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QuicKey Pro Mouse T(Testosterone)ELISA Kit

Citations(45)

  • Cat.No.:E-OSEL-M0003

  • Reactivity: Mouse

To Purchase E-OSEL-M0003

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $520
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QuicKey Pro Series

Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Product Details

Properties

Assay type Competitive-ELISA
Format 96T/48T/24
Assay time 1.5h
Detection range 0.31-20 ng/mL
Sensitivity 0.14 ng/mL
Sample type &Sample volume serum and plasma; 50μL
Specificity This kit recognizes T in samples. No significant cross-reactivity or interference between T and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of T concentrations in serum and plasma.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse T. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse T are added to the micro ELISA plate wells. Mouse T in samples (or standards) competes with a fixed amount of T on the solid phase supporter for sites on the HRP linked detection antibody specific to T. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Mouse T in the samples is then determined by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the table.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
24T: 8 wells ×3 strips
96T*5: 5 plates, 96T 		2-8℃, 1 months
Reference Standard 96T: 2 vials
48T/24T: 1 vial
96T*5: 10 vials 		2-8℃, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100×) 96T: 1 vial, 60 μL
48T/24T: 1 vial, 30 μL
96T*5: 5 vials, 60 μL 		2-8℃(Protect from light)
Reference Standard & Sample Diluent 96T/48T/24T: 1 vial, 20 mL
96T*5: 5 vials, 20 mL		 2-8℃
HRP Linked Ab Diluent 96T/48T/24T: 1 vial, 14 mL
96T*5: 5 vials, 14 mL
Concentrated Wash Buffer(25×) 96T/48T/24T: 1 vial, 30 mL
96T*5: 5 vials, 30 mL
Substrate Reagent 96T/48T/24T: 1 vial, 10 mL
96T*5: 5 vials, 10 mL		 2-8℃(Protect from light)
Stop Solution 96T/48T/24T: 1 vial, 10 mL
96T*5: 5 vials, 10 mL		 2-8℃
Plate Sealer 96T/48T/24T: 5 pieces
96T*5: 25 pieces
Product Description 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level T were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level T were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 1.02 1.87 8.67 1.00 2.01 7.85
Standard deviation 0.06 0.11 0.38 0.06 0.10 0.43
CV (%) 5.89 5.63 4.44 5.80 5.16 5.49

Recovery

The recovery of T spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-106 99
EDTA plasma(n=8) 84-96 91

Linearity

Samples were spiked with high concentrations of Mouse T and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Assay Procedures

Citations

  1. JOURNAL OF HAZARDOUS MATERIALS (2020) IF: 10.588
    Polystyrene microplastics induced male reproductive toxicity in mice

    DOI: 10.1016/j.jhazmat.2020.123430

    PMID: 32659591

    		 Sample: Serum
  2. JOURNAL OF HAZARDOUS MATERIALS (2021) IF: 10.588
    Di (2-ethyl-hexyl) phthalate disrupts placental growth in a dual blocking mode

    DOI: 10.1016/j.jhazmat.2021.126815
    Sample: serum
  3. JOURNAL OF HAZARDOUS MATERIALS (2020) IF: 9.038
    Multiple transcriptomic profiling: p53 signaling pathway is involved in DEHP-induced prepubertal testicular injury via promoting cell apoptosis and inhibiting cell proliferation of Leydig cells

    DOI: 10.1016/j.jhazmat.2020.124316

    PMID: 33162236

    		 Sample: Serum
  4. Particle and Fibre Toxicology (2022) IF: 9.4
    Chronic exposure to polystyrene microplastics induced male reproductive toxicity and decreased testosterone levels via the LH-mediated LHR/cAMP/PKA/StAR pathway

    DOI: 10.1186/s12989-022-00453-2

    PMID: 35177090

    		 Sample: serum
  5. CHEMOSPHERE (2020) IF: 7.086
    Interleukin 17A deficiency alleviates fluoride-induced testicular injury by inhibiting the immune response and apoptosis

    DOI: 10.1016/j.chemosphere.2020.128178
    Sample: serum
  6. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2023) IF: 7.129
    p38 mediates T-2 toxin-induced Leydig cell testosterone synthesis disorder

    DOI: 10.1016/j.ecoenv.2023.114695
    Sample: testis,TM3 cell
  7. Frontiers in Endocrinology (2022) IF: 6.055
    Role of EGFR expressed on the granulosa cells in the pathogenesis of polycystic ovarian syndrome.

    DOI: 10.3389/fendo.2022.971564
    Sample: serum
  8. International Journal of Nanomedicine (2022) IF: 6.4
    Pseudoephedrine Nanoparticles Alleviate Adriamycin-Induced Reproductive Toxicity Through the GnRhR Signaling Pathway

    DOI: 10.2147/IJN.S348673

    PMID: 35401001

    		 Sample: serum
  9. BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 6.53
    Ephedra herb reduces adriamycin-induced testicular toxicity by upregulating the gonadotropin-releasing hormone signalling pathway

    DOI: 10.1016/j.biopha.2022.113061

    PMID: 35658231

    		 Sample: serum
  10. SCIENCE OF THE TOTAL ENVIRONMENT (2020) IF: 6.551
    Exposure to hyperandrogen drives ovarian dysfunction and fibrosis by activating the NLRP3 inflammasome in mice

    DOI: 10.1016/j.scitotenv.2020.141049
    Sample: serum
  11. ENVIRONMENTAL POLLUTION (2020) IF: 6.793
    Increased m6A modification of RNA methylation related to the inhibition of demethylase FTO contributes to MEHP-induced Leydig cell injury☆

    DOI: 10.1016/j.envpol.2020.115627
    Sample: Cell culture supernatant
  12. Food Science and Human Wellness (2022) IF: 5.154
    The protective effects of Procyanidin C-1 on bisphenol a-induced testicular dysfunction in aged mice

    DOI: 10.1016/j.fshw.2022.03.020
    Sample: serum
  13. Reproductive Biology and Endocrinology (2021) IF: 5.211
    Irisin deletion induces a decrease in growth and fertility in mice

    DOI: 10.1186/s12958-021-00702-7

    PMID: 33581723

    		 Sample: Serum
  14. Reproductive Biology and Endocrinology (2022) IF: 5.211
    Betaine attenuate chronic restraint stress-induced changes in testicular damage and oxidative stress in male mice

    DOI: 10.1186/s12958-022-00949-8

    PMID: 35597951

    		 Sample: serum
  15. CLINICAL SCIENCE (2018) IF: 5.22
    CTRP3 attenuates high-fat diet-induced male reproductive dysfunction in mice

    DOI: 10.1042/CS20180179
    Sample: Serum
  16. Scientific Reports (2016) IF: 5.228
    Knockout of BRD7 results in impaired spermatogenesis and male infertility

    DOI: 10.1038/srep21776
    Sample: serum
  17. FOOD AND CHEMICAL TOXICOLOGY (2023) IF: 5.572
    Polystyrene nanoplastics inhibit StAR expression by activating HIF-1α via ERK1/2 MAPK and AKT pathways in TM3 Leydig cells and testicular tissues of mice

    DOI: 10.1016/j.fct.2023.113634
    Sample: testis,serum
  18. FOOD AND CHEMICAL TOXICOLOGY (2022) IF: 5.572
    Exposure to acrylamide inhibits testosterone production in mice testes and Leydig cells by activating ERK1/2 phosphorylation

    DOI: 10.1016/j.fct.2022.113576
    Sample: serum,TM3 Leydig cells
  19. CHEMOSPHERE (2020) IF: 5.778
    Effects of fluoride on PIWI-interacting RNA expression profiling in testis of mice

    DOI: 10.1016/j.chemosphere.2020.128727

    PMID: 33213873

    		 Sample: Serum
  20. Scientific Reports (2019) IF: 4.011
    Gestational exposure to an epidemiologically defined mixture of phthalates leads to gonadal dysfunction in mouse offspring of both sexes

    DOI: 10.1038/s41598-019-42377-6
    Sample: plasma
  21. Genes (2021) IF: 4.096
    DYRK1A Overexpression in Mice Downregulates the Gonadotropic Axis and Disturbs Early Stages of Spermatogenesis

    DOI: 10.3390/genes12111800

    PMID: 34828406

    		 Sample: plasma
  22. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2019) IF: 4.183
    Sulforaphane Protect Against Cadmium-Induced Oxidative Damage in mouse Leydigs Cells by Activating Nrf2/ARE Signaling Pathway

    DOI: 10.3390/ijms20030630

    PMID: 30717178

    		 Sample: Tissue homogenate
  23. TOXICOLOGY (2021) IF: 4.221
    Environmentally relevant perinatal exposure to DBP disturbs testicular development and puberty onset in male mice

    DOI: 10.1016/j.tox.2021.152860

    PMID: 34280466

    		 Sample: Serum
  24. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2021) IF: 4.223
    Multiple transcriptomic profiling: potential novel metabolism-related genes predict prepubertal testis damage caused by DEHP exposure

    DOI: 10.1007/s11356-021-16701-5

    PMID: 34595713

    		 Sample: serum
  25. Scientific Reports (2017) IF: 4.259
    Diet-induced obesity impairs spermatogenesis: a potential role for autophagy

    DOI: 10.1038/srep43475
    Sample: Serum
  26. BIOLOGY OF REPRODUCTION (2022) IF: 4.285
    Loss of raptor induces Sertoli cells into an undifferentiated state in mice

    DOI: 10.1093/biolre/ioac104

    PMID: 35594452

    		 Sample: gonads
  27. International Journal of Nanomedicine (2019) IF: 4.471
    CeO2NPs relieve radiofrequency radiation, improve testosterone synthesis, and clock gene expression in Leydig cells by enhancing antioxidation

    DOI: 10.2147/IJN.S206561
    Sample: Leydig cells
  28. BIOORGANIC CHEMISTRY (2020) IF: 4.831
    Two resveratrol analogs, pinosylvin and 4,4′-dihydroxystilbene, improve oligoasthenospermia in a mouse model by attenuating oxidative stress via the Nrf2-ARE pathway

    DOI: 10.1016/j.bioorg.2020.104295
    Sample: serum
  29. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2020) IF: 4.872
    LncRNAs activate longevity regulation pathway due to aging of Leydig cells caused by DEHP exposure: A transcriptome-based study

    DOI: 10.1016/j.ecoenv.2020.111798

    PMID: 33360214

    		 Sample: Cell culture supernatant
  30. Scientific Reports (2022) IF: 4.996
    Effects of Cordyceps militaris fermentation products on reproductive development in juvenile male mice

    DOI: 10.1038/s41598-022-18066-2

    PMID: 35962055

    		 Sample: serum
  31. REPRODUCTIVE TOXICOLOGY (2021) IF: 3.143
    Weighted gene coexpression network analysis reveals ESR1, FLNA and Furin as hub genes for DEHP-induced prepubertal testicular injury

    DOI: 10.1016/j.reprotox.2021.10.011

    PMID: 34718101

    		 Sample: serum
  32. JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY (2020) IF: 3.245
    Possible differences in the mechanism of malignant transformation of HaCaT cells by arsenite and its dimethyl metabolites, particularly dimethylthioarsenics

    DOI: 10.1016/j.jtemb.2020.126544
    Sample: Serum
  33. Toxins (2018) IF: 3.273
    The Protective Role of Bacillus velezensis A2 on the Biochemical and Hepatic Toxicity of Zearalenone in Mice

    DOI: 10.3390/toxins10110449

    PMID: 30384460

    		 Sample: Serum
  34. BIOLOGY OF REPRODUCTION (2020) IF: 3.322
    Loss of Fbxw7 in Sertoli cells impairs testis development and causes infertility in mice

    DOI: 10.1093/biolre/ioz230
    Sample: plasma
  35. TOXICOLOGY LETTERS (2014) IF: 3.355
    Differential proteome and gene expression reveal response to carbon ion irradiation in pubertal mice testes

    DOI: 10.1016/j.toxlet.2014.01.001
    Sample: Serum
  36. Toxicology Research (2021) IF: 3.524
    The modulatory impacts of Glycyrrhiza glabra extract against methotrexate-induced testicular dysfunction and oxidative stress

    DOI: 10.1093/toxres/tfab049
    Sample: serum
  37. TOXICOLOGY LETTERS (2020) IF: 3.569
    Downregulation of vdac2 inhibits spermatogenesis via JNK and P53 signalling in mice exposed to cadmium

    DOI: 10.1016/j.toxlet.2020.03.011

    PMID: 32199951

    		 Sample: Cell culture supernatant
  38. Endocrinology and Metabolism (2022) IF: 3.607
    Fancd2os Reduces Testosterone Production by Inhibiting Steroidogenic Enzymes and Promoting Cellular Apoptosis in Murine Testicular Leydig Cells

    DOI: 10.3803/EnM.2022.1431
    Sample: serum,TM3 cell
  39. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2018) IF: 3.974
    Sulphur dioxide and arsenic affect male reproduction via interfering with spermatogenesis in mice

    DOI: 10.1016/j.ecoenv.2018.08.109

    PMID: 30195209

    		 Sample: Serum
  40. International Journal of Environmental Research and Public Health (2017) IF: 2.101
    1950MHz Radio Frequency Electromagnetic Radiation Inhibits Testosterone Secretion of Mouse Leydig Cells

    DOI: 10.3390/ijerph15010017
    Sample: TM3 cell
  41. BIOLOGICAL TRACE ELEMENT RESEARCH (2019) IF: 2.431
    The Footprints of Oxidative Stress and Mitochondrial Impairment in Arsenic Trioxide-Induced Testosterone Release Suppression in Pubertal and Mature F1-Male Balb/c Mice via the Downregulation of 3β-HSD, 17β-HSD, and CYP11a Expression

    DOI: 10.1007/s12011-019-01815-2
    Sample: Serum,Tissue homogenate
  42. Reproductive Sciences (2020) IF: 2.616
    Ascorbic Acid and Alpha-Tocopherol Contribute to the Therapy of Polycystic Ovarian Syndrome in Mouse Models

    DOI: 10.1007/s43032-020-00273-9

    PMID: 32725591

    		 Sample: Serum
  43. Evidence-based Complementary and Alternative Medicine (2021) IF: 2.63
    Effect of Electroacupuncture on Reproductive Disorders and Insulin Resistance in a Murine Polycystic Ovary Syndrome Model

    DOI: 10.1155/2021/9968463

    PMID: 34987599

    		 Sample: serum
  44. BIOLOGY OF REPRODUCTION (2019) IF: 2.96
    Sulforaphane ameliorates high-fat diet-induced spermatogenic deficiency in mice

    DOI: 10.1093/biolre/ioz067
    Sample: serum
  45. TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH (2021) IF: 0.533
    Effect of total flavonoids of Cuscuta chinensis Lam. (Convolvulaceae) on oxidative stress injury in mouse testis and epididymis, and on serum levels of reproductive hormones in oligoasthenospermia mice model

    DOI: 10.4314/tjpr.v18i6.15
    Sample: Serum,Tissue homogenate
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