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Pyruvic Acid Colorimetric Assay Kit

Cat.No.:E-BC-K130-S
Detection instrument: Spectrophotometer(505 nm)

Detection method: Colorimetric method

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Detection principle

Pyruvic acid can react with chromogenic agent and the reaction product is reddish brown in alkaline solution. The depth of color is directly proportional to the pyruvate content. The pyruvate content can be calculated by measuring the OD value at 505 nm.

Performance characteristics

Sample type	Serum,plasma,tissue
Sensitivity	0.006 μmol/mL
Detection range	0.006-2.0 μmol/mL
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	60 min
Precision	Average inter-assay CV: 1.500%Average intra-assay CV: 1.300%
Other instruments required	Micropipettor, Vortex mixer, Incubator
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

Images

Both EATO and NHTO significantly increased the pyruvate concentration. (***P<0.001, *P<0.01 vs. Control)

T M Salman et al investigate the pathways and mechanism of hypoglycemic effect with Telfairia occidentails. Pyruvic acid levels in rat plasma was determined using pyruvic acid colorimetric assay kit (E-BC-K130-S).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.006-2.0 μmol/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Mouse serum	1
Mouse plasma	1
10% Mouse liver tissue homogenization	1
10% Rat kidney tissue homogenization	1
10% Rat heart tissue homogenization	1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Nano Today (2022) IF: 18.962
An acid degradable, lactate oxidizing nanoparticle formulation for non-small cell lung cancer virotherapy

DOI: 10.1016/j.nantod.2022.101582

Sample: peripheral tumor
Frontiers in Molecular Biosciences (2022) IF: 6.113
Glucose Deprivation Induced by Acarbose and Oncolytic Newcastle Disease Virus Promote Metabolic Oxidative Stress and Cell Death in a Breast Cancer Model

DOI: 10.3389/fmolb.2022.816510

PMID: 35936786
Sample: mammary adenocarcinoma tissue
BIOCHIMIE (2022) IF: 4.079
Glucose deprivation using 2-deoxyglucose and acarbose induce metabolic oxidative stress and apoptosis in female mice bearing breast cancer

DOI: 10.1016/j.biochi.2022.01.007

PMID: 35066100
Sample: breast tumor tissue

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