QuicKey Pro Mouse E3(Estriol) ELISA Kit (E-OSEL-M0004)
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For research use only.
Product Summary
Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.
| Sensitivity | 5.83 pg/mL |
| Detection Range | 15.63-1000 pg/mL |
| Sample Volume | 50 μL |
| Total Assay Time | 1 h 30 min |
| Reactivity | Mouse |
| Specificity | This kit recognizes Mouse E3 in samples.No significant cross-reactivity or interference between Mouse E3 and analogues was observed |
| Recovery | 80%-120% |
| Sample Type | serum, plasma |
| Detection Method | Colorimetric method, ELISA, Competitive |
| Assay Type | Competitive-ELISA |
| Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
| Storage | 2-8℃ |
| Expiration Date | 6 months |
Test Principle
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse E3. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse E3 are added to the micro ELISA plate wells. Mouse E3 in samples (or standards) competes with a fixed amount of E3 on the solid phase supporter for sites on the HRP linked detection antibody specific to E3. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Mouse E3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Background
Estriol is a sex hormone that can be detected in the urine of pregnant women. The effect of estrogen is weaker than that of estrone. Dhea, which is produced in the adrenal glands of the fetus, is hydroxylated by 16α in the liver and aromatized in the placenta to become estriol. As the pregnancy progresses, the amount of excretion in the urine gradually increases, and then drops sharply after delivery. As a palliative sex hormone reagent, it can treat menopause disorders by oral administration.
| Research Area | Cell Biology , Signal Transduction , Developmental Biology |
| Cat.No. | Product Name | Clone No. |
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