QuicKey Pro Mouse CORT(Corticosterone) ELISA Kit (E-OSEL-M0001)

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse Cort. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse Cort are added to the micro ELISA plate wells. Mouse Cort in samples (or standards) competes with a fixed amount of Cort on the solid phase supporter for sites on the HRP linked detection antibody specific to Cort. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Mouse Cort in the samples is then determined by comparing the OD of the samples to the standard curve.

Corticosterone (CORT) administration in drinking water has been widely used by researchers. CORT is a principal GC in rodents because their adrenals do not express 17α-hydroxylase and 17, 20-lyase (CYP17A1), which are key enzymes in the synthesis of both cortisol and adrenal androgens. As CORT is hydrophobic, it must be dissolved in 100% ethanol and then diluted with water to the suitable CORT concentration with 1% ethanol content.