Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> Purified Anti-Mouse CD4 Antibody[GK1.5] E-AB-F1097A
Cat.No.:E-AB-F1097A
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Alternate Names | T-cell surface glycoprotein CD4,CD4,T-cell surface antigen T4/Leu-3,CD4 |
Swissprot | P06332 |
References |
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579. 3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645. |
Form | Liquid |
Clone No. | GK1.5(See Other Available Formats) |
Host | Rat |
Isotype | Rat IgG2b, κ |
Isotype Control | Purified Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F09843A] |
Reactivity | Mouse |
Application | FCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | Unconjugated |
How to select the appropriate detection channel through the spectrogram?
CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosin kinase, lck. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
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