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QuicKey Pro Human POSTN/OSF-2(Periostin) ELISA Kit

  • Cat.No.:E-OSEL-H0013

  • Reactivity: Human

To Purchase E-OSEL-H0013

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $520
Qty:

QuicKey Pro Series

Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T/24
Assay time 1.5h
Detection range 3.12-200 ng/mL
Sensitivity 1.52 ng/mL
Sample type &Sample volume serum and plasma; 50μL
Specificity This kit recognizes POSTN/OSF-2 in samples. No significant cross-reactivity or interference between POSTN/OSF-2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of POSTN/OSF-2 concentrations in serum and plasma.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human POSTN/OSF-2. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human POSTN/OSF-2 are added to the micro ELISA plate wells. Human POSTN/OSF-2 in samples (or standards) combines with the coated antibody and HRP linked detection antibody specific to POSTN/OSF-2. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Human POSTN/OSF-2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the table.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
24T: 8 wells ×3 strips
96T*5: 5 plates, 96T
2-8℃, 1 months
Reference Standard 96T: 2 vials
48T/24T: 1 vial
96T*5: 10 vials
2-8℃, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100×) 96T: 1 vial, 60 μL
48T/24T: 1 vial, 30 μL
96T*5: 5 vials, 60 μL
2-8℃(Protect from light)
Reference Standard & Sample Diluent 96T/48T/24T: 1 vial, 20 mL
96T*5: 5 vials, 20 mL
2-8℃
HRP Linked Ab Diluent 96T/48T/24T: 1 vial, 14 mL
96T*5: 5 vials, 14 mL
Concentrated Wash Buffer(25×) 96T/48T/24T: 1 vial, 30 mL
96T*5: 5 vials, 30 mL
Substrate Reagent 96T/48T/24T: 1 vial, 10 mL
96T*5: 5 vials, 10 mL
2-8℃(Protect from light)
Stop Solution 96T/48T/24T: 1 vial, 10 mL
96T*5: 5 vials, 10 mL
2-8℃
Plate Sealer 96T/48T/24T: 5 pieces
96T*5: 25 pieces
Product Description 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level POSTN/OSF-2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level POSTN/OSF-2 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 1.99 6.27 13.47 2.17 6.59 13.68
Standard deviation 0.13 0.32 0.62 0.12 0.35 0.42
CV (%) 6.78 5.17 4.61 5.58 5.32 3.04

Recovery

The recovery of POSTN/OSF-2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-103 98
EDTA plasma(n=8) 84-99 91

Linearity

Samples were spiked with high concentrations of Human POSTN/OSF-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: Q15063
SynonymsPOSTN/OSF-2
Research AreaCancer, Cardiovascular, Signal transduction, Tags and Cell Markers

Assay Procedures

Citations

  1. Clinical Epidemiology and Global Health (2021)
    Predictor for cardiovascular risk in patients with type-2 diabetes mellitus

    DOI: 10.1016/j.cegh.2021.100870

    Sample: Serum
  2. Turkish Journal of Biochemistry-Turk Biyokimya Dergisi (2022) IF: 0.481
    Direct-acting antiviral therapy may help restore HCV-induced impaired redox balance and liver fibrosis process

    DOI: 10.1515/tjb-2022-0081

    Sample: Serum
  3. Paediatrics and International Child Health (2019) IF: 1.362
    Serum periostin levels in acute rheumatic fever: is it useful as a new biomarker?

    DOI: 10.1080/20469047.2019.1682330

    Sample: Serum
  4. Clinical and Experimental Nephrology (2019) IF: 1.971
    E-cadherin and periostin in early detection and progression of diabetic nephropathy: epithelial-to-mesenchymal transition

    DOI: 10.1007/s10157-019-01744-3

    PMID: 31104272

    Sample: Plasma
  5. INTERNATIONAL JOURNAL OF CLINICAL PRACTICE (2021) IF: 2.503
    Serum periostin levels in COVID-19: Is it useful as a new biomarker?

    DOI: 10.1111/ijcp.14728

    Sample: Serum
  6. Clinical Oral Investigations (2022) IF: 3.606
    Evaluation of IL-23p19/Ebi3 (IL-39) gingival crevicular fluid levels in periodontal health, gingivitis, and periodontitis

    DOI: 10.1007/s00784-022-04681-w

    PMID: 35986791

    Sample: Gingival Crevicular Fluid (Gcf)
  7. ENDOCRINE (2022) IF: 3.633
    Assessment of circulating fibrotic proteins (periostin and tenascin -C) In Type 2 diabetes mellitus patients with and without retinopathy

    DOI: 10.1007/s12020-022-03027-6

    PMID: 35274283

    Sample: Serum
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