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One-step TUNEL In Situ Apoptosis Kit (Green, FITC)

 Click to find the fluorescence fit you.

Citations(30)

  • Cat.No.:E-CK-A320

Applications:Apoptosis/DNA Fragmentation

Manual MSDS

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Detection principle

When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with fluorescence microscope.

General Information

Application DNA Fragmentation
Detection method Fluorometric Method
Sample type Paraffin section, frozen section, cell slide
Assay time 3 hours
Detection instrument Fluorescence Microscope
Dye Type FITC
Ex/Em (nm) 490/520
Channel set FITC
Filter set FITC
Other reagents required 4% Paraformaldehyde(E-IR-R113),0.2% Triton X-100(E-IR-R122),PBS(E-IR-R187),ddH2O,Mounting solution with anti-fluorescence quencher(E-IR-R119),Xylene,Ethanol
Storage -20°C, shading light
Shipping Ice bag
Expiration date 12 months

Operation Guide

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. JOURNAL OF NANOBIOTECHNOLOGY (2022) IF: 9.429
    Self-assembly of DNA nanostructure containing cell-specific aptamer as a precise drug delivery system for cancer therapy in non-small cell lung cancer

    DOI: 10.1186/s12951-022-01701-5
  2. Gut Microbes (2022) IF: 9.4339
    Gut microbiota modulate radiotherapy-associated antitumor immune responses against hepatocellular carcinoma Via STING signaling

    DOI: 10.1080/19490976.2022.2119055

    PMID: 36093568
  3. JCI Insight (2023) IF: 9.484
    IL13RA2 downregulation in fibroblast promotes keloid fibrosis via JAK/STAT6 activation

    DOI: 10.1172/jci.insight.157091
  4. Stem Cell Research & Therapy (2022) IF: 8.0790
    UC-MSCs promote frozen-thawed ovaries angiogenesis via activation of the Wnt/β-catenin pathway in vitro ovarian culture system

    DOI: 10.1186/s13287-022-02989-8

    PMID: 35841074
  5. FREE RADICAL BIOLOGY AND MEDICINE (2022) IF: 8.1010
    Sonodynamic therapy reduces cardiomyocyte apoptosis through autophagy activated by reactive oxygen species in myocardial infarction

    DOI: 10.1016/j.freeradbiomed.2022.12.080
  6. Journal of Translational Medicine (2023) IF: 8.44
    Angiotensin-(1–7) ameliorates sepsis-induced cardiomyopathy by alleviating inflammatory response and mitochondrial damage through the NF-κB and MAPK pathways

    DOI: 10.1186/s12967-022-03842-5
  7. Cells (2022) IF: 7.666
    Hypoxia-Induced HIF-1α Expression Promotes Neurogenic Bladder Fibrosis via EMT and Pyroptosis

    DOI: 10.3390/cells11233836
  8. AMERICAN JOURNAL OF CHINESE MEDICINE (2022) IF: 6.005
    Anti-Inflammatory and Anti-oxidative Effects of Puerarin in Postmenopausal Cardioprotection: Roles of Akt and Heme Oxygenase-1

    DOI: 10.1142/S0192415X2350009X
  9. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
    Brca1 Is Regulated by the Transcription Factor Gata3, and Its Silencing Promotes Neural Differentiation in Retinal Neurons

    DOI: 10.3390/ijms232213860
  10. Journal of Inflammation-London (2022) IF: 6.283
    5-Methoxyflavone alleviates LPS-mediated lung injury by promoting Nrf2-mediated the suppression of NOX4/TLR4 axis in bronchial epithelial cells and M1 polarization in macrophages

    DOI: 10.1186/s12950-022-00319-6
  11. BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 6.53
    Antioxidation and Nrf2-mediated heme oxygenase-1 activation contribute to renal protective effects of hydralazine in diabetic nephropathy

    DOI: 10.1016/j.biopha.2022.113139
  12. Frontiers in Cell and Developmental Biology (2021) IF: 6.684
    Fexofenadine Protects Against Intervertebral Disc Degeneration Through TNF Signaling

    DOI: 10.3389/fcell.2021.687024
  13. POSTHARVEST BIOLOGY AND TECHNOLOGY (2022) IF: 6.751
    Methyl salicylate retards mitochondria-mediated programmed cell death in peel spotting of ‘Sucrier’ banana during storage

    DOI: 10.1016/j.postharvbio.2022.112099
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    Moxibustion mitigates mitochondrial dysfunction and NLRP3 inflammatory activation in cyclophosphamide-induced premature ovarian insufficiency rats

    DOI: 10.1016/j.lfs.2022.121283
  15. DEVELOPMENT (2023) IF: 6.862
    An intersectional genetic approach for simultaneous cell type-specific labelling and gene knockout in the mouse

    DOI: 10.1242/dev.201198
  16. INFLAMMATION RESEARCH (2023) IF: 6.986
    circFLNA promotes intestinal injury during abdominal sepsis through Fas-mediated apoptosis pathway by sponging miR-766-3p

    DOI: 10.1007/s00011-023-01688-1
  17. CELL CYCLE (2022) IF: 5.173
    Low-dose aspirin inhibits trophoblast cell apoptosis by activating the CREB/Bcl-2 pathway in pre-eclampsia

    DOI: 10.1080/15384101.2022.2092814

    PMID: 35792905
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    DOI: 10.1016/j.fct.2022.113576
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    Sustained developmental endothelial locus-1 overexpression promotes spinal cord injury recovery in mice through the SIRT1/SERCA2 signaling pathway

    DOI: 10.1016/j.brainresbull.2022.01.016

    PMID: 35093467
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    hUCMSCs reduce theca interstitial cells apoptosis and restore ovarian function in premature ovarian insufficiency rats through regulating NR4A1-mediated mitochondrial mechanisms

    DOI: 10.1186/s12958-022-00992-5

    PMID: 35986315
  23. Fishes (2022) IF: 3.17
    Identification and Characterization of Heme Oxygenase-1 from Litopenaeus vannamei Involved in Antioxidant and Anti-Apoptosis under Ammonia Stress

    DOI: 10.3390/fishes7060356
  24. HUMAN & EXPERIMENTAL TOXICOLOGY (2023) IF: 3.247
    Sevoflurane Postconditioning Attenuates Cerebral Ischemia-Reperfusion Injury by Inhibiting SP1/ACSL4-Mediated Ferroptosis

    DOI: 10.1177/09603271231160477
  25. JOURNAL OF FOOD SCIENCE (2022) IF: 3.693
    Effect of Flammulina velutipes polysaccharides on endoplasmic reticulum stress-mediated apoptosis by activating PLC–IP3 pathway in HepG2 cells

    DOI: 10.1111/1750-3841.16423
  26. EUROPEAN JOURNAL OF NEUROSCIENCE (2022) IF: 3.698
    Repeated sevoflurane exposures inhibit neurogenesis by inducing the upregulation of glutamate transporter 1 in astrocytes

    DOI: 10.1111/ejn.15874
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    Effects of AavLEA1 Protein on Mouse Ovarian Tissue Cryopreservation by Vitrification

    DOI: 10.1089/bio.2021.0048

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    DOI: 10.3892/etm.2022.11680
  30. International Journal of Genomics (2022) IF: 2.758
    The Novel Action of miR-193b-3p/CDK1 Signaling in HCC Proliferation and Migration: A Study Based on Bioinformatic Analysis and Experimental Investigation

    DOI: 10.1155/2022/8755263
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Reviews/Q&A

  • Show all
  • Reviews
  • Q&A
Q

L*********gSubmitted [ Aug 10 2023 ]

Asked: Hello, my name is Tommy from Southern University of Science and Technology (China). This would be the first time I will be using kits and reagents from Elabscience and thus, I would appreciate it if you could send me a free sample of the kit. I am interested in One-step TUNEL Apoptosis Kit (Green, FITC), 50 assays (Cat.No.:E-CK-A320). Would you be able to provide me with a free sample of the kit before I order the 50 assays? Thank you very much. Tommy.

Reply

A

adminSubmitted [ Aug 10 2023 ]

Answered: Please contact our China sales department: zhiliang.x@elabscience.com.cn

Q

M*************DSubmitted [ Dec 28 2022 ]

Asked: Hi, I am using the one step TUNEL Apoptosis Kit (Green, FITC) for my samples. I want to know about the positive and the negative controls used in this assay kit. Is the POSITIVE CONTROL the one I used in my experiment as treated positive control group? Same is with the NEGATIVE CONTROL is it the one I used in my study as negative control group? I request you to please clarify it as early as possible. Thank you

Reply

A

adminSubmitted [ Dec 28 2022 ]

Answered: Both the positive control and the negative control are the control of TUNEL test, which has nothing to do with the grouping in your experiment.

Q

Z*** Submitted [ Jul 20 2022 ]

Asked: Hi, I am trying to find the best assay for apoptosis detection that works with my experimental design. Is it possible to get a free sample to test if it works for me.

Reply

A

adminSubmitted [ Jul 20 2022 ]

Answered: Dear Zuha, thanks for your message. Cat Number: E-CK-A320 One-step TUNEL In Situ Apoptosis Kit (Green, FITC) we can provide you with a trial pack-10 assays for free.

Q

R***nSubmitted [ Jun 13 2022 ]

Asked: Respected Sir, I am Mohd Rihan, working under the department of Pharmacology and Toxicology, NIPER, Mohali, Chandigarh., india. Sir, i am intreseted to purchased your kit, but before ordering your kit, if it possible to check your kit can work or not with my sample. So, if can possible to get a free sample before ordering 50 assay kit and if it work fine with us we will put more order in future. looking your postive response. Thankyou

Reply

A

adminSubmitted [ Jun 13 2022 ]

Answered: Dear Mohd Rihan,thanks for your message,Cat Number: E-CK-A320 One-step TUNEL In Situ Apoptosis Kit (Green, FITC) we can provide you with a trial pack-10 assays for free.

Q

R***nSubmitted [ Jun 13 2022 ]

Asked: Respected Sir, I am Mohd Rihan, working under the department of Pharmacology and Toxicology, NIPER, Mohali, Chandigarh., india. Sir, i am intreseted to purchased your kit, but before ordering your kit, if it possible to check your kit can work or not with my sample. So, if can possible to get a free sample before ordering 50 assay kit and if it work fine with us we will put more order in future. looking your postive response. Thankyou

Reply

A

adminSubmitted [ Jun 13 2022 ]

Answered: Dear Mohd Rihan,thanks for your message,Cat Number: E-CK-A320 One-step TUNEL In Situ Apoptosis Kit (Green, FITC) we can provide you with a trial pack-10 assays for free.

A

adminSubmitted [ Jun 13 2022 ]

Answered: 

Q

H*******.Submitted [ May 04 2022 ]

Asked: Hello--I am in the same situation as the previous posted question--this would be the first product I have purchased from Elabscience and would also be interested in a sample of the kit. Would this be possible? Please let me know. Thank you, Hannah.

Reply

A

adminSubmitted [ May 04 2022 ]

Answered: Hello Hannah, thanks for your message. For your request, a free sample of the kit can be applied for you. Could you please send your detailed shipping address and valid FedEx account (if available) to orders@elabscience.com? Your request will be processed soon after receiving your information. Thank you.

Q

S*************ASubmitted [ Jan 21 2022 ]

Asked: Hello, This would be first time I would be using kits/reagents from Elabscience and therefore, I was wondering if I can get a free sample of a kit I would like to try out. I am interested in One-step TUNEL Apoptosis Kit (Green, FITC), 20 assay (Cat.No.:E-CK-A320). Please let me know if I can get a free sample of it before ordering the kit of 50 assays. Thank you, Shireen

Reply

A

adminSubmitted [ Jan 21 2022 ]

Answered: Hello Shireen, Thanks for your message and interest in our product. For your request, a free sample below has been applied for you: Cat Number: E-CK-A320 One-step TUNEL Apoptosis Kit (Green, FITC) 20 Assays Could you please send us your detailed shipping address and valid FedEx account to cover the shipping fee for the free sample? Your request will be processed soon after receiving your information. Any questions please feel free to contact us at orders@elabscience.com. Best regards.

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