Product Summary

Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Sensitivity	23.24 pg/mL
Detection Range	78.13-5000 pg/mL
Sample Volume	50 μL
Total Assay Time	1 h 30 min
Reactivity	Bovine
Specificity	This kit recognizes Bovine Pg in samples.No significant cross-reactivity or interference between Bovine Pg and analogues was observed
Recovery	80%-120%
Sample Type	serum, plasma
Detection Method	Colorimetric method, ELISA, Competitive
Assay Type	Competitive-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	6 months

Performance

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Bovine Pg. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Bovine Pg are added to the micro ELISA plate wells. Bovine Pg in samples (or standards) competes with a fixed amount of Pg on the solid phase supporter for sites on the HRP linked detection antibody specific to Pg. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Bovine Pg in the samples is then determined by comparing the OD of the samples to the standard curve.

Background

Progesterone (P4) plays a central role in reproductive events associated with the establishment and maintenance of pregnancy in all mammals. In cattle, P4 is synthesized mainly by the corpus luteum after ovulation, and the corpus luteum remains the major source of circulating P4 throughout pregnancy, with an additional contribution from the placenta in later gestation. P4 is needed during early embryonic development for implantation and placentation; insufficient P4 concentrations can lead to failure of pregnancy and early embryonic loss. P4 concentrations in plasma or milk have been used not only to determine the pregnancy status of the cow but also to monitor ovarian cyclicity and luteal function during the estrous cycle. Thus, the determination of serum or plasma P4 concentrations in cattle is important not only for the early diagnosis of pregnancy, but also for the evaluation of luteal function and the reproductive management of the herd. Many laboratories use radioimmunoassays (RIAs) for the quantification of plasma P4, but RIA, although very sensitive, is expensive and requires the availability of specialized laboratories and personnel.

Research Area

SignalTransduction