One-step TUNEL In Situ Apoptosis Kit (Green, FITC) (E-CK-A320)

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Size	Price	Qty
100Assays	$ 498.00	- +
50Assays	$ 298.00	- +
20Assays	$ 198.00	- +

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Detection Principle	When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with fluorescence microscope.
Detection Method	Fluorometric method
Sample Type	Paraffin section;frozen section;cell slide
Assay Time	3 hours
Detection Instrument	Fluorescence Microscope
Dye Type	FITC
Ex/Em	490/520
Channel Set	FITC
Filter Set	FITC
Other Reagents Required	4% Paraformaldehyde(E-IR-R113),0.2% Triton X-100(E-IR-R122),PBS(E-BC-R187),ddH2O,Mounting solution with anti-fluorescence quencher(E-IR-R119),Xylene,Ethanol
Storage	This product can be stored at -20°C for 12 months with shading light.
Expiration date	12 months
Shipping	Ice bag

Cat.No.	Product Name	Sizes
E-CK-A211	Annexin V-FITC/PI Apoptosis Kit	200Assays , 100Assays , 50Assays , 20Assays
E-CK-A301	Mitochondrial Membrane Potential Assay Kit (with JC-1)	100Assays , 50Assays , 20Assays
E-CK-A321	One-step TUNEL In Situ Apoptosis Kit (Green, Elab Fluor^® 488)	100Assays , 50Assays , 20Assays
E-CK-A362	Enhanced Cell Counting Kit 8 (WST-8/CCK8)	500Tests , 100Tests