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Cat.No.:E-AB-F1195A
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Alternate Names | PAS2,MNS,HGpMiX,GYPA,GPSAT |
Swissprot | P10747 |
References |
1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. June CH, et al. 1994. Immunol. Today 15:321. 3. Linskey PS, et al. 1993. Annu. Rev. Immunol. 11:191. |
Form | Liquid |
Clone No. | CD28.2(See Other Available Formats) |
Host | Mouse |
Isotype | Mouse IgG1, κ |
Isotype Control | Purified Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09793A] |
Reactivity | Human |
Application | FCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | Unconjugated |
How to select the appropriate detection channel through the spectrogram?
CD28 is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the immunoglobulin superfamily and is also known as T44 or Tp44. CD28 is expressed on most T lineage cells, NK cell subsets, and plasma cells. CD28 binds both CD80 and CD86 using a highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death. In vitro studies indicate that ligation of CD28 on T cells by CD80 and CD86 on antigen presenting cells provides a costimulatory signal required for T cell activation and proliferation. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
Product Name | Sizes | Price |
AF/LE Purified Anti-Human CD28 Antibody[CD28.2] | 50μg, 500μg, 1mg | Check More |
FITC Anti-Human CD28 Antibody[CD28.2] | 20Tests, 100Tests, 100Tests×2 | Check More |
PE Anti-Human CD28 Antibody[CD28.2] | 20Tests, 100Tests, 100Tests×2 | Check More |
APC Anti-Human CD28 Antibody[CD28.2] | 20Tests, 100Tests, 100Tests×2 | Check More |
PE/Cyanine5 Anti-Human CD28 Antibody[CD28.2] | 20Tests, 100Tests, 100Tests×2 | Check More |
PE/Cyanine7 Anti-Human CD28 Antibody[CD28.2] | 20Tests, 100Tests, 100Tests×2 | Check More |
PerCP/Cyanine5.5 Anti-Human CD28 Antibody[CD28.2] | 20Tests, 100Tests, 100Tests×2 | Check More |
Elab Fluor® 488 Anti-Human CD28 Antibody[CD28.2] | 20Tests, 100Tests, 100Tests×2 | Check More |
Elab Fluor® 647 Anti-Human CD28 Antibody[CD28.2] | 20Tests, 100Tests, 100Tests×2 | Check More |
PE/Elab Fluor® 594 Anti-Human CD28 Antibody[CD28.2] | 20Tests, 100Tests, 100Tests×2 | Check More |