Oxaloacetate Colorimetric Assay Kit (E-BC-K901-M)
For research use only.
| Detection Principle | Oxaloacetic acid (OAA), one of the intermediates in the tricarboxylic acid cycle is an important substance in carbon and nitrogen metabolism. Oxaloacetate reacts with substrate acetyl-coA under the action of enzyme. The substance produced can react with DTNB, which has the maximum absorption peak at 412 nm. The content of oxaloacetate can be determined by measuring the absorbance value. |
| Sample Type | Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (402-422 nm, optimum wavelength: 412 nm) |
| Research Area | Tricarboxylic Acid（TCA）Cycle , Energy Metabolism |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.017 mmol/L |
| Detection Range | 0.017-1 mmol/L |
| Precision | inter-assay CV: 2.3% | intra-assay CV: 2.1% |
| Sample Volume | 20 μL(tissue homogenate) |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Rat liver tissue homogenate | 1 |
| 10% Rat brain tissue homogenate | 1 |
| 10% Rat lung tissue homogenate | 1 |
| 10% Mouse liver tissue homogenate | 1 |
| 10% Mouse kidney tissue homogenate | 1 |
| 10% Mouse leg muscle tissue homogenate | 1 |
| 10% Pig heart tissue homogenate | 1 |
| 10% Human urine tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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