MTT Assay Kit (Cell Proliferation)[341]

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      Catalog number:E-CK-A341

      Size:
      • 500T
      • 1000T
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      Price: $136

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      Introduction

      Elabscience® MTT Assay Kit is a rapid and highly sensitive kit widely used in cell proliferation and cytotoxicity detection.


      Detection Principle

      MTT is 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide which can be reduced by some dehydrogenases in mitochondria to form a crystalline dark purple product formazan. Formazan can be completely dissolved by DMSO and its absorbance is near 570 nm wavelength. The more and faster the cells proliferate, the darker the color is, and the more cytotoxic, the lighter the color is. For the same cells, there is a linear relationship between the depth darkness of color and the number of cells.


      Components

      Cat.

      Products

      500 Tests

      1000 Tests

      Storage

      E-CK-A341A

      MTT(5 ×)

      5 mL

      10 mL

      2~8°C

      E-CK-A341B

      MTT Substrate

      12.5 mL×2

      10 mL×5

      2~8°C

      Manual

       One Copy



      Reagents Not Included

      DMSO, PBS.


      Instructions

      MTT(5 ×)[ E-CK-A341A] is concentrated, please diluted with MTT Substrate to 1 × MTT working solution before use.

      For example: take 100 μL MTT(5 ×)[ E-CK-A341A], add to 400 μL MTT Substrate[E-CK-A341B], and the mixture is 1 × MTT working solution.


      Storage

      Store at 2~8°C for half a year, MTT (5 ×)[ E-CK-A341A] should be stored in dark.


      Cautions

      1.  For maximal assay performance, this reagent should be used within 6 months. Avoid freeze / thaw cycles.

      2.  Suspension cells must be centrifuged and absorb supernatant before adding DMSO.

      3.  The format of formazan is depend on living cells and incubate time, Mix the cell suspension before adding to the wells to avoid cell precipitation leading to different cell number in each well. The OD value may change rapidly with time when the cell number is large.

      4.  MTT is yellow and needs to be stored in dark. Long-term illumination will lead to failure. Do not use when the color turns grey green. MTT may solidify at 4°C or lower temperature. It can be incubated in water bath at 20~25°C for a short time until it is completely melted before use.

      5.  For your safety and health, please wear the lab coat and disposable gloves before the experiments.

      Citations

      1. Ag-containing antibacterial self-healing micro-arc oxidation coatings on Mg–Zn–Sr alloys
        IF: 2.433
        Journal: Surface Engineering(2020)
        DOI: 10.1080/02670844.2020.1800346
        PMID: 99999999
        Products: E-CK-A341 
        Sample type: Cell culture supernatant
        Reactivity: Mouse
      2. CaMK4-dependent phosphorylation of Akt/mTOR underlies Th17 excessive activation in experimental autoimmune prostatitis
        Journal: The FASEB Journal(2020)
        DOI: 10.1096/fj.201902910RRR
        PMID: 32862457
        Products: E-CK-A341 E-CK-A361 E-EL-M0018 E-EL-M0019 E-EL-M0032 E-EL-M0037 E-EL-M0043 E-EL-M0044 E-EL-M0046 E-EL-M0047 E-EL-M0048 E-EL-M0731 E-EL-M1273 E-EL-M2446 
        Sample type: Cell culture supernatant,Tissue homogenate
        Reactivity: Mouse
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