Product Summary

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Sensitivity	2.54 ng/mL
Detection Range	6.25-400 ng/mL
Sample Volume	50 μL
Total Assay Time	1 h 30 min
Reactivity	Bovine
Specificity	This kit recognizes Bovine Cortisol in samples.No significant cross-reactivity or interference between Bovine Cortisol and analogues was observed
Recovery	80%-120%
Sample Type	serum, plasma
Detection Method	Colorimetric method, ELISA, Competitive
Assay Type	Competitive-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	6 months

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Bovine Cortisol. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Bovine Cortisol are added to the micro ELISA plate wells. Bovine Cortisol in samples (or standards) competes with a fixed amount of Cortisol on the solid phase supporter for sites on the HRP linked detection antibody specific to Cortisol. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Bovine Cortisol in the samples is then determined by comparing the OD of the samples to the standard curve.

Background

Cortisol is the primary endogenous adrenal steroid in most mammals, including humans, whereas corticosterone is the primary adrenal corticosteroid in laboratory rodents . Rats and mice do not produce appreciable cortisol, because they lack the adrenocortical zona fasciculata enzyme 17-α hydroxylase (CYP17) . The absence of 17,20 lyase activity of CYP17 also accounts for the lack of adrenal androgen production in laboratory rodents . However, most mammals, including humans, produce both cortisol and corticosterone, albeit the latter at lower circulating concentrations . More importantly, there is functional, evolutionary, and clinical significance to the difference in the structures of cortisol and corticosterone demonstrated by their differing affinities for the glucocorticoid and mineralocorticoid receptors . Furthermore, the inactive steroid cortisone is also found in significant concentrations in cortisol-producing species, including humans .

Research Area

Cell Biology , Metabolism