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Cat.No.:E-OSEL-R0006
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Assay type | Sandwich-ELISA |
Format | 96T/48T/24 |
Assay time | 1.5h |
Detection range | 31.25-1000 ng/mL |
Sensitivity | 6.43 ng/mL |
Sample type &Sample volume | serum and plasma; 50μL |
Specificity | This kit recognizes ADP/Acrp30 in samples. No significant cross-reactivity or interference between ADP/Acrp30 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of ADP/Acrp30 concentrations in serum and plasma. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 24T: 8 wells ×3 strips 96T*5: 5 plates, 96T |
2-8℃, 1 months |
Reference Standard | 96T: 2 vials
48T/24T: 1 vial 96T*5: 10 vials |
2-8℃, use the reconstituted standard within 24h |
Concentrated HRP Linked Detection Ab(100×) | 96T: 1 vial, 60 μL
48T/24T: 1 vial, 30 μL 96T*5: 5 vials, 60 μL |
2-8℃(Protect from light) |
Reference Standard & Sample Diluent | 96T/48T/24T: 1 vial, 20 mL 96T*5: 5 vials, 20 mL |
2-8℃ |
HRP Linked Ab Diluent | 96T/48T/24T: 1 vial, 14 mL 96T*5: 5 vials, 14 mL |
|
Concentrated Wash Buffer(25×) | 96T/48T/24T: 1 vial, 30 mL 96T*5: 5 vials, 30 mL |
|
Substrate Reagent | 96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL |
2-8℃(Protect from light) |
Stop Solution | 96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL |
2-8℃ |
Plate Sealer | 96T/48T/24T: 5 pieces 96T*5: 25 pieces |
|
Product Description | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level ADP/Acrp30 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level ADP/Acrp30 were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (ng/mL) | 52.94 | 153.56 | 371.41 | 50.14 | 155.02 | 346.08 |
Standard deviation | 2.94 | 8.06 | 11.14 | 2.58 | 8.79 | 17.65 |
CV (%) | 5.55 | 5.25 | 3.00 | 5.15 | 5.67 | 5.10 |
The recovery of ADP/Acrp30 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 87-100 | 95 |
EDTA plasma(n=8) | 95-111 | 101 |
Samples were spiked with high concentrations of Rat ADP/Acrp30 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Synonyms | Adiponectin,GBP28, ACDC, APM1, ADPN, AdipoQ, ADIPQTL1 |
Research Area | Cancer, Cardiovascular, Metabolism, Neuroscience, Signal transduction, Stem cells |
PMID: 33142857 |
Sample: Plasma |
PMID: 34943134 |
Sample: serum |
PMID: 33562069 |
Sample: Serum |
PMID: 36297569 |
Sample: serum |
Sample: Serum |
PMID: 36150640 |
Sample: plasma |
Sample: serum |
Sample: serum |
DOI: 10.3390/ijms22105400 |
|
PMID: 34065591 |
Sample: Tissue homogenate |
PMID: 34229480 |
Sample: serum,testis |
Sample: serum,adipose tissues |
PMID: 35835020 |
Sample: Serum |
Sample: Serum |
Sample: plasma |
Sample: Serum |
DOI: 10.7717/peerj.13990 |
|
PMID: 36213511 |
Sample: serum |
PMID: 33002592 |
Sample: Serum |
PMID: 33359182 |
Sample: Plasma |
Sample: serum |
DOI: 10.1111/jfbc.14442 |
|
PMID: 36165438 |
Sample: serum |
DOI: 10.1002/ptr.6369 |
|
Sample: blood |
Sample: serum |
PMID: 32424476 |
Sample: Serum |
Sample: Plasma |
Sample: Serum |
Sample: Serum |
Sample: plasma |
Sample: Serum |
DOI: 10.1111/andr.12824 |
|
Sample: Tissue homogenate |
Sample: Cell culture supernatant |
DOI: 10.1155/2015/602313 |
|
Sample: Serum |
Sample: serum |
Sample: Placenta |
Sample: plasma |
DOI: 10.3892/br.2017.884 |
|
Sample: serum |
Sample: Serum |