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Cat.No.:E-OSEL-M0001
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Assay type | Competitive-ELISA |
Format | 96T/48T/24 |
Assay time | 1.5h |
Detection range | 12.50-800 ng/mL |
Sensitivity | 4.98 ng/mL |
Sample type &Sample volume | serum, plasma, urine, saliva; 50μL |
Specificity | This kit recognizes Cort in samples. No significant cross-reactivity or interference between Cort and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of Cort concentrations in serum, plasma, urine, saliva. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 24T: 8 wells ×3 strips 96T*5: 5 plates, 96T |
2-8℃, 1 months |
Reference Standard | 96T: 2 vials
48T/24T: 1 vial 96T*5: 10 vials |
2-8℃, use the reconstituted standard within 24h |
Concentrated HRP Linked Detection Ab(100×) | 96T: 1 vial, 60 μL
48T/24T: 1 vial, 30 μL 96T*5: 5 vials, 60 μL |
2-8℃(Protect from light) |
Reference Standard & Sample Diluent | 96T/48T/24T: 1 vial, 20 mL 96T*5: 5 vials, 20 mL |
2-8℃ |
HRP Linked Ab Diluent | 96T/48T/24T: 1 vial, 14 mL 96T*5: 5 vials, 14 mL |
|
Concentrated Wash Buffer(25×) | 96T/48T/24T: 1 vial, 30 mL 96T*5: 5 vials, 30 mL |
|
Substrate Reagent | 96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL |
2-8℃(Protect from light) |
Stop Solution | 96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL |
2-8℃ |
Plate Sealer | 96T/48T/24T: 5 pieces 96T*5: 25 pieces |
|
Product Description | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Cort were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Cort were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (ng/mL) | 41.41 | 114.40 | 374.35 | 42.38 | 108.31 | 346.98 |
Standard deviation | 2.77 | 5.70 | 20.03 | 2.95 | 5.10 | 18.08 |
CV (%) | 6.69 | 4.98 | 5.35 | 6.95 | 4.71 | 5.21 |
The recovery of Cort spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 91-106 | 98 |
EDTA plasma(n=8) | 88-103 | 94 |
Samples were spiked with high concentrations of Mouse Cort and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Synonyms | Corticosterone,CORT |
Research Area | Cell biology |
Sample: Lung |
PMID: 35863473 |
Sample: Serum |
PMID: 32778988 |
Sample: Serum |
PMID: 34129201 |
Sample: Serum |
Sample: Plasma |
DOI: 10.3920/BM2016.0226 |
|
Sample: Serum,Cell Culture Supernatant |
Sample: Plasma |
DOI: 10.1002/mrd.23515 |
|
PMID: 34032349 |
Sample: Serum |
PMID: 33296408 |
Sample: Serum |
PMID: 29452193 |
Sample: Serum |
Sample: Plasma |
PMID: 32853749 |
Sample: Serum |
DOI: 10.3390/nu11040819 |
|
Sample: Blood |
Sample: Serum |
Sample: Tissue Homogenate |
PMID: 33731795 |
Sample: Plasma |
Sample: Plasma |
PMID: 33310695 |
Sample: Serum |
PMID: 34768069 |
Sample: Serum |
Sample: Plasma |
DOI: 10.1039/D2FO01164F |
|
PMID: 35763064 |
Sample: Serum |
DOI: 10.1039/D1FO01608C |
|
PMID: 34704999 |
Sample: Serum |
PMID: 34391441 |
Sample: Blood |
DOI: 10.1155/2022/6877283 |
|
PMID: 35707281 |
Sample: Serum |
Sample: Serum |
PMID: 36669474 |
Sample: Serum |