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PE Anti-Mouse Foxp3 Antibody[3G3]

  • Cat.No.:E-AB-F1238D

  • Applications: ICFCM
  • Isotype: Mouse IgG1, κ
  • Host: Mouse

To Purchase E-AB-F1238D

Size:
  • 50Tests
  • 100Tests
  • 100Tests×2
Price: $144
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Antigen Details

Alternate Names Forkhead box protein P3,IPEX,JM2,Foxp3
Swissprot Q99JB6
Gene ID 50943,20371,317382

Product Details

Form Liquid
Clone No. 3G3(See Other Available Formats)
Host Mouse
Isotype Mouse IgG1, κ
Isotype Control PE Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792D]
Reactivity Mouse
Application ICFCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation PE
PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

FOXP3 is a 50-55 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4+/CD25-cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in 'scurfy' mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse, two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical full-length sequence; the other (FOXP3 δ2) lacking exon 2.

Protocols & Troubleshootings

Related Protocols Staining Intracellular Antigens for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Product Name Sizes Price
FITC Anti-Mouse Foxp3 Antibody[3G3] 50Tests, 100Tests, 100Tests×2 Check More
APC Anti-Mouse Foxp3 Antibody[3G3] 50Tests, 100Tests, 100Tests×2 Check More

Citations

  1. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
    Regulating Th17/Treg Balance Contributes to the Therapeutic Effect of Ziyuglycoside I on Collagen-Induced Arthritis

    DOI: 10.3390/ijms232416105

    Sample: spleen
  2. Cancers (2022) IF: 6.575
    IL-2K35C-moFA, a Long-Acting Engineered Cytokine with Decreased Interleukin 2 Receptor α Binding, Improved the Cellular Selectivity Profile and Antitumor Efficacy in a Mouse Tumor Model

    DOI: 10.3390/cancers14194742

    Sample: Spleen single-cell
  3. Frontiers in Molecular Biosciences (2020) IF: 4.188
    Injecting Immunosuppressive M2 Macrophages Alleviates the Symptoms of Periodontitis in Mice

    DOI: 10.3389/fmolb.2020.603817

    PMID: 33195441

    Sample: Cell sample
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