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MS Mouse OPG(Osteoprotegerin) ELISA Kit

Citations(7)Uniprot : O08712

Cat.No.:E-MSEL-M0038
Reactivity: Mouse

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96T
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96T*5
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MS Series

The sample size of MS series ELISA kit is only 1/4 of that of the traditional sandwich ELISA kit, which can effectively solve the problem of insufficient sample size.

Product Details

Properties

Assay type	Sandwich-ELISA
Format	24T/48T/96
Assay time	3.5h
Detection range	125.00-8000 pg/mL
Sensitivity	62.87 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 25μL
Specificity	This kit recognizes Mouse OPG in samples. No significant cross-reactivity or interference between Mouse OPG and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Mouse OPG concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse OPG. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse OPG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse OPG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse OPG. You can calculate the concentration of Mouse OPG in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (50×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (50×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse OPG were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse OPG were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	437.57	693.40	3412.09	452.19	753.49	3342.64
Standard deviation	29.80	37.10	172.31	29.48	33.30	105.29
CV (%)	6.81	5.35	5.05	6.52	4.42	3.15

Recovery

The recovery of Mouse OPG spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	90-102	96
EDTA plasma (n=8)	88-100	94
Cell culture media (n=8)	87-102	94

Linearity

Samples were spiked with high concentrations of Mouse OPG and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: O08712
Synonyms	TNFRSF11B , OCIF, TR1
Research Area	Cancer, Cardiovascular, Metabolism, Immunology, Signal transduction

Assay Procedures

Citations

Redox Biology (2021) IF: 11.799

CNC-bZIP protein NFE2L1 regulates osteoclast differentiation in antioxidant-dependent and independent manners

DOI: 10.1016/j.redox.2021.102180
PMID: 34763297	Sample: plasma

MOLECULAR THERAPY (2020) IF: 8.9860

CD39 Produced from Human GMSCs Regulates the Balance of Osteoclasts and Osteoblasts through the Wnt/β-Catenin Pathway in Osteoporosis

DOI: 10.1016/j.ymthe.2020.04.003
	Sample: serum,plasma

Biomaterials Science (2022) IF: 7.59

Enhanced ectopic bone formation by strontium-substituted calcium phosphate ceramics through regulation of osteoclastogenesis and osteoblastogenesis

DOI: 10.1039/D2BM00348A
PMID: 36043373	Sample: mMSC

JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36

Anti-perimenopausal osteoporosis effects of Erzhi formula via regulation of bone resorption through osteoclast differentiation: A network pharmacology-integrated experimental study

DOI: 10.1016/j.jep.2021.113815
PMID: 33444724	Sample: Plasma

FASEB JOURNAL (2020) IF: 4.966

Amyloid β peptide promotes bone formation by regulating Wnt/β‐catenin signaling and the OPG/RANKL/RANK system

DOI: 10.1096/fj.201901550R
	Sample: MC3T3-E1 cell

World Journal of Stem Cells (2020) IF: 3.231

CR6-interacting factor-1 contributes to osteoclastogenesis by inducing receptor activator of nuclear factor κB ligand after radiation

DOI: 10.4252/wjsc.v12.i3.222
	Sample: BM-MSCs

LIFE SCIENCES (2018) IF: 3.234

Overexpressed miR-145 inhibits osteoclastogenesis in RANKL-induced bone marrow-derived macrophages and ovariectomized mice by regulation of Smad3

DOI: 10.1016/j.lfs.2018.03.042
	Sample: Serum

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