QuicKey Pro Sheep E2(Estradiol) ELISA Kit

Size	Price	Qty
96T	$ 520.00
48T	$ 416.00
24T	$ 150.00
96T*10	Inquire	/
96T*5	Inquire	/

Product Summary

The sample size of MS series ELISA kit is only 1/4 of that of the traditional sandwich ELISA kit, which can effectively solve the problem of insufficient sample size.

Sensitivity	12.74 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	50 μL
Total Assay Time	1 h 30 min
Reacitivity	Sheep
Specificity	This kit recognizes Sheep E2 in samples.No significant cross-reactivity or interference between Sheep E2 and analogues was observed
Recovery	80%-120%
Sample Type	serum, plasma
Detection Method	Colorimetric method, ELISA, Competitive
Assay Type	Competitive-ELISA
Size	96T / 48T / 24T / 96T10 / 96T5
Storage	2-8℃

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Sheep E2. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Sheep E2 are added to the micro ELISA plate wells. Sheep E2 in samples (or standards) competes with a fixed amount of E2 on the solid phase supporter for sites on the HRP linked detection antibody specific to E2. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Sheep E2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Background

Evidence, accumulated over the past 25 years, shows that estradiol modulates cognitive function in animals and humans. Modulation begins in utero when estrogens direct sexual differentiation of various brain regions controlling reproduction and some cognitive functions. Gonadal hormone influences on the nervous system, including both estrogens and androgens, continue through adolescence and reach their zenith at adulthood when gonadal hormone secretions reach the highest levels. With aging, levels of circulating estrogens plummet and appear to contribute to age-related declines in learning and memory function. These hormone-dependent effects on cognition over the lifespan have been attributed to classic, genomic mechanisms — hormone binding to specific receptors, alterations in gene transcription and the initiation of organ-specific effects in target areas in the brain and peripheral organs. More recently, it has also been established that gonadal hormones, including estradiol, can act at membrane receptors to activate intra-cellular signaling mechanisms which alter cellular function. Moreover, there is increasing documentation that gonadal hormones are neurosteroids, that is, estrogens and androgens are synthesized locally in brain areas and thereby rapidly alter cognition and other neural functions. The physiological functions and implications for this mode of estrogen action are unknown at this time but present a novel area for investigation. Finally, in a more global way, estradiol also acts indirectly to maintain cognitive functions because it exerts neurotrophic effects within the brain. Thus, gonadal hormones, estradiol in particular, have a rich repertoire of possible mechanisms for influencing cognition.

Protein Alias --TODO	12.74 pg/mL
Research Area	Cell Biology ， Metabolism

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QuicKey Pro Sheep E2(Estradiol) ELISA Kit (E-OSEL-S0001)

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