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One-step TUNEL In Situ Apoptosis Kit (Red, Elab Fluor^® 594)

Cat.No.:E-CK-A322

Applications:Apoptosis/DNA Fragmentation

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Detection principle

When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with fluorescence microscope.

General Information

Application	DNA Fragmentation
Detection method	Fluorometric Method
Sample type	Paraffin section, frozen section, cell slide
Assay time	3 hours
Detection instrument	Fluorescence Microscope
Dye Type	Elab Fluor^® 594
Ex/Em (nm)	590/617
Filter set	TRITC
Other reagents required	4% Paraformaldehyde(E-IR-R113),0.2% Triton X-100(E-IR-R122),PBS(E-IR-R187),ddH2O,Mounting solution with anti-fluorescence quencher(E-IR-R119),Xylene,Ethanol
Storage	-20°C, shading light
Shipping	Ice bag
Expiration date	12 months

Related Products

E-CK-A321	One-step TUNEL In Situ Apoptosis Kit (Green, Elab Fluor^® 488)
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E-CK-A301	Mitochondrial Membrane Potential Assay Kit (with JC-1)
E-CK-A362	Enhanced Cell Counting Kit 8 (WST-8/CCK8)

Operation Guide

Citations

ACS Nano (2023) IF: 18.027
Supramolecular Hydrogel Microspheres of Platelet-Derived Growth Factor Mimetic Peptide Promote Recovery from Spinal Cord Injury

DOI: 10.1021/acsnano.2c12017

PMID: 36787636
Nature Communications (2022) IF: 17.694
Long-term monitoring of intravital biological processes using fluorescent protein-assisted NIR-II imaging

DOI: 10.1038/s41467-022-34274-w

PMID: 36333308
JOURNAL OF NANOBIOTECHNOLOGY (2022) IF: 9.429
Platelet membrane-camouflaged nanoparticles carry microRNA inhibitor against myocardial ischaemia‒reperfusion injury

DOI: 10.1186/s12951-022-01639-8

PMID: 36195952
Stem Cell Research & Therapy (2022) IF: 8.0790
miR-126-3p containing exosomes derived from human umbilical cord mesenchymal stem cells promote angiogenesis and attenuate ovarian granulosa cell apoptosis in a preclinical rat model of premature ovarian failure

DOI: 10.1186/s13287-022-03056-y

PMID: 35883161
DRUG DELIVERY (2022) IF: 6.419
Sevelamer arsenite nanoparticle as a Pi-responsive drug carrier and embolic agent for chemoembolization

DOI: 10.1080/10717544.2022.2072541
JOURNAL OF ETHNOPHARMACOLOGY (2023) IF: 5.195
The therapeutic mechanism of Yuye decoction on type 2 diabetes mellitus based on network pharmacology and experimental verification

DOI: 10.1016/j.jep.2023.116222
Frontiers in Pharmacology (2022) IF: 5.988
Monomethyl lithospermate alleviates ischemic stroke injury in middle cerebral artery occlusion mice in vivo and protects oxygen glucose deprivation/reoxygenation induced SHSY-5Y cells in vitro via activation of PI3K/Akt signaling.

DOI: 10.3389/fphar.2022.1024439

Sample: Tissue Slice
TOXICOLOGY LETTERS (2023) IF: 4.271
The mechanism of rh-endostatin-induced cardiotoxicity and its protection by dihydromyricetin[in vivo/in vitro, C57BL/6 mice, AC16 and hiPSC-CMs]

DOI: 10.1016/j.toxlet.2023.01.012
RENAL FAILURE (2022) IF: 3.222
Rosiglitazone attenuates hypoxia-induced renal cell apoptosis by inhibiting NF-κB signaling pathway in a PPARγ-dependent manner

DOI: 10.1080/0886022X.2022.2148539
Frontiers in Pediatrics (2022) IF: 3.418
Hyperoxia Induced Bronchopulmonary Dysplasia-Like Inflammation via miR34a-TNIP2-IL-1β Pathway

DOI: 10.3389/fped.2022.805860

Sample: sputum
Biopreservation and Biobanking (2021) IF: 2.3
Effects of AavLEA1 Protein on Mouse Ovarian Tissue Cryopreservation by Vitrification

DOI: 10.1089/bio.2021.0048

PMID: 34788107
Journal of Stroke & Cerebrovascular Diseases (2023) IF: 2.677
Inhibition of PI3K/Akt/mTOR signaling by NDRG2 contributes to neuronal apoptosis and autophagy in ischemic stroke

DOI: 10.1016/j.jstrokecerebrovasdis.2023.106984

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