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QuicKey Pro Human FE(Ferritin) ELISA Kit

Citations(8)Uniprot : P02792,P02794

Cat.No.:E-OSEL-H0003
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T/24
Assay time	1.5h
Detection range	62.50-4000 pg/mL
Sensitivity	24.31 pg/mL
Sample type &Sample volume	serum and plasma; 50μL
Specificity	This kit recognizes FE in samples. No significant cross-reactivity or interference between FE and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of FE concentrations in serum and plasma.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FE. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human FE are added to the micro ELISA plate wells. Human FE in samples (or standards) combines with the coated antibody and HRP linked detection antibody specific to FE. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Human FE in the samples is then determined by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the table.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips 24T: 8 wells ×3 strips 96T*5: 5 plates, 96T	2-8℃, 1 months
Reference Standard	96T: 2 vials 48T/24T: 1 vial 96T*5: 10 vials	2-8℃, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100×)	96T: 1 vial, 60 μL 48T/24T: 1 vial, 30 μL 96T*5: 5 vials, 60 μL	2-8℃(Protect from light)
Reference Standard & Sample Diluent	96T/48T/24T: 1 vial, 20 mL 96T*5: 5 vials, 20 mL	2-8℃
HRP Linked Ab Diluent	96T/48T/24T: 1 vial, 14 mL 96T*5: 5 vials, 14 mL
Concentrated Wash Buffer(25×)	96T/48T/24T: 1 vial, 30 mL 96T*5: 5 vials, 30 mL
Substrate Reagent	96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL	2-8℃(Protect from light)
Stop Solution	96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL	2-8℃
Plate Sealer	96T/48T/24T: 5 pieces 96T*5: 25 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level FE were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level FE were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	183.00	362.63	1422.41	190.21	332.97	1312.84
Standard deviation	12.04	15.56	58.60	10.44	16.62	69.19
CV (%)	6.58	4.29	4.12	5.49	4.99	5.27

Recovery

The recovery of FE spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	87-101	92
EDTA plasma(n=8)	90-105	97
Urine(n=8)	91-105	99

Linearity

Samples were spiked with high concentrations of Human FE and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P02792,P02794
Synonyms	Ferritin，FE
Research Area	Cancer, Cardiovascular, Metabolism, Neuroscience, Signal transduction

Assay Procedures

Citations

ANNALS OF MEDICINE (2022) IF: 5.348
Iron metabolism abnormalities in autoimmune hemolytic anemia and Jianpishengxue keli can ameliorate hemolysis and improve iron metabolism in AIHA mouse models

DOI: 10.1080/07853890.2022.2157475

Sample: serum
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM (2020) IF: 5.399
Dapagliflozin Suppresses Hepcidin And Increases Erythropoiesis

DOI: 10.1210/clinem/dgaa057

Sample: plasma
Vaccines (2022) IF: 4.422
Immunogenicity of The BNT162b2 COVID-19 mRNA and ChAdOx1 nCoV-19 Vaccines in Patients with Hemoglobinopathies

DOI: 10.3390/vaccines10020151

Sample: serum
PLoS One (2022) IF: 3.24
Effect of vitamin D3-fortified fruit juice supplementation of 4000 IU daily on the recovery of iron status in childbearing-aged women with marginally low iron stores: Protocol for an 8-week, parallel group, double-blind randomized controlled trial

DOI: 10.1371/journal.pone.0265772

PMID: 35333885
Sample: plasma
BRITISH JOURNAL OF NUTRITION (2019) IF: 3.319
Vitamin D3 supplementation for 8 weeks leads to improved haematological status following the consumption of an iron-fortified breakfast cereal: a double-blind randomised controlled trial in iron-deficient women

DOI: 10.1017/S0007114519000412

Sample: Plasma
JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY (2021) IF: 3.849
Increased zinc and albumin but lowered copper in children with transfusion-dependent thalassemia

DOI: 10.1016/j.jtemb.2021.126713

Sample: serum
BMC Endocrine Disorders (2017) IF: 2.275
Insulin treatment corrects hepcidin but not YKL-40 levels in persons with type 2 diabetes mellitus matched by body mass index, waist-to-height ratio, C-reactive protein and Creatinine

DOI: 10.1186/s12902-017-0204-4

Sample: Serum
Journal of Taibah University Medical Sciences (2022)
Prevalence of anemia in pre-school tribal children with reference to parasitic infections and nutritional impact

DOI: 10.1016/j.jtumed.2022.05.002

Sample: blood

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