QuicKey Pro Series

Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Product Details

Properties

Assay type	Competitive-ELISA
Format	96T/48T/24
Assay time	1.5h
Detection range	78.13-5000 pg/mL
Sensitivity	24.97 pg/mL
Sample type &Sample volume	serum and plasma; 50μL
Specificity	This kit recognizes Pg in samples. No significant cross-reactivity or interference between Pg and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Pg concentrations in serum and plasma.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse Pg. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse Pg are added to the micro ELISA plate wells. Mouse Pg in samples (or standards) competes with a fixed amount of Pg on the solid phase supporter for sites on the HRP linked detection antibody specific to Pg. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Mouse Pg in the samples is then determined by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the table.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips 24T: 8 wells ×3 strips 96T*5: 5 plates, 96T	2-8℃, 1 months
Reference Standard	96T: 2 vials 48T/24T: 1 vial 96T*5: 10 vials	2-8℃, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100×)	96T: 1 vial, 60 μL 48T/24T: 1 vial, 30 μL 96T*5: 5 vials, 60 μL	2-8℃(Protect from light)
Reference Standard & Sample Diluent	96T/48T/24T: 1 vial, 20 mL 96T*5: 5 vials, 20 mL	2-8℃
HRP Linked Ab Diluent	96T/48T/24T: 1 vial, 14 mL 96T*5: 5 vials, 14 mL
Concentrated Wash Buffer(25×)	96T/48T/24T: 1 vial, 30 mL 96T*5: 5 vials, 30 mL
Substrate Reagent	96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL	2-8℃(Protect from light)
Stop Solution	96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL	2-8℃
Plate Sealer	96T/48T/24T: 5 pieces 96T*5: 25 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Pg were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Pg were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	272.69	578.76	1903.79	255.63	574.20	1978.04
Standard deviation	17.59	27.09	59.97	17.74	30.83	98.51
CV (%)	6.45	4.68	3.15	6.94	5.37	4.98

Recovery

The recovery of Pg spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	91-104	97
EDTA plasma(n=8)	91-101	96

Linearity

Samples were spiked with high concentrations of Mouse Pg and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	Progesterone，Pg
Research Area	Signal Transduction

Assay Procedures

Citations

Reproductive Biology and Endocrinology (2021) IF: 5.211

Irisin deletion induces a decrease in growth and fertility in mice

DOI: 10.1186/s12958-021-00702-7
PMID: 33581723	Sample: Serum

Journal of Ayurveda and Integrative Medicine (2020)

Hormone-balancing and protective effect of combined extract of Sauropus androgynus and Elephantopus scaber against Escherichia coli-induced renal and hepatic necrosis in pregnant mice

DOI: 10.1016/j.jaim.2020.09.001
	Sample: Serum

GENE (2018) IF: 2.498

Obesity-induced overexpression of miRNA-24 regulates cholesterol uptake and lipid metabolism by targeting SR-B1

DOI: 10.1016/j.gene.2018.05.072
	Sample: Serum

Evidence-based Complementary and Alternative Medicine (2021) IF: 2.63

Effect of Electroacupuncture on Reproductive Disorders and Insulin Resistance in a Murine Polycystic Ovary Syndrome Model

DOI: 10.1155/2021/9968463
PMID: 34987599	Sample: Serum

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL (2022) IF: 2.723

Cytosine-phosphate-guanine oligodeoxynucleotides regulate the cell cycle, apoptosis, and steroidogenesis of mouse ovarian granulosa cells by targeting inhibin alpha (1 ~ 32) fragments

DOI: 10.1007/s11626-022-00662-1
PMID: 35378691	Sample: Mgcs

Reproductive Biology and Endocrinology (2020) IF: 3.235

Genipin improves reproductive health problems caused by circadian disruption in male mice

DOI: 10.1186/s12958-020-00679-9
PMID: 33308222	Sample: Cell Culture Supernatant

TOXICOLOGY AND APPLIED PHARMACOLOGY (2020) IF: 3.347

2,5-Hexanedione influences primordial follicular development in cultured neonatal mouse ovaries by interfering with the PI3K signaling pathway via miR-214-3p

DOI: 10.1016/j.taap.2020.115335
PMID: 33197454	Sample: Cell Culture Supernatant

JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2020) IF: 3.606

Protective roles and mechanisms of rosmarinic acid in cyclophosphamide‐induced premature ovarian failure

DOI: 10.1002/jbt.22591
	Sample: Serum

JOURNAL OF REPRODUCTIVE IMMUNOLOGY (2020) IF: 4.018

Ovalbumin causes impairment of preimplantation embryonic growth in asthma-induced mice

DOI: 10.1016/j.jri.2020.103240
PMID: 33166807	Sample: Serum

BIOLOGY OF REPRODUCTION (2022) IF: 4.285

Pre-implantation triclosan exposure alters uterine receptivity through affecting tight junction protein.

DOI: 10.1093/biolre/ioac092
	Sample: Serum

BIOLOGY OF REPRODUCTION (2021) IF: 4.285

Chronic overload of concentration-dependent iron exerts different effects on ovarian function in C57BL/6J mice

DOI: 10.1093/biolre/ioab040
	Sample: Serum

JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36

Network pharmacology-based study on immunomodulatory mechanism of danggui-yimucao herb pair for the treatment of RU486-induced abortion

DOI: 10.1016/j.jep.2021.114609
PMID: 34508802	Sample: Serum

Human Cell (2022) IF: 4.374

Repeated controlled ovarian stimulation-induced ovarian and uterine damage in mice through the PI3K/AKT signaling pathway

DOI: 10.1007/s13577-022-00829-8
	Sample: Serum

Metallomics (2022) IF: 4.636

Transcriptomic analysis reveals the effects of maternal selenium deficiency on placental transport, hormone synthesis, and immune response in mice

DOI: 10.1093/mtomcs/mfac062
PMID: 36002020	Sample: Serum,Placenta

ENVIRONMENTAL POLLUTION (2019) IF: 5.714

Exposure to DEHP or its metabolite MEHP promotes progesterone secretion and inhibits proliferation in mouse placenta or JEG-3 cells

DOI: 10.1016/j.envpol.2019.113593
	Sample: Cell Culture Medium

International Journal of Nanomedicine (2022) IF: 7.033

Biocompatible Assessment of Erythrocyte Membrane-Camouflaged Polymeric PLGA Nanoparticles in Pregnant Mice: Both on Maternal and Fetal/Juvenile Mice

DOI: 10.2147/IJN.S384906
	Sample: Serum

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2022) IF: 7.129

Maternal exposure to cadmium from puberty through lactation induces abnormal reproductive development in female offspring

DOI: 10.1016/j.ecoenv.2022.113927
PMID: 35908533	Sample: Serum

ENVIRONMENT INTERNATIONAL (2020) IF: 7.577

Environmental cadmium exposure induces fetal growth restriction via triggering PERK-regulated mitophagy in placental trophoblasts

DOI: 10.1016/j.envint.2020.106319
PMID: 33348103	Sample: Serum,Amniotic Fluid,Cell Medium,Cell Lysate

ENVIRONMENT INTERNATIONAL (2020) IF: 7.577

Bisphenol A promotes autophagy in ovarian granulosa cells by inducing AMPK/mTOR/ULK1 signalling pathway

DOI: 10.1016/j.envint.2020.106298
PMID: 33387880	Sample: Cell Culture Supernatant,Serum

ENVIRONMENTAL POLLUTION (2022) IF: 9.988

Betulinic acid alleviates zearalenone-induced uterine injury in mice

DOI: 10.1016/j.envpol.2022.120435
PMID: 36257561	Sample: Serum

JOURNAL OF HAZARDOUS MATERIALS (2021) IF: 10.588

Di (2-ethyl-hexyl) phthalate disrupts placental growth in a dual blocking mode

DOI: 10.1016/j.jhazmat.2021.126815
	Sample: Serum

Redox Biology (2021) IF: 11.799

Melatonin protects against environmental stress-induced fetal growth restriction via suppressing ROS-mediated GCN2/ATF4/BNIP3-dependent mitophagy in placental trophoblasts

DOI: 10.1016/j.redox.2021.101854
PMID: 33454563	Sample: Placentae Tissue

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Cat. No	E-OSEL-M0006
Species

QuicKey Pro Mouse Pg(Progesterone) ELISA Kit

To Purchase E-OSEL-M0006

QuicKey Pro Series

Product Details

Properties

Test Principle

Kit components & Storage

Technical Data

Precision

Recovery

Linearity

Target Information

Assay Procedures

Citations

Reproductive Biology and Endocrinology (2021) IF: 5.211

Journal of Ayurveda and Integrative Medicine (2020)

GENE (2018) IF: 2.498

Evidence-based Complementary and Alternative Medicine (2021) IF: 2.63

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL (2022) IF: 2.723

Reproductive Biology and Endocrinology (2020) IF: 3.235

TOXICOLOGY AND APPLIED PHARMACOLOGY (2020) IF: 3.347

JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2020) IF: 3.606

JOURNAL OF REPRODUCTIVE IMMUNOLOGY (2020) IF: 4.018

BIOLOGY OF REPRODUCTION (2022) IF: 4.285

BIOLOGY OF REPRODUCTION (2021) IF: 4.285

JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36

Human Cell (2022) IF: 4.374

Metallomics (2022) IF: 4.636

ENVIRONMENTAL POLLUTION (2019) IF: 5.714

International Journal of Nanomedicine (2022) IF: 7.033

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2022) IF: 7.129

ENVIRONMENT INTERNATIONAL (2020) IF: 7.577

ENVIRONMENT INTERNATIONAL (2020) IF: 7.577

ENVIRONMENTAL POLLUTION (2022) IF: 9.988

JOURNAL OF HAZARDOUS MATERIALS (2021) IF: 10.588

Redox Biology (2021) IF: 11.799

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