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QuicKey Pro Mouse E2(Estradiol) ELISA Kit

Citations(34)

  • Cat.No.:E-OSEL-M0008

  • Reactivity: Mouse
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Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $520
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QuicKey Pro Series

Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Product Details

Properties

Assay type Competitive-ELISA
Format 96T/48T/24
Assay time 1.5h
Detection range 3.13-200 pg/mL
Sensitivity 1.88 pg/mL
Sample type &Sample volume Serum, Plasma, Urine, Saliva; 50μL
Specificity This kit recognizes E2 in samples. No significant cross-reactivity or interference between E2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of E2 concentrations in Serum, Plasma, Urine, Saliva.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse E2. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse E2 are added to the micro ELISA plate wells. Mouse E2 in samples (or standards) competes with a fixed amount of E2 on the solid phase supporter for sites on the HRP linked detection antibody specific to E2. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Mouse E2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the table.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
24T: 8 wells ×3 strips
96T*5: 5 plates, 96T 		2-8℃, 1 months
Reference Standard 96T: 2 vials
48T/24T: 1 vial
96T*5: 10 vials 		2-8℃, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100×) 96T: 1 vial, 60 μL
48T/24T: 1 vial, 30 μL
96T*5: 5 vials, 60 μL 		2-8℃(Protect from light)
Reference Standard & Sample Diluent 96T/48T/24T: 1 vial, 20 mL
96T*5: 5 vials, 20 mL		 2-8℃
HRP Linked Ab Diluent 96T/48T/24T: 1 vial, 14 mL
96T*5: 5 vials, 14 mL
Concentrated Wash Buffer(25×) 96T/48T/24T: 1 vial, 30 mL
96T*5: 5 vials, 30 mL
Substrate Reagent 96T/48T/24T: 1 vial, 10 mL
96T*5: 5 vials, 10 mL		 2-8℃(Protect from light)
Stop Solution 96T/48T/24T: 1 vial, 10 mL
96T*5: 5 vials, 10 mL		 2-8℃
Plate Sealer 96T/48T/24T: 5 pieces
96T*5: 25 pieces
Product Description 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level E2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level E2 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 10.42 23.40 67.53 11.05 22.49 72.44
Standard deviation 0.65 1.36 3.14 0.74 1.09 3.58
CV (%) 6.26 5.80 4.65 6.72 4.83 4.94

Recovery

The recovery of E2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-102 97
EDTA plasma(n=8) 89-102 96
Cell culture media(n=8) 88-101 95

Linearity

Samples were spiked with high concentrations of Mouse E2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsOestradiol, E2, 17β-Estradiol, Estra-1,3,5(10)-triene-3,17β-diol
Research AreaCancer, Cardiovascular, Metabolism, Neuroscience, Signaling transduction

Assay Procedures

Citations

  1. JOURNAL OF HAZARDOUS MATERIALS (2021) IF: 10.588
    Di (2-ethyl-hexyl) phthalate disrupts placental growth in a dual blocking mode

    DOI: 10.1016/j.jhazmat.2021.126815
    Sample: serum
  2. ENVIRONMENTAL POLLUTION (2022) IF: 9.988
    Betulinic acid alleviates zearalenone-induced uterine injury in mice

    DOI: 10.1016/j.envpol.2022.120435

    PMID: 36257561

    		 Sample: serum
  3. ENVIRONMENTAL POLLUTION (2021) IF: 8.071
    Tannic acid repair of zearalenone-induced damage by regulating the death receptor and mitochondrial apoptosis signaling pathway in mice

    DOI: 10.1016/j.envpol.2021.117557

    PMID: 34167001

    		 Sample: serum
  4. Cell Death & Disease (2021) IF: 8.4689
    MeCP2 duplication causes hyperandrogenism by upregulating LHCGR and downregulating RORα

    DOI: 10.1038/s41419-021-04277-4

    PMID: 34697294

    		 Sample: serum
  5. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2022) IF: 7.129
    Maternal exposure to cadmium from puberty through lactation induces abnormal reproductive development in female offspring

    DOI: 10.1016/j.ecoenv.2022.113927

    PMID: 35908533

    		 Sample: serum
  6. ENVIRONMENT INTERNATIONAL (2020) IF: 7.577
    Bisphenol A promotes autophagy in ovarian granulosa cells by inducing AMPK/mTOR/ULK1 signalling pathway

    DOI: 10.1016/j.envint.2020.106298

    PMID: 33387880

    		 Sample: Cell culture supernatant,Serum
  7. Food Science and Human Wellness (2022) IF: 5.154
    The protective effects of Procyanidin C-1 on bisphenol a-induced testicular dysfunction in aged mice

    DOI: 10.1016/j.fshw.2022.03.020
    Sample: serum
  8. Reproductive Biology and Endocrinology (2021) IF: 5.211
    Irisin deletion induces a decrease in growth and fertility in mice

    DOI: 10.1186/s12958-021-00702-7

    PMID: 33581723

    		 Sample: Serum
  9. Scientific Reports (2016) IF: 5.228
    Knockout of BRD7 results in impaired spermatogenesis and male infertility

    DOI: 10.1038/srep21776
    Sample: serum
  10. Food & Function (2022) IF: 5.396
    Tilapia skin peptides restore cyclophosphamide-induced premature ovarian failure via inhibiting oxidative stress and apoptosis in mice

    DOI: 10.1039/D1FO04239D

    PMID: 35083997

    		 Sample: serum
  11. Aging-US (2021) IF: 5.682
    Fecal microbiota transplantation mitigates vaginal atrophy in ovariectomized mice

    DOI: 10.18632/aging.202627

    PMID: 33658399

    		 Sample: Serum
  12. ENVIRONMENTAL POLLUTION (2019) IF: 5.714
    Exposure to DEHP or its metabolite MEHP promotes progesterone secretion and inhibits proliferation in mouse placenta or JEG-3 cells

    DOI: 10.1016/j.envpol.2019.113593
    Sample: Cell culture medium
  13. Nanotoxicology (2019) IF: 5.955
    Metabolomics reveals the role of acetyl-l-carnitine metabolism in γ-Fe2O3 NP-induced embryonic development toxicity via mitochondria damage

    DOI: 10.1080/17435390.2018.1537411
    Sample: Serum
  14. JOURNAL OF REPRODUCTIVE IMMUNOLOGY (2020) IF: 4.018
    Ovalbumin causes impairment of preimplantation embryonic growth in asthma-induced mice

    DOI: 10.1016/j.jri.2020.103240

    PMID: 33166807

    		 Sample: Serum
  15. Scientific Reports (2017) IF: 4.259
    Diet-induced obesity impairs spermatogenesis: a potential role for autophagy

    DOI: 10.1038/srep43475
    Sample: Serum
  16. BIOLOGY OF REPRODUCTION (2022) IF: 4.285
    Pre-implantation triclosan exposure alters uterine receptivity through affecting tight junction protein.

    DOI: 10.1093/biolre/ioac092
    Sample: serum
  17. BIOLOGY OF REPRODUCTION (2022) IF: 4.285
    Loss of raptor induces Sertoli cells into an undifferentiated state in mice

    DOI: 10.1093/biolre/ioac104

    PMID: 35594452

    		 Sample: gonads
  18. ENVIRONMENTAL POLLUTION (2018) IF: 4.358
    Neonatal triphenyl phosphate and its metabolite diphenyl phosphate exposure induce sex- and dose-dependent metabolic disruptions in adult mice

    DOI: 10.1016/j.envpol.2018.01.047
    Sample: serum
  19. JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36
    The effect of Bu Shen Huo Xue Tang on autoimmune premature ovarian insufficiency via Modulation of the Nrf2/Keap1 signaling pathway in mice

    DOI: 10.1016/j.jep.2021.113996

    PMID: 33684516

    		 Sample: Serum
  20. JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36
    Anti-perimenopausal osteoporosis effects of Erzhi formula via regulation of bone resorption through osteoclast differentiation: A network pharmacology-integrated experimental study

    DOI: 10.1016/j.jep.2021.113815

    PMID: 33444724

    		 Sample: Plasma
  21. Human Cell (2022) IF: 4.374
    Repeated controlled ovarian stimulation-induced ovarian and uterine damage in mice through the PI3K/AKT signaling pathway

    DOI: 10.1007/s13577-022-00829-8
    Sample: serum
  22. Scientific Reports (2022) IF: 4.996
    Effects of Cordyceps militaris fermentation products on reproductive development in juvenile male mice

    DOI: 10.1038/s41598-022-18066-2

    PMID: 35962055

    		 Sample: serum
  23. JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2020) IF: 3.606
    Protective roles and mechanisms of rosmarinic acid in cyclophosphamide‐induced premature ovarian failure

    DOI: 10.1002/jbt.22591
    Sample: Serum
  24. CHEMOSPHERE (2016) IF: 3.698
    Disruption of sex-hormone levels and steroidogenic-related gene expression on Mongolia Racerunner (Eremias argus) after exposure to triadimefon and its enantiomers

    DOI: 10.1016/j.chemosphere.2016.12.096
    Sample: Plasma
  25. AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY (2023) IF: 3.777
    Hydroxychloroquine protects against autoimmune premature ovarian insufficiency by modulating the Treg/Th17 cell ratio in BALB/c mice

    DOI: 10.1111/aji.13686
    Sample: blood
  26. JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2019) IF: 2.422
    Oral oyster polypeptides protect ovary against d‐galactose‐induced premature ovarian failure in C57BL/6 mice

    DOI: 10.1002/jsfa.9997
    Sample: Serum
  27. MOLECULAR REPRODUCTION AND DEVELOPMENT (2020) IF: 2.609
    Adoptive transfers of CD4+CD25+ Tregs partially alleviate mouse premature ovarian insufficiency

    DOI: 10.1002/mrd.23404
    Sample: Serum
  28. Evidence-based Complementary and Alternative Medicine (2021) IF: 2.63
    Effect of Electroacupuncture on Reproductive Disorders and Insulin Resistance in a Murine Polycystic Ovary Syndrome Model

    DOI: 10.1155/2021/9968463

    PMID: 34987599

    		 Sample: serum
  29. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL (2022) IF: 2.723
    Cytosine-phosphate-guanine oligodeoxynucleotides regulate the cell cycle, apoptosis, and steroidogenesis of mouse ovarian granulosa cells by targeting inhibin alpha (1 ~ 32) fragments

    DOI: 10.1007/s11626-022-00662-1

    PMID: 35378691

    		 Sample: mGCs
  30. PLoS One (2020) IF: 2.74
    Effect of ovarian stimulation on the expression of piRNA pathway proteins

    DOI: 10.1371/journal.pone.0232629
    Sample: plasma
  31. Reproductive Biology and Endocrinology (2018) IF: 2.852
    Alleviation of endoplasmic reticulum stress protects against cisplatin-induced ovarian damage

    DOI: 10.1186/s12958-018-0404-4

    PMID: 30176887

    		 Sample: Plasma
  32. Analytical Cellular Pathology (2021) IF: 2.916
    Establishment and Mechanism Study of a Primary Ovarian Insufficiency Mouse Model Using Lipopolysaccharide

    DOI: 10.1155/2021/1781532
    Sample: serum
  33. Chinese Medicine (2020) IF: 2.96
    Catechins from oolong tea improve uterine defects by inhibiting STAT3 signaling in polycystic ovary syndrome mice

    DOI: 10.1186/s13020-020-00405-y

    PMID: 33292347

    		 Sample: serum
  34. Journal of Ayurveda and Integrative Medicine (2020)
    Hormone-balancing and protective effect of combined extract of Sauropus androgynus and Elephantopus scaber against Escherichia coli-induced renal and hepatic necrosis in pregnant mice

    DOI: 10.1016/j.jaim.2020.09.001
    Sample: Serum
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