Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> PE/Cyanine7 Anti-Mouse CD40 Antibody[FGK4.5/FGK45]
Cat.No.:E-AB-F1028UH
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Alternate Names | Tumor necrosis factor receptor superfamily member 5,Cd40,B-cell surface antigen CD40,Bp50,CD40L receptor,CD40,Tnfrsf5 |
Swissprot | P27512 |
Gene ID | 21939 |
References |
1. Hasbold J, Johnson-Leger C, Atkins CJ, Clark EA, Klaus GG. Eur J Immunol. 1994; 24(8):1835-1842.
2. Hasbold J, Klaus GG. Eur J Immunol. 1994; 24(1):152-157. 3. Klaus GG, Holman M, Hasbold J. Eur J Immunol. 1994; 24(11):2714-2719. 4. Noelle RJ, Ledbetter JA, Aruffo A. Immunol Today. 1992; 13(11):431-433. 5. Parry SL, Hasbold J, Holman M, Klaus GG. J Immunol. 1994; 152(6):2821-2829. |
Form | Liquid |
Clone No. | FGK4.5/FGK45(See Other Available Formats) |
Host | Rat |
Isotype | Rat IgG2a, κ |
Isotype Control | PE/Cyanine7 Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09833H] |
Reactivity | Mouse |
Application | FCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume]. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | PE/Cyanine7 |
PE/Cyanine7 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 775 nm (e.g., a 780/60 nm bandpass filter). | |
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How to select the appropriate detection channel through the spectrogram?
CD40 is a 48 kD type I transmembrane glycoprotein also known as Bp50. It is a member of the tumor necrosis factor receptor (TNFR) superfamily and is expressed on B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34+ hematopoietic progenitors. CD40 regulates B cell development/maturation, Ig isotype switching and, in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39), which is expressed on activated T cells, is important in costimulation and immune regulation. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
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