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Oxaloacetate (OAA) Colorimetric Assay Kit

Cat.No.:E-BC-K901-M
Detection instrument: Microplate reader (402-422 nm, optimum wavelength: 412 nm)

Detection method: Colorimetric method

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Detection principle

Oxaloacetic acid (OAA), one of the intermediates in the tricarboxylic acid cycle is an important substance in carbon and nitrogen metabolism. Oxaloacetate reacts with substrate acetyl-coA under the action of enzyme. The substance produced can react with DTNB, which has the maximum absorption peak at 412 nm. The content of oxaloacetate can be determined by measuring the absorbance value.

Performance characteristics

Sample type	animal tissue
Sensitivity	0.017 mmol/L
Detection range	0.017-1 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	15 min
Precision	Average inter-assay CV: 2.300%Average intra-assay CV: 2.100%
Other instruments required	Normal saline (0.9% NaCl)
Other reagents required	Incubator, Ultrafiltration tube (50kD)
Storage	-20℃
Valid period	12 months

Ralated product

Cat Number	Product Name
E-BC-K650-M	Pyruvate Dehydrogenase (PDH) Activity Assay Kit
E-BC-K178-M	Citrate Synthase(CS) Activity Assay Kit
E-BC-K652-M	Acetyl-CoA Colorimetric Assay Kit
E-BC-K083-M	α-Ketoglutarate Dehydrogenase(α-KGDH) Activity Assay Kit
E-BC-K651-M	NAD-Isocitrate Dehydrogenase (NAD-IDH) Activity Assay Kit

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

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