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Cat.No.:E-BC-K130-S
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Protocols for metabolism assays
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Sample type | Serum,plasma,tissue |
Sensitivity | 0.006 μmol/mL |
Detection range | 0.006-2.0 μmol/mL |
Detection method | Colorimetric method |
Assay type | Quantitative |
Assay time | 60 min |
Precision | Average inter-assay CV: 1.500%Average intra-assay CV: 1.300% |
Other instruments required | Micropipettor, Vortex mixer, Incubator |
Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
Storage | 2-8℃ |
Valid period | 12 months |
Both EATO and NHTO significantly increased the pyruvate concentration. (***P<0.001, *P<0.01 vs. Control)
T M Salman et al investigate the pathways and mechanism of hypoglycemic effect with Telfairia occidentails. Pyruvic acid levels in rat plasma was determined using pyruvic acid colorimetric assay kit (E-BC-K130-S).
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.006-2.0 μmol/mL).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type |
Dilution factor |
Human serum |
1 |
Mouse serum |
1 |
Mouse plasma |
1 |
10% Mouse liver tissue homogenization |
1 |
10% Rat kidney tissue homogenization |
1 |
10% Rat heart tissue homogenization |
1 |
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
Sample: peripheral tumor |
PMID: 35936786 |
Sample: mammary adenocarcinoma tissue |
PMID: 35066100 |
Sample: breast tumor tissue |