Current Position:Home>>Kits>>ELISA Kits>> MS Mouse VEGF-A(Vascular Endothelial Cell Growth Factor A) ELISA Kit
Cat.No.:E-MSEL-M0005
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Assay type | Sandwich-ELISA |
Format | 48T/96T/96 |
Assay time | 3.5h |
Detection range | 7.81-500 pg/mL |
Sensitivity | 3.21 pg/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 25μL |
Specificity | This kit recognizes Mouse VEGF-A in samples. No significant cross-reactivity or interference between Mouse VEGF-A and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of Mouse VEGF-A concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
|
Concentrated Biotinylated Detection Ab (50×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
|
Concentrated HRP Conjugate (50×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse VEGF-A were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse VEGF-A were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 22.41 | 58.49 | 215.21 | 20.44 | 50.95 | 220.39 |
Standard deviation | 1.32 | 2.81 | 10.16 | 1.03 | 2.39 | 10.01 |
CV (%) | 5.91 | 4.81 | 4.72 | 5.06 | 4.69 | 4.54 |
The recovery of Mouse VEGF-A spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 99-105 | 102 |
EDTA plasma (n=8) | 93-100 | 96 |
Cell culture media (n=8) | 87-98 | 90 |
Samples were spiked with high concentrations of Mouse VEGF-A and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Database Links | SwissProt:
Q00731
|
Synonyms | VEGF |
Research Area | Cancer, Cardiovascular, Metabolism, Developmental biology, Signal transduction |
PMID: 33398131 |
Sample: RCS tissues |
DOI: 10.1111/cpr.13316 |
|
PMID: 35869570 |
Sample: skin samples |
DOI: 10.1002/glia.24109 |
|
Sample: primary astrocytes,pericytes |
Sample: corneal tissues |
PMID: 30856539 |
Sample: Serum |
PMID: 33918641 |
Sample: Cell sample,Serum |
PMID: 36334391 |
Sample: RAW264.7 cells |
DOI: 10.1002/jbmr.3388 |
|
Sample: primary chondrocytes |
PMID: 35815765 |
Sample: corneal tissue |
Sample: brain microvascular endothelial cells (BMECs) |
PMID: 34413777 |
Sample: muscle,serum |
PMID: 36253797 |
Sample: serum |
PMID: 35447198 |
Sample: serum |
PMID: 35248678 |
Sample: gastrocnemius muscles |
Sample: Serum,Tissue homogenate |
Sample: Mesenchymal stem cells (MSCs) |
Sample: serum |