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Oxaloacetate (OAA) Colorimetric Assay Kit

  • Cat.No.:E-BC-K901-M

  • Detection instrument: Microplate reader (402-422 nm, optimum wavelength: 412 nm)

To Purchase E-BC-K901-M

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Price: $520

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Detection principle

Oxaloacetic acid (OAA), one of the intermediates in the tricarboxylic acid cycle is an important substance in carbon and nitrogen metabolism. Oxaloacetate reacts with substrate acetyl-coA under the action of enzyme. The substance produced can react with DTNB, which has the maximum absorption peak at 412 nm. The content of oxaloacetate can be determined by measuring the absorbance value.

Performance characteristics

Sample type animal tissue
Sensitivity 0.017 mmol/L
Detection range 0.017-1 mmol/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 15 min
Precision Average inter-assay CV: 2.300%Average intra-assay CV: 2.100%
Other instruments required Normal saline (0.9% NaCl)
Other reagents required Incubator, Ultrafiltration tube (50kD)
Storage -20℃
Valid period 12 months


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