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QuicKey Pro Human POSTN/OSF-2(Periostin) ELISA Kit

Citations(7)Uniprot : Q15063

Cat.No.:E-OSEL-H0013
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T/24
Assay time	1.5h
Detection range	3.12-200 ng/mL
Sensitivity	1.52 ng/mL
Sample type &Sample volume	serum and plasma; 50μL
Specificity	This kit recognizes POSTN/OSF-2 in samples. No significant cross-reactivity or interference between POSTN/OSF-2 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of POSTN/OSF-2 concentrations in serum and plasma.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human POSTN/OSF-2. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human POSTN/OSF-2 are added to the micro ELISA plate wells. Human POSTN/OSF-2 in samples (or standards) combines with the coated antibody and HRP linked detection antibody specific to POSTN/OSF-2. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Human POSTN/OSF-2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the table.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips 24T: 8 wells ×3 strips 96T*5: 5 plates, 96T	2-8℃, 1 months
Reference Standard	96T: 2 vials 48T/24T: 1 vial 96T*5: 10 vials	2-8℃, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100×)	96T: 1 vial, 60 μL 48T/24T: 1 vial, 30 μL 96T*5: 5 vials, 60 μL	2-8℃(Protect from light)
Reference Standard & Sample Diluent	96T/48T/24T: 1 vial, 20 mL 96T*5: 5 vials, 20 mL	2-8℃
HRP Linked Ab Diluent	96T/48T/24T: 1 vial, 14 mL 96T*5: 5 vials, 14 mL
Concentrated Wash Buffer(25×)	96T/48T/24T: 1 vial, 30 mL 96T*5: 5 vials, 30 mL
Substrate Reagent	96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL	2-8℃(Protect from light)
Stop Solution	96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL	2-8℃
Plate Sealer	96T/48T/24T: 5 pieces 96T*5: 25 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level POSTN/OSF-2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level POSTN/OSF-2 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	1.99	6.27	13.47	2.17	6.59	13.68
Standard deviation	0.13	0.32	0.62	0.12	0.35	0.42
CV (%)	6.78	5.17	4.61	5.58	5.32	3.04

Recovery

The recovery of POSTN/OSF-2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	91-103	98
EDTA plasma(n=8)	84-99	91

Linearity

Samples were spiked with high concentrations of Human POSTN/OSF-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: Q15063
Synonyms	POSTN/OSF-2
Research Area	Cancer, Cardiovascular, Signal transduction, Tags and Cell Markers

Assay Procedures

Citations

Clinical Oral Investigations (2022) IF: 3.606

Evaluation of IL-23p19/Ebi3 (IL-39) gingival crevicular fluid levels in periodontal health, gingivitis, and periodontitis

DOI: 10.1007/s00784-022-04681-w
PMID: 35986791	Sample: gingival crevicular fluid (GCF)

ENDOCRINE (2022) IF: 3.633

Assessment of circulating fibrotic proteins (periostin and tenascin -C) In Type 2 diabetes mellitus patients with and without retinopathy

DOI: 10.1007/s12020-022-03027-6
PMID: 35274283	Sample: serum

INTERNATIONAL JOURNAL OF CLINICAL PRACTICE (2021) IF: 2.503

Serum periostin levels in COVID-19: Is it useful as a new biomarker?

DOI: 10.1111/ijcp.14728
	Sample: serum

Paediatrics and International Child Health (2019) IF: 1.362

Serum periostin levels in acute rheumatic fever: is it useful as a new biomarker?

DOI: 10.1080/20469047.2019.1682330
	Sample: serum

Clinical and Experimental Nephrology (2019) IF: 1.971

E-cadherin and periostin in early detection and progression of diabetic nephropathy: epithelial-to-mesenchymal transition

DOI: 10.1007/s10157-019-01744-3
PMID: 31104272	Sample: Plasma

Clinical Epidemiology and Global Health (2021)

Predictor for cardiovascular risk in patients with type-2 diabetes mellitus

DOI: 10.1016/j.cegh.2021.100870
	Sample: serum

Turkish Journal of Biochemistry-Turk Biyokimya Dergisi (2022) IF: 0.481

Direct-acting antiviral therapy may help restore HCV-induced impaired redox balance and liver fibrosis process

DOI: 10.1515/tjb-2022-0081
	Sample: serum

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