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Current Position:Home>>Hot>>QuicKey Pro ELISA>> QuicKey Pro Mouse CORT(Corticosterone) ELISA Kit

QuicKey Pro Mouse CORT(Corticosterone) ELISA Kit

Citations(26)

  • Cat.No.:E-OSEL-M0001

  • Reactivity: Mouse

To Purchase E-OSEL-M0001

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $520
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QuicKey Pro Series

Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Product Details

Properties

Assay type Competitive-ELISA
Format 96T/48T/24
Assay time 1.5h
Detection range 12.50-800 ng/mL
Sensitivity 4.98 ng/mL
Sample type &Sample volume serum, plasma, urine, saliva; 50μL
Specificity This kit recognizes Cort in samples. No significant cross-reactivity or interference between Cort and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Cort concentrations in serum, plasma, urine, saliva.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse Cort. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse Cort are added to the micro ELISA plate wells. Mouse Cort in samples (or standards) competes with a fixed amount of Cort on the solid phase supporter for sites on the HRP linked detection antibody specific to Cort. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Mouse Cort in the samples is then determined by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the table.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
24T: 8 wells ×3 strips
96T*5: 5 plates, 96T 		2-8℃, 1 months
Reference Standard 96T: 2 vials
48T/24T: 1 vial
96T*5: 10 vials 		2-8℃, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100×) 96T: 1 vial, 60 μL
48T/24T: 1 vial, 30 μL
96T*5: 5 vials, 60 μL 		2-8℃(Protect from light)
Reference Standard & Sample Diluent 96T/48T/24T: 1 vial, 20 mL
96T*5: 5 vials, 20 mL		 2-8℃
HRP Linked Ab Diluent 96T/48T/24T: 1 vial, 14 mL
96T*5: 5 vials, 14 mL
Concentrated Wash Buffer(25×) 96T/48T/24T: 1 vial, 30 mL
96T*5: 5 vials, 30 mL
Substrate Reagent 96T/48T/24T: 1 vial, 10 mL
96T*5: 5 vials, 10 mL		 2-8℃(Protect from light)
Stop Solution 96T/48T/24T: 1 vial, 10 mL
96T*5: 5 vials, 10 mL		 2-8℃
Plate Sealer 96T/48T/24T: 5 pieces
96T*5: 25 pieces
Product Description 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Cort were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Cort were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 41.41 114.40 374.35 42.38 108.31 346.98
Standard deviation 2.77 5.70 20.03 2.95 5.10 18.08
CV (%) 6.69 4.98 5.35 6.95 4.71 5.21

Recovery

The recovery of Cort spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-106 98
EDTA plasma(n=8) 88-103 94

Linearity

Samples were spiked with high concentrations of Mouse Cort and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsCorticosterone，CORT
Research AreaCell biology

Assay Procedures

Citations

  1. CELL (2023) IF: 66.849
    Light modulates glucose metabolism by a retina-hypothalamus-brown adipose tissue axis

    DOI: 10.1016/j.cell.2022.12.024

    PMID: 36669474

    		 Sample: Serum
  2. Neurobiology of Stress (2022) IF: 7.142
    The antidepressant effect of nucleus accumbens deep brain stimulation is mediated by parvalbumin-positive interneurons in the dorsal dentate gyrus

    DOI: 10.1016/j.ynstr.2022.100492
    Sample: serum
  3. CHINESE JOURNAL OF CHEMISTRY (2021) IF: 6
    Uncarialins J‐M from Uncaria rhynchophylla and their anti‐depression mechanism in unpredictable chronic mild stress‐induced mice via activating 5‐HT1A receptor

    DOI: 10.1002/cjoc.202000652
    Sample: lung
  4. Oxidative Medicine and Cellular Longevity (2022) IF: 6.543
    Small Noncoding RNAs Contribute to Sperm Oxidative Stress-Induced Programming of Behavioral and Metabolic Phenotypes in Offspring

    DOI: 10.1155/2022/6877283

    PMID: 35707281

    		 Sample: serum
  5. CHEMICO-BIOLOGICAL INTERACTIONS (2022) IF: 5.168
    Binge alcohol consumption exacerbates high-fat diet-induced neurobehavioral anomalies: Possible underlying mechanisms

    DOI: 10.1016/j.cbi.2022.110039

    PMID: 35863473

    		 Sample: serum
  6. MOLECULAR NEUROBIOLOGY (2014) IF: 5.286
    Olfactory Deprivation Hastens Alzheimer-Like Pathologies in a Human Tau-Overexpressed Mouse Model via Activation of cdk5

    DOI: 10.1007/s12035-014-9007-z
    Sample: Plasma
  7. Food & Function (2022) IF: 5.396
    Perinatal Transmission of a Probiotic Bifidobacterium Strain Protects Against Early Life Stress-induced Mood and Gastrointestinal Motility Disorders

    DOI: 10.1039/D2FO01164F

    PMID: 35763064

    		 Sample: serum
  8. Food & Function (2021) IF: 5.396
    Pediococcus acidilactici CCFM6432 mitigates chronic stress-induced anxiety and gut microbial abnormalities

    DOI: 10.1039/D1FO01608C

    PMID: 34704999

    		 Sample: serum
  9. Chinese Medicine (2021) IF: 5.455
    Chaihu-Shugan-San (Shihosogansan) alleviates restraint stress-generated anxiety and depression in mice by regulating NF-κB-mediated BDNF expression through the modulation of gut microbiota

    DOI: 10.1186/s13020-021-00492-5

    PMID: 34391441

    		 Sample: blood
  10. Nutrients (2019) IF: 4.171
    The Preventive and Curative Effects of Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98 on Immobilization Stress-Induced Anxiety/Depression and Colitis in Mice

    DOI: 10.3390/nu11040819
    Sample: blood
  11. PHYTOMEDICINE (2020) IF: 4.268
    Uncaria rhynchophylla ameliorates unpredictable chronic mild stress-induced depression in mice via activating 5-HT1A receptor: Insights from transcriptomics

    DOI: 10.1016/j.phymed.2020.153436
    Sample: serum
  12. Frontiers in Cellular and Infection Microbiology (2017) IF: 4.3
    Microbiota Modulate Anxiety-Like Behavior and Endocrine Abnormalities in Hypothalamic-Pituitary-Adrenal Axis

    DOI: 10.3389/fcimb.2017.00489
    Sample: Tissue homogenate
  13. Scientific Reports (2021) IF: 4.38
    Buspirone alleviates anxiety, depression, and colitis; and modulates gut microbiota in mice

    DOI: 10.1038/s41598-021-85681-w

    PMID: 33731795

    		 Sample: Plasma
  14. BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
    Antidepressant and anti-amnesic effects of the aqueous lyophilisate of the leaves of Leptadenia arborea on an animal model of cognitive deficit associated depression

    DOI: 10.1016/j.biopha.2020.110603
    Sample: plasma
  15. PSYCHONEUROENDOCRINOLOGY (2020) IF: 4.732
    Differential modulation of GR signaling and HDACs in the development of resilient/vulnerable phenotype and antidepressant-like response of vorinostat

    DOI: 10.1016/j.psyneuen.2020.105083

    PMID: 33310695

    		 Sample: serum
  16. JOURNAL OF PSYCHIATRIC RESEARCH (2021) IF: 4.791
    Lipopolysaccharide exacerbates chronic restraint stress-induced neurobehavioral deficits: Mechanisms by redox imbalance, ASK1-related apoptosis, autophagic dysregulation

    DOI: 10.1016/j.jpsychires.2021.10.021

    PMID: 34768069

    		 Sample: serum
  17. BEHAVIOURAL BRAIN RESEARCH (2018) IF: 3.173
    Antidepressant activity of vorinostat is associated with amelioration of oxidative stress and inflammation in a corticosterone-induced chronic stress model in mice

    DOI: 10.1016/j.bbr.2018.02.009

    PMID: 29452193

    		 Sample: Serum
  18. HORMONES AND BEHAVIOR (2017) IF: 3.378
    What is stressful for females? Differential effects of unpredictable environmental or social stress in CD1 female mice

    DOI: 10.1016/j.yhbeh.2017.11.013
    Sample: plasma
  19. NEUROCHEMISTRY INTERNATIONAL (2020) IF: 3.881
    Hesperidin alleviates chronic restraint stress and lipopolysaccharide-induced Hippocampus and Frontal cortex damage in mice: Role of TLR4/NF-κB, p38 MAPK/JNK, Nrf2/ARE signaling

    DOI: 10.1016/j.neuint.2020.104835

    PMID: 32853749

    		 Sample: Serum
  20. Current Medical Science (2021) IF: 2.135
    Protective Effect of Hydroxysafflor Yellow A against Chronic Mild Stress-induced Memory Impairments by Suppressing Tau Phosphorylation in Mice

    DOI: 10.1007/s11596-021-2369-3

    PMID: 34129201

    		 Sample: serum
  21. METABOLIC BRAIN DISEASE (2017) IF: 2.297
    Combined corticosterone treatment and chronic restraint stress lead to depression associated with early cognitive deficits in mice

    DOI: 10.1007/s11011-017-0148-4
    Sample: Plasma
  22. Beneficial Microbes (2018) IF: 2.31
    Anxiolytic-like effect of Bifidobacterium adolescentis IM38 in mice with or without immobilisation stress

    DOI: 10.3920/BM2016.0226
    Sample: Serum,Cell culture supernatant
  23. JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2018) IF: 2.478
    High activity of the endogenous opioid system and acute but not chronic stress influence experimental colitis development in mice

    DOI: 10.26402/jpp.2018.5.11
    Sample: plasma
  24. MOLECULAR REPRODUCTION AND DEVELOPMENT (2021) IF: 2.609
    Dysfunction in Sertoli cells participates in glucocorticoid-induced impairment of spermatogenesis

    DOI: 10.1002/mrd.23515

    PMID: 34032349

    		 Sample: Serum
  25. PLoS One (2020) IF: 2.74
    Hypergravity as a gravitational therapy mitigates the effects of knee osteoarthritis on the musculoskeletal system in a murine model

    DOI: 10.1371/journal.pone.0243098

    PMID: 33296408

    		 Sample: Serum
  26. MOLECULAR BIOLOGY REPORTS (2020) IF: 1.402
    The role of hippocampal GABA A receptors on anxiolytic effects of Echium amoenum extract in a mice model of restraint stress

    DOI: 10.1007/s11033-020-05699-7

    PMID: 32778988

    		 Sample: Serum
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