PGI2(Prostacyclin) ELISA Kit (E-EL-0022)

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal PGI2. During the reaction, Universal PGI2 in the sample or standard competes with a fixed amount of Universal PGI2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal PGI2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal PGI2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Prostacyclin (PGI2) is involved in platelet aggregation, vasoconstriction, and reproductive functions. PGI2 has a half life of 60 minutes in plasma but only 2 to 3 minutes in buffer. The production of PGI2 is typically monitored by measurement of 6k-PGF1α and 2,3d-6k- PGF1α. 6-keto- PGF1α is produced by the non-enzymatic hydration of PGI2, and is further metabolized in urine to the 2,3-dinor derivative. A number of pharmaceuticals alter and无or inhibit the synthesis of PGI2. Methods to measure PGI2 in urine typically involve HPLC, gas chromatography无mass spectrometry, or radioimmunoassay and enzyme immunoassay for 6k- PGF1α.