Product Summary

Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Sensitivity	0.14 ng/mL
Detection Range	0.31-20 ng/mL
Sample Volume	50 μL
Total Assay Time	1 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse T in samples.No significant cross-reactivity or interference between Mouse T and analogues was observed
Recovery	80%-120%
Sample Type	serum, plasma
Detection Method	Colorimetric method, ELISA, Competitive
Assay Type	Competitive-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	6 months

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse T. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse T are added to the micro ELISA plate wells. Mouse T in samples (or standards) competes with a fixed amount of T on the solid phase supporter for sites on the HRP linked detection antibody specific to T. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Mouse T in the samples is then determined by comparing the OD of the samples to the standard curve.

Background

Testosterone is the principal androgen, or male sex hormone. One of the group of compounds known as anabolic steroids, testosterone is secreted by the testes but is also synthesized in small quantities in the ovaries, cortices of the adrenal glands, and placenta, usually from cholesterol. Testosterone is necessary in the fetus for the development of male external genitalia. Testosterone also stimulates protein synthesis and accounts for the greater muscular development of the male.

Research Area

Signal Transduction , Signal Transduction , Epigenetics And Nuclear Signaling , Developmental Biology