleukin-1-Alpha
Research Area	Cancer, Metabolism, Immunology

Assay Procedures

Citations

Bioactive Materials (2021) IF: 14.593

Secretions from hypochlorous acid-treated tumor cells delivered in a melittin hydrogel potentiate cancer immunotherapy

DOI: 10.1016/j.bioactmat.2021.07.019
PMID: 34820587	Sample: B16–F10 cells,Red blood cells

Bone Research (2019) IF: 13.567

Extracellular vesicles from human urine-derived stem cells prevent osteoporosis by transferring CTHRC1 and OPG

DOI: 10.1038/s41413-019-0056-9
	Sample: serum

Bone Research (2018) IF: 12.354

Omentin-1 prevents inflammation-induced osteoporosis by downregulating the pro-inflammatory cytokines

DOI: 10.1038/s41413-018-0012-0
	Sample: Serum

JOURNAL OF NANOBIOTECHNOLOGY (2022) IF: 10.435

Intracellular marriage of bicarbonate and Mn ions as “immune ion reactors” to regulate redox homeostasis and enhanced antitumor immune responses

DOI: 10.1186/s12951-022-01404-x
	Sample: serum

Oxidative Medicine and Cellular Longevity (2023) IF: 7.31

RRP Regulates Autophagy through the AMPK Pathway to Alleviate the Effect of Cell Senescence on Atherosclerosis

DOI: 10.1155/2023/9645789
	Sample: serum

Nutrients (2022) IF: 5.719

A Lard and Soybean Oil Mixture Alleviates Low-Fat–High-Carbohydrate Diet-Induced Nonalcoholic Fatty Liver Disease in Mice

DOI: 10.3390/nu14030560
	Sample: liver

EXPERIMENTAL GERONTOLOGY (2021) IF: 4.032

The elevated level of IL-1α in the bone marrow of aged mice leads to MSC senescence partly by down-regulating Bmi-1

DOI: 10.1016/j.exger.2021.111313
PMID: 33740618	Sample: Bone marrow fluid (BMF)

JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36

Protective effect of Di'ao Xinxuekang capsule against doxorubicin-induced chronic cardiotoxicity

DOI: 10.1016/j.jep.2021.114943
PMID: 34954266	Sample: serum

NEUROBIOLOGY OF AGING (2018) IF: 4.454

CIP2A-promoted astrogliosis induces AD-like synaptic degeneration and cognitive deficits

DOI: 10.1016/j.neurobiolaging.2018.11.023
PMID: 30594047	Sample: Tissue homogenate,Cell culture medium

INTERNATIONAL IMMUNOPHARMACOLOGY (2020) IF: 3.943

10-Hydroxydecanoic acid inhibits LPS-induced inflammation by targeting p53 in microglial cells

DOI: 10.1016/j.intimp.2020.106501
	Sample: Cell culture supernatant

Dose-Response (2020) IF: 2.438

Plasma Proteins as Biomarkers of Mortality After Total Body Irradiation in Mice:

DOI: 10.1177/1559325820920141
	Sample: plasma

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