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QuicKey Pro Mouse IgA(Immunoglobulin A) ELISA Kit

Cat.No.:E-OSEL-M0007
Reactivity: Mouse

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96T*5
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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T/24
Assay time	1.5h
Detection range	1.56-100 ng/mL
Sensitivity	0.94 ng/mL
Sample type &Sample volume	serum and plasma; 50μL
Specificity	This kit recognizes IgA in samples. No significant cross-reactivity or interference between IgA and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of IgA concentrations in serum and plasma.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IgA. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse IgA are added to the micro ELISA plate wells. Mouse IgA in samples (or standards) combines with the coated antibody and HRP linked detection antibody specific to IgA. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Mouse IgA in the samples is then determined by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the table.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips 24T: 8 wells ×3 strips 96T*5: 5 plates, 96T	2-8℃, 1 months
Reference Standard	96T: 2 vials 48T/24T: 1 vial 96T*5: 10 vials	2-8℃, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100×)	96T: 1 vial, 60 μL 48T/24T: 1 vial, 30 μL 96T*5: 5 vials, 60 μL	2-8℃(Protect from light)
Reference Standard & Sample Diluent	96T/48T/24T: 1 vial, 20 mL 96T*5: 5 vials, 20 mL	2-8℃
HRP Linked Ab Diluent	96T/48T/24T: 1 vial, 14 mL 96T*5: 5 vials, 14 mL
Concentrated Wash Buffer(25×)	96T/48T/24T: 1 vial, 30 mL 96T*5: 5 vials, 30 mL
Substrate Reagent	96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL	2-8℃(Protect from light)
Stop Solution	96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL	2-8℃
Plate Sealer	96T/48T/24T: 5 pieces 96T*5: 25 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level IgA were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level IgA were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	5.31	10.82	37.83	5.54	11.76	34.39
Standard deviation	0.34	0.51	1.97	0.39	0.70	1.60
CV (%)	6.45	4.67	5.22	6.99	5.94	4.66

Recovery

The recovery of IgA spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	93-104	99
EDTA plasma(n=8)	91-104	98

Linearity

Samples were spiked with high concentrations of Mouse IgA and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	Immunoglobulin A,IgA
Research Area	Cancer, Immunology

Assay Procedures

Citations

International Journal of Nanomedicine (2022) IF: 6.4

The Immune-Enhancing Properties of Hwanglyeonhaedok-Tang-Mediated Biosynthesized Gold Nanoparticles in Macrophages and Splenocytes

DOI: 10.2147/IJN.S338334
PMID: 35125869	Sample: RAW264.7 cell,splenocytes

Scientific Reports (2016) IF: 5.228

Protein Kinase D2 Protects against Acute Colitis Induced by Dextran Sulfate Sodium in Mice

DOI: 10.1038/srep34079
	Sample: Serum

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2022) IF: 4.125

Preventive and synbiotic effects of the soluble dietary fiber obtained from Lentinula edodes byproducts and Lactobacillus plantarum LP90 against dextran sulfate sodium-induced colitis in mice

DOI: 10.1002/jsfa.12173
PMID: 36054505	Sample: serum,colon tissue

Food & Function (2020) IF: 4.171

Melatonin as an inducer of arecoline and their coordinated roles in anti-oxidative activity and immune responses

DOI: 10.1039/D0FO01841D
PMID: 32955540	Sample: Plasma

Foods (2022) IF: 4.35

Lactobacillus fermentum Stimulates Intestinal Secretion of Immunoglobulin A in an Individual-Specific Manner

DOI: 10.3390/foods11091229
PMID: 35563952	Sample: Duodenum,jejunum,ileum,colon,colon contents

Journal of Functional Foods (2022) IF: 4.451

Immune-enhancing efficacy of Curtobacterium proimmune K3 lysates isolated from Panax ginseng beverages in cyclophosphamide-induced immunosuppressed mice

DOI: 10.1016/j.jff.2022.105020
	Sample: serum

Journal of Functional Foods (2020) IF: 3.701

Dietary supplementation of Bacillus sp. DU106 activates innate immunity and regulates intestinal microbiota in mice

DOI: 10.1016/j.jff.2020.104247
	Sample: Serum

MICROBIAL PATHOGENESIS (2019) IF: 2.581

Metabolites of stable fly reduce diarrhea in mice by modulating the immune system, antioxidants, and composition of gut microbiota

DOI: 10.1016/j.micpath.2019.103557
	Sample: serum

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