One-step TUNEL In Situ Apoptosis Kit (Green, FITC)
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Price: $ 498
Price: $ 298
Price: $ 198
- Kit Type: Apoptosis/DNA Fragmentation
For research use only. Order now, ship in 3 days
Detection Principle | When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with fluorescence microscope. |
Detection Method | Fluorometric Method |
Sample Type | Paraffin section;frozen section;cell slide |
Assay Time | 3 hours |
Detection Instrument | Fluorescence Microscope |
Dye Type | FITC |
Ex/Em | 490/520 |
Channel Set | FITC |
Filter Set | FITC |
Other Reagents Required | 4% Paraformaldehyde(E-IR-R113),0.2% Triton X-100(E-IR-R122),PBS(E-BC-R187),ddH2O,Mounting solution with anti-fluorescence quencher(E-IR-R119),Xylene,Ethanol |
Storage | This product can be stored at -20°C for 12 months with shading light. |
Expiration date | 12 months |
Shipping | Ice bag |
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Q1:Is it avaliable to take TUNEL and IF assays together?
It is avaliable therometically, but it is recommanded to take a pre-test as there is no such data yet. You might need to notice some key points: 1. Protease K for section sample treatment as permeability agent may interfere the antigen of immunofluorescence; 2. High temperature or high pressure for antigen repair in immunofluorescence assay may lead to DNA fragmentation, that will end up with false positive TUNEL results.
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Q2:Can the One-step TUNEL kit detect a sperm sample?
Yes, it can be used to test sperm samples.
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Q3:How many samples can a 50 Assays kit afford?
A 50 assays kit can detect 50 samples with the size of about 5cm2 (the size of a cover slide or 12-well plate) without positive or negative control. It will take another two assays for control groups.
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Q4:What is the difference between TUNEL in-situ kit and TUNEL flow cytometry kit?
The main difference lies in the detection methods, for TUNEL in-situ kits, a fluorescence microscope is used for observing the results, as for TUNEL flow ytometry kit, the sample will be detected by a flow cytometer. On the other hand, TUNEL in-situ kit is more suitable for tissue sections and cell slides or smears, while TUNEL flow ytometry kit is more suitable for suspended cells and adherent cells.
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Q5:Is there any TUNEL assay kit that can detect apoptosis in erythrocyte?
The detection of TUNEL is based on DNA fragmentation in late stage apoptosis, so it is not avaliable for erythrocyte as there is no nuclei or other organelles containing DNA in erythrocyte.
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Q6:Can DNase I Bufferr be diluted with DEPC water?
It is recommended to dilute with ultra-pure water, DO NOT use DEPC water. DEPC water is used to protect RNA or DNA from enzymatic hydrolysis, it will deactive Dnase I. DEPC water can be used to dissolve RNA when extracting RNA.
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Q7:What is the well state of dewaxing?
The tissue is transparent, and no water drop can be left on the slide.
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Q8:Can the cell sample be tested with the TUNEL kit without being prepared as smears and slides?
Yes, the cells can be collected into EP tubes as cell suspension. After being stained by following the procedure, the cells can be detected by flow cyrometer, or the suspension can be sucked onto a slide and observed with a microscope.
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Q9:Can TUNEL be used to observe apoptosis in Drosophila melanogaster tissues?
There is no species limination in TUNEL assay kits, so it will be avaliable theromatically. It is recommanded for customers to explore the method of sample preparation as there is no such data for insect samples.
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Q10:For TUNEL assays, can a quenched slice be done again?
No. TUNEL works by attaching a fluorescently labeled dUTP to the 3'-OH terminal of the fragmented DNA, it can only be worked once since there is no 3'-OH terminal after the operation.