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PGI2(Prostacyclin) ELISA Kit

Cat.No.:E-EL-0022
Reactivity: Universal

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96T
48T
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96T*5
96T*10

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Product Details

Properties

Assay type	Competitive-ELISA
Format	96T/48T
Assay time	2.0h
Detection range	31.25-2000 pg/mL
Sensitivity	18.75 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 50μL
Specificity	This kit recognizes PGI2 in samples. No significant cross-reactivity or interference between PGI2 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of PGI2 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with PGI2. During the reaction, PGI2 in the sample or standard competes with a fixed amount of PGI2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to PGI2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PGI2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8℃
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level PGI2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level PGI2 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	94.64	246.44	963.63	90.56	267.93	1038.28
Standard deviation	5.82	11.68	44.91	5.80	15.86	39.56
CV (%)	6.15	4.74	4.66	6.40	5.92	3.81

Recovery

The recovery of PGI2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	90-102	95
EDTA plasma(n=8)	91-102	96
Cell culture media(n=8)	85-96	91

Linearity

Samples were spiked with high concentrations of PGI2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Research Area

Cardiovascular

Assay Procedures

	1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C
	2. Aspirate and wash the plate for 3 times
	3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	5. Add 50μL Stop Solution
	6. Read the plate at 450nm immediately. Calculation of the results

Citations

Chinese Medicine (2021) IF: 5.455
Anti-platelet aggregation of Panax notoginseng triol saponins by regulating GP1BA for ischemic stroke therapy

DOI: 10.1186/s13020-021-00424-3

PMID: 33468191
Sample: Serum
Frontiers in Pharmacology (2021) IF: 5.811
Ginsenoside Rg1-Notoginsenoside R1-Protocatechuic Aldehyde Reduces Atherosclerosis and Attenuates Low-Shear Stress-Induced Vascular Endothelial Cell Dysfunction

DOI: 10.3389/fphar.2020.588259

PMID: 33568993
Sample: Serum
Scientific Reports (2021) IF: 4.38
Vitamin D stimulates miR-26b-5p to inhibit placental COX-2 expression in preeclampsia

DOI: 10.1038/s41598-021-90605-9

PMID: 34045549
Sample: Tissue homogenate
Contrast Media & Molecular Imaging (2022) IF: 3.009
Regulation of the Proliferation of Diabetic Vascular Endothelial Cells by Degrading Endothelial Cell Functional Genes with QKI-7

DOI: 10.1155/2022/6177809

Sample: endothelial cell
Drug Design Development and Therapy (2020) IF: 3.216
Dexamethasone-Loaded Thermosensitive Hydrogel Suppresses Inflammation and Pain in Collagen-Induced Arthritis Rats

DOI: 10.2147/DDDT.S256850

PMID: 33116399
Sample: Serum
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS (2020) IF: 3.561
Microsomal Prostaglandin E2 Synthase-1 Deletion Attenuates Isoproterenol-Induced Myocardial Fibrosis in Mice

DOI: 10.1124/jpet.120.000023

Sample: Plasma
JOURNAL OF BIOMATERIALS APPLICATIONS (2018) IF: 2.082
Fibronectin modified TiO2 nanotubes modulate endothelial cell behavior:

DOI: 10.1177/0885328218774512

Sample: Cells
Food & Nutrition Research (2015) IF: 2.162
N-Acetylneuraminic acid attenuates hypercoagulation on high fat diet-induced hyperlipidemic rats

DOI: 10.3402/fnr.v59.29046

Sample: Serum
ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY (2020) IF: 2.575
Angiotensin converting enzyme inhibitor potentiates the hypoglycaemic effect of NG-nitro-L-arginine methyl ester (L-NAME) in rats

DOI: 10.1080/13813455.2020.1780263

PMID: 32584611
Sample: Serum

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Reviews/Q&A

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Reviews
Q&A

s****aSubmitted [ Sep 07 2021 ]

Asked: Hello I would like to know the reference number of PIG2 Micro Elisa plate (dismountable) for an individual purchase (not together the total ELISA kit). Thanks!

adminSubmitted [ Sep 07 2021 ]

Answered: Sorry, the microplate of PGI2(Prostacyclin) ELISA Kit is not for an individual purchase.

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