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Cat.No.:E-OSEL-H0014
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Assay type | Sandwich-ELISA |
Format | 96T/48T/24 |
Assay time | 1.5h |
Detection range | 15.63-1000 pg/mL |
Sensitivity | 9.38 pg/mL |
Sample type &Sample volume | serum and plasma; 50μL |
Specificity | This kit recognizes IL-8 in samples. No significant cross-reactivity or interference between IL-8 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of IL-8 concentrations in serum and plasma. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 24T: 8 wells ×3 strips 96T*5: 5 plates, 96T |
2-8℃, 1 months |
Reference Standard | 96T: 2 vials
48T/24T: 1 vial 96T*5: 10 vials |
2-8℃, use the reconstituted standard within 24h |
Concentrated HRP Linked Detection Ab(100×) | 96T: 1 vial, 60 μL
48T/24T: 1 vial, 30 μL 96T*5: 5 vials, 60 μL |
2-8℃(Protect from light) |
Reference Standard & Sample Diluent | 96T/48T/24T: 1 vial, 20 mL 96T*5: 5 vials, 20 mL |
2-8℃ |
HRP Linked Ab Diluent | 96T/48T/24T: 1 vial, 14 mL 96T*5: 5 vials, 14 mL |
|
Concentrated Wash Buffer(25×) | 96T/48T/24T: 1 vial, 30 mL 96T*5: 5 vials, 30 mL |
|
Substrate Reagent | 96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL |
2-8℃(Protect from light) |
Stop Solution | 96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL |
2-8℃ |
Plate Sealer | 96T/48T/24T: 5 pieces 96T*5: 25 pieces |
|
Product Description | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level IL-8 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level IL-8 were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 54.94 | 152.77 | 425.52 | 55.10 | 154.08 | 436.69 |
Standard deviation | 3.28 | 6.97 | 16.13 | 3.07 | 8.67 | 14.89 |
CV (%) | 5.97 | 4.56 | 3.79 | 5.58 | 5.63 | 3.41 |
The recovery of IL-8 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 91-103 | 97 |
EDTA plasma(n=8) | 86-97 | 92 |
Samples were spiked with high concentrations of Human IL-8 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Database Links | SwissProt: P10145 |
Synonyms | IL8/CXCL8 |
Research Area | Cancer, Cardiovascular, Immunology |
PMID: 33015716 |
Sample: Cell culture supernatant |
DOI: 10.7150/thno.58321 |
|
PMID: 33995679 |
Sample: Tissue homogenate |
PMID: 35768823 |
Sample: cerebrospinal fluid (CSF),peripheral monocytes |
PMID: 35365616 |
Sample: HCT-116 cell,HT-29 cell |
PMID: 34999169 |
Sample: serum,THP-1 cell,human circulating CD14+ monocytes |
PMID: 35936005 |
Sample: RPE cell |
PMID: 35217799 |
Sample: serum |
PMID: 30448228 |
Sample: Cell culture supernatant |
PMID: 34175826 |
Sample: hASMCs cells |
DOI: 10.1039/D2FO00477A |
|
Sample: serum |
PMID: 36145700 |
Sample: HaCaT cell |
Sample: Saliva |
PMID: 35870375 |
Sample: MH7A cell |
PMID: 33363140 |
Sample: Tissue homogenate |
DOI: 10.1111/cpr.12673 |
|
Sample: CaCO2 cells,HT‐29 cell |
PMID: 34740599 |
Sample: HaCaT cells |
DOI: 10.1042/CS20200047 |
|
PMID: 32219341 |
Sample: Cell culture supernatant |
DOI: 10.1038/srep23103 |
|
Sample: Cell culture supernatant |
Sample: Serum |
DOI: 10.1002/jcp.29713 |
|
PMID: 32385929 |
Sample: Cell culture supernatant |
PMID: 31252119 |
Sample: Cell culture supernatant |
PMID: 35462904 |
Sample: plasma |
Sample: periodontal ligament stem cells (PDLSCs) |
DOI: 10.1111/ijpo.12872 |
|
PMID: 34881510 |
Sample: serum |
DOI: 10.1111/odi.14409 |
|
Sample: hPDLSCs |
PMID: 33469792 |
Sample: Plasma |
PMID: 32508639 |
Sample: Tissue homogenate |
PMID: 33384597 |
Sample: plasma |
DOI: 10.2147/IJN.S134897 |
|
Sample: Cell culture supernatant,Serum |
DOI: 10.1002/ddr.21864 |
|
PMID: 34368980 |
Sample: serum |
PMID: 34737112 |
Sample: MH7A cell |
DOI: 10.1111/jre.12987 |
|
PMID: 35426130 |
Sample: Human gingival fibroblasts (hGFs) |
DOI: 10.3390/nu12103064 |
|
PMID: 33036498 |
Sample: Cell culture supernatant |
Sample: keratinocytes |
PMID: 35897358 |
Sample: serum |
Sample: NHEK cell |
Sample: Caco-2 cells |
DOI: 10.3390/biom11081181 |
|
PMID: 34439847 |
Sample: HaCaT keratinocytes |
DOI: 10.1111/aji.13096 |
|
Sample: Serum |
Sample: serum |
DOI: 10.2147/DMSO.S319863 |
|
PMID: 34345175 |
Sample: serum |
Sample: Cell culture supernatant |
PMID: 30550887 |
Sample: Cell culture supernatant |
PMID: 35687545 |
Sample: serum |
PMID: 35236245 |
Sample: HUVECs |
PMID: 34607526 |
Sample: Human nasal epithelial cells (hNECs) |
DOI: 10.1039/C7FO00469A |
|
Sample: Caco-2 cells |
Sample: THP-1 cells |
DOI: 10.1002/jcb.28724 |
|
Sample: rheumatoid arthritis (RA) fibroblast-like synovioc |
PMID: 35279426 |
Sample: cerebrospinal fluid (CSF) |
PMID: 33521454 |
Sample: Cell culture supernatant |
Sample: SH-SY5Y cell |
Sample: Serum,Cell culture supernatant |
DOI: 10.21037/atm-21-6501 |
|
Sample: A549 cell |
DOI: 10.21037/atm-21-6812 |
|
Sample: adipose-derived stem cell (ASCs) |
PMID: 33182037 |
Sample: Cell lysate |
DOI: 10.1111/nmo.14512 |
|
Sample: serum |
Sample: Saliva |
Sample: Cell culture supernatant |
Sample: Cell culture supernatant |
Sample: Caco-2 cell |
PMID: 30600742 |
Sample: Cell culture supernatant |
Sample: Cell culture supernatant |
Sample: Serum |
PMID: 35331286 |
Sample: chondrocytes |
DOI: 10.1093/mmy/myw066 |
|
Sample: Human L-132 cell,human monocyte cell line (U937),P |
Sample: blood |
PMID: 34194555 |
Sample: Oral cancer tissue |
PMID: 30395811 |
Sample: Serum |
DOI: 10.1042/BSR20181346 |
|
PMID: 30635366 |
Sample: Tissue homogenate |
DOI: 10.1111/crj.13334 |
|
PMID: 33484111 |
Sample: Whole Blood,Serum |
PMID: 31493501 |
Sample: Cell culture supernatant |
DOI: 10.21037/apm-21-2168 |
|
Sample: blood |
DOI: 10.21037/apm-21-1056 |
|
Sample: serum |
Sample: serum |
DOI: 10.1155/2022/9092311 |
|
Sample: Human nasal epithelial cell (hNECs) |
DOI: 10.3892/or.2016.5216 |
|
Sample: macrophage |
DOI: 10.1039/C6FO00831C |
|
Sample: Plasma,Serum |
PMID: 35866915 |
Sample: Ker-CT cell,HaCaT cell |
Sample: human retinal endothelial cell |
Sample: Serum |
Sample: serum |
PMID: 34139308 |
Sample: HNECs cells |
PMID: 32805637 |
Sample: Cell culture supernatant |
Sample: serum |