When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with fluorescence microscope.
|Detection method||Fluorometric Method|
|Sample type||Paraffin section, frozen section, cell slide|
|Assay time||3 hours|
|Detection instrument||Fluorescence Microscope|
|Other reagents required||4% Paraformaldehyde(E-IR-R113),0.2% Triton X-100(E-IR-R122),PBS(E-IR-R187),ddH2O,Mounting solution with anti-fluorescence quencher(E-IR-R119)|
|Storage||RT/-20°C. Refer to the Manual.|
|Expiration date||12 months|
|E-CK-A321||One-step TUNEL In Situ Apoptosis Kit (Green, AF488)|
|E-CK-A211||Annexin V-FITC/PI Apoptosis Kit|
|E-CK-A301||Mitochondrial Membrane Potential Assay Kit (with JC-1)|
|E-CK-A311||Caspase 3 Activity Colorimetric Assay Kit|
|E-CK-A362||Enhanced Cell Counting Kit 8 (WST-8/CCK8)|
From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to email@example.com, we will get back to you with the reward after we confirm it ASAP!
H*******.Submitted [ May 04 2022 ]
Asked: Hello--I am in the same situation as the previous posted question--this would be the first product I have purchased from Elabscience and would also be interested in a sample of the kit. Would this be possible? Please let me know. Thank you, Hannah.
adminSubmitted [ May 04 2022 ]
Answered: Hello Hannah, thanks for your message. For your request, a free sample of the kit can be applied for you. Could you please send your detailed shipping address and valid FedEx account (if available) to firstname.lastname@example.org? Your request will be processed soon after receiving your information. Thank you.
S*************ASubmitted [ Jan 21 2022 ]
Asked: Hello, This would be first time I would be using kits/reagents from Elabscience and therefore, I was wondering if I can get a free sample of a kit I would like to try out. I am interested in One-step TUNEL Apoptosis Kit (Green, FITC), 20 assay (Cat.No.:E-CK-A320). Please let me know if I can get a free sample of it before ordering the kit of 50 assays. Thank you, Shireen
adminSubmitted [ Jan 21 2022 ]
Answered: Hello Shireen, Thanks for your message and interest in our product. For your request, a free sample below has been applied for you: Cat Number: E-CK-A320 One-step TUNEL Apoptosis Kit (Green, FITC) 20 Assays Could you please send us your detailed shipping address and valid FedEx account to cover the shipping fee for the free sample? Your request will be processed soon after receiving your information. Any questions please feel free to contact us at email@example.com. Best regards.