QuicKey Pro Human IL-6(Interleukin 6) ELISA Kit (E-OSEL-H0001)
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For research use only.
Product Summary
Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.
| Sensitivity | 1.02 pg/mL |
| Detection Range | 3.13-200 pg/mL |
| Sample Volume | 50 μL |
| Total Assay Time | 1 h 30 min |
| Reactivity | Human |
| Specificity | This kit recognizes Human IL-6 in samples.No significant cross-reactivity or interference between Human IL-6 and analogues was observed |
| Recovery | 80%-120% |
| Sample Type | serum, plasma |
| Detection Method | Colorimetric method, ELISA, Sandwich |
| Assay Type | Sandwich-ELISA |
| Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
| Storage | 2-8℃ |
| Expiration Date | 6 months |
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-6. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human IL-6 are added to the micro ELISA plate wells. Human IL-6 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human IL-6.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human IL-6. The concentration of Human IL-6 in the samples is then determined by comparing the OD of the samples to the standard curve.
Background
| Gene ID | 3569 |
| Uniport ID | P05231 |
| Research Area | Cancer , Metabolism , Immunology , Neuroscience |
| Cat.No. | Product Name | Clone No. |
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