Neo(Neomycin) ELISA Kit

    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    <
    >
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience

      Catalog number:E-FS-E068

      Size:
      • 96T
      Qty:
      - +
      Price: $276

      sample_type: Tissue,Milk

      Sensitivity: 0.5 ppb

      reactivity: Universal

      Lead Time: Order now, ship in 3 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request

      Test principle

      This kit uses Competitive-ELISA as the method. It can detect Neomycin (Neo) in samples, such as tissue, raw milk, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate in this kit has been pre-coated with coupled antigen. During the reaction, Neo in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-Neo antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and TMB substrate is added for color development. There is a negative correlation between the OD value of samples and the concentration of Neo. The concentration of Neo in the samples can be calculated by comparing the OD of the samples to the standard curve.


      Technical indicator

      Sensitivity: 0.5 ppb (ng/mL)

      Reaction mode: 25, 30 min~ 15 min

      Detection limit: Tissue ---50 ppb, Raw Milk ---20 ppb

      Cross-reactivity: Neomycin--100%, Streptomycin, Kanamycin, Gentamicin---<0.1%, 

      Sample recovery rate: 90%±30%.


      Kits components

      Item

      Specifications

      ELISA Microtiter plate

      96 wells

      Standard Liquid

      1.5mL each

      (0ppb, 0.5ppb, 1.5ppb, 4.5ppb, 13.5ppb, 40.5ppb)

      HRP Conjugate

      7ml

      Antibody Working Solution

      7mL

      Substrate Reagent A

      7mL

      Substrate Reagent B

      7mL

      Stop Solution

      7mL

      20×Concentrated Wash Dilution

      25mL

      5× Concentrated Sample Dilution

      30mL

      Plate Sealer

      3 pieces

      Sealed Bag

      1 piece

      Manual

      1 copy


      Other supplies required

      Instruments:  Microplate Reader, Homogenizer, Oscillators, Centrifuge, Balance (sensibility 0.01 g).

      Micropipettor: Single-channel (20-200 μL, 100-1000 μL).

      Reagents: Trichloroacetic Acid (C2HCl3O2), Concentrated Sulfuric Acid (H2SO4), Sodium Chloride (NaCl)


      Storage and valid period

      Store at 2~8for 1 year. Avoid freeze.

      Please store the opened kit at 2~8, protect from light and moisture. The valid period is 1 months.

      Expiry date:  expiration date is on the packing box.

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      MSDS for ELISA ISO 9001

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