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Current Position:Home>>Hot>>QuicKey Pro ELISA>> QuicKey Pro Rat CORT(Corticosterone) ELISA Kit

QuicKey Pro Rat CORT(Corticosterone) ELISA Kit

Citations(49)

  • Cat.No.:E-OSEL-R0002

  • Reactivity: Rat

To Purchase E-OSEL-R0002

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
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QuicKey Pro Series

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Product Details

Properties

Assay type Competitive-ELISA
Format 96T/48T/24
Assay time 1.5h
Detection range 4.69-300 ng/mL
Sensitivity 1.87 ng/mL
Sample type &Sample volume serum, plasma, urine, saliva; 50μL
Specificity This kit recognizes Cort in samples. No significant cross-reactivity or interference between Cort and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Cort concentrations in serum, plasma, urine, saliva.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat Cort. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat Cort are added to the micro ELISA plate wells. Rat Cort in samples (or standards) competes with a fixed amount of Cort on the solid phase supporter for sites on the HRP linked detection antibody specific to Cort. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Rat Cort in the samples is then determined by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the table.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
24T: 8 wells ×3 strips
96T*5: 5 plates, 96T 		2-8℃, 1 months
Reference Standard 96T: 2 vials
48T/24T: 1 vial
96T*5: 10 vials 		2-8℃, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100×) 96T: 1 vial, 60 μL
48T/24T: 1 vial, 30 μL
96T*5: 5 vials, 60 μL 		2-8℃(Protect from light)
Reference Standard & Sample Diluent 96T/48T/24T: 1 vial, 20 mL
96T*5: 5 vials, 20 mL		 2-8℃
HRP Linked Ab Diluent 96T/48T/24T: 1 vial, 14 mL
96T*5: 5 vials, 14 mL
Concentrated Wash Buffer(25×) 96T/48T/24T: 1 vial, 30 mL
96T*5: 5 vials, 30 mL
Substrate Reagent 96T/48T/24T: 1 vial, 10 mL
96T*5: 5 vials, 10 mL		 2-8℃(Protect from light)
Stop Solution 96T/48T/24T: 1 vial, 10 mL
96T*5: 5 vials, 10 mL		 2-8℃
Plate Sealer 96T/48T/24T: 5 pieces
96T*5: 25 pieces
Product Description 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Cort were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Cort were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 13.64 37.16 121.75 14.17 37.85 113.64
Standard deviation 0.70 2.02 4.49 0.77 2.27 4.78
CV (%) 5.12 5.43 3.69 5.41 6.00 4.21

Recovery

The recovery of Cort spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-105 97
EDTA plasma(n=8) 91-105 98

Linearity

Samples were spiked with high concentrations of Rat Cort and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsCorticosterone，CORT
Research AreaCell biology

Assay Procedures

Citations

  1. Cell Reports (2022) IF: 9.423
    Gut microbiota shapes social dominance through modulating HDAC2 in the medial prefrontal cortex

    DOI: 10.1016/j.celrep.2022.110478
    Sample: plasma
  2. ENVIRONMENTAL POLLUTION (2022) IF: 9.988
    Acute exposure to microcystins affects hypothalamic-pituitary axes of male rats

    DOI: 10.1016/j.envpol.2022.120843
    Sample: serum
  3. SCIENCE OF THE TOTAL ENVIRONMENT (2021) IF: 7.963
    Effects of acute exposure to microcystins on hypothalamic-pituitary-adrenal (HPA), -gonad (HPG) and -thyroid (HPT) axes of female rats

    DOI: 10.1016/j.scitotenv.2021.145196

    PMID: 34030373

    		 Sample: Serum
  4. MOLECULAR NEUROBIOLOGY (2016) IF: 5.397
    Nr3C1-Bhlhb2 Axis Dysregulation Is Involved in the Development of Attention Deficit Hyperactivity

    DOI: 10.1007/s12035-015-9679-z
    Sample: Plasma
  5. Aging-US (2019) IF: 5.515
    GSK-3β and ERK1/2 incongruously act in tau hyperphosphorylation in SPS-induced PTSD rats

    DOI: 10.18632/aging.102303

    PMID: 31548435

    		 Sample: Tissue homogenate
  6. Frontiers in Pharmacology (2022) IF: 5.988
    Andrographolide exerted anti-inflammatory effects thereby reducing sex hormone synthesis in LPS-induced female rats, but had no effect on hormone production in healthy ones.

    DOI: 10.3389/fphar.2022.980064
    Sample: serum
  7. INFLAMMATION (2021) IF: 4.092
    Neuropeptide W Attenuates Oxidative Multi-Organ Injury in Rats Induced with Intra-Abdominal Sepsis

    DOI: 10.1007/s10753-021-01545-5
    Sample: serum
  8. Frontiers in Psychiatry (2021) IF: 4.157
    Post-weaning Social Isolated Flinders Sensitive Line Rats Display Bio-Behavioural Manifestations Resistant to Fluoxetine: A Model of Treatment-Resistant Depression

    DOI: 10.3389/fpsyt.2021.688150

    PMID: 34867504

    		 Sample: plasma
  9. Food Bioscience (2021) IF: 4.24
    Evaluation of in-vivo model for vitamin A bioavailability from vitamin A loaded caseinate complex

    DOI: 10.1016/j.fbio.2021.101174
    Sample: serum
  10. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 4.36
    Antidepressant, anti-amnesic and vasoprotective effect of Bombax costatum Pellegr. & Vuillet aqueous stem bark extract on chronic mild unpredictable stress induced in rat

    DOI: 10.1016/j.jep.2022.115315

    PMID: 35487448

    		 Sample: plasma
  11. BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
    Sodium butyrate arrests pancreato-hepatic synchronous uric acid and lipid dysmetabolism in high fat diet fed Wistar rats

    DOI: 10.1016/j.biopha.2020.110994

    PMID: 33197764

    		 Sample: Plasma
  12. ENDOCRINOLOGY (2022) IF: 4.736
    Scream sound induced chronic psychological stress results in diminished ovarian reserve in adult female rat

    DOI: 10.1210/endocr/bqac042
    Sample: serum
  13. NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2020) IF: 3
    Impact of some oral hypoglycemic agents on type 2 diabetes-associated depression and reserpine-induced depression in rats: the role of brain oxidative stress and inflammation

    DOI: 10.1007/s00210-020-01838-w

    PMID: 32077986

    		 Sample: Serum
  14. BEHAVIOURAL BRAIN RESEARCH (2017) IF: 3.002
    Injection of oxytocin into paraventricular nucleus reverses depressive-like behaviors in the postpartum depression rat model

    DOI: 10.1016/j.bbr.2017.09.012
    Sample: Plasma
  15. Pharmacological Reports (2021) IF: 3.027
    Development and treatment of cognitive inflexibility in sub-chronic stress–re-stress (SRS) model of PTSD

    DOI: 10.1007/s43440-020-00198-9
    Sample: Plasma
  16. BEHAVIOURAL BRAIN RESEARCH (2018) IF: 3.173
    The effects of acute foot shock stress on empathy levels in rats

    DOI: 10.1016/j.bbr.2018.04.043

    PMID: 29709611

    		 Sample: Serum,Tissue homogenate
  17. LIFE SCIENCES (2018) IF: 3.234
    Long term treadmill exercise performed to chronic social isolated rats regulate anxiety behavior without improving learning

    DOI: 10.1016/j.lfs.2018.03.029
    Sample: Plasma
  18. American Journal of Translational Research (2019) IF: 3.266
    Therapeutic effect of low-intensity pulsed ultrasound on temporomandibular joint injury induced by chronic sleep deprivation in rats

    PMID: 31312347

    		 Sample: serum
  19. ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY (2020) IF: 3.292
    Insulin resistance and depressed cardiac G6PD activity induced by glucocorticoid exposure during pregnancy are attenuated by maternal estrogen-progestin therapy

    DOI: 10.1016/j.etap.2020.103423

    PMID: 32492534

    		 Sample: Plasma
  20. BEHAVIOURAL BRAIN RESEARCH (2022) IF: 3.352
    Postpartum depression in rats causes poor maternal care and neurochemical alterations on dams and long-lasting impairment in sociability on the offspring

    DOI: 10.1016/j.bbr.2022.114082

    PMID: 36041571

    		 Sample: serum
  21. BIOMEDICINE & PHARMACOTHERAPY (2018) IF: 3.457
    Akebia saponin D reverses corticosterone hypersecretion in an Alzheimer’s disease rat model

    DOI: 10.1016/j.biopha.2018.07.149
    Sample: Tissue homogenate
  22. JOURNAL OF ETHNOPHARMACOLOGY (2020) IF: 3.69
    Tandem mass tag-based proteomic analysis reveals the treatment mechanism of Bushen Huoxue Formula on psychological stress-induced premature ovarian insufficiency

    DOI: 10.1016/j.jep.2020.112870

    PMID: 32311483

    		 Sample: Serum
  23. Heliyon (2019) IF: 3.776
    Adverse effects of noise stress on glucose homeostasis and insulin resistance in Sprague-Dawley rats

    DOI: 10.1016/j.heliyon.2019.e03004

    PMID: 31890958

    		 Sample: Serum
  24. NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2019) IF: 2.058
    Sodium butyrate recovers high-fat diet-fed female Wistar rats from glucose dysmetabolism and uric acid-associated cardiac tissue damage

    DOI: 10.1007/s00210-019-01679-2
    Sample: plasma
  25. NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2019) IF: 2.058
    Involvement of glucocorticoid and mineralocorticoid receptors in lipid accumulation and depressed G6PD activity in the livers of rats treated with postpartum oral estrogen-progestin

    DOI: 10.1007/s00210-019-01634-1

    PMID: 30903201

    		 Sample: Plasma
  26. STRESS-THE INTERNATIONAL JOURNAL ON THE BIOLOGY OF STRESS (2019) IF: 2.168
    Parental pre-conception stress status and risk for anxiety in rat offspring: specific and sex-dependent maternal and paternal effects

    DOI: 10.1080/10253890.2019.1619075
    Sample: plasma
  27. CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2018) IF: 2.21
    Oral hormonal therapy with ethinylestradiol–levonorgestrel improves insulin resistance, obesity, and glycogen synthase kinase-3 independent of circulating mineralocorticoid in estrogen-deficient rats

    DOI: 10.1139/cjpp-2017-0630
    Sample: plasma
  28. CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2018) IF: 2.21
    Ameliorative effect of low-dose spironolactone on obesity and insulin resistance is through replenishment of estrogen in ovariectomized rats

    DOI: 10.1139/cjpp-2018-0416

    PMID: 30422674

    		 Sample: Plasma
  29. Pharmacological Reports (2016) IF: 2.251
    Anti-inflammatory and antithrombotic effects of nicotine exposure in oral contraceptive-induced insulin resistance are glucocorticoid-independent

    DOI: 10.1016/j.pharep.2016.12.010
    Sample: plasma
  30. INTERNATIONAL JOURNAL OF NEUROSCIENCE (2022) IF: 2.292
    Alteration of the α5 GABA receptor and 5HTT lead to cognitive deficits associated with major depressive-like behaviors in a 14-day combined stress rat model

    DOI: 10.1080/00207454.2021.2019033

    PMID: 34937496

    		 Sample: plasma
  31. Experimental and Therapeutic Medicine (2018) IF: 2.447
    Analysis of gene expression and functional changes of adrenal gland in a rat model of kidney yang deficiency syndrome treated with Sini decoction

    DOI: 10.3892/etm.2018.6521

    PMID: 30214533

    		 Sample: Serum
  32. INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE (2022) IF: 2.457
    Maternal prenatal stress and depression-like behavior associated with hippocampal and cortical neuroinflammation in the offspring: An experimental study

    DOI: 10.1002/jdn.10176

    PMID: 35261075

    		 Sample: plasma
  33. INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE (2022) IF: 2.457
    Environmental enrichment as a strategy: attenuates the anxiety and memory impairment in social isolation stress

    DOI: 10.1002/jdn.10205

    PMID: 35724417

    		 Sample: plasma
  34. PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR (2018) IF: 2.538
    Acetaminophen (paracetamol) affects empathy-like behavior in rats: Dose-response relationship

    DOI: 10.1016/j.pbb.2018.10.004

    PMID: 30359628

    		 Sample: Serum,Tissue homogenate
  35. BEHAVIOURAL BRAIN RESEARCH (2019) IF: 2.77
    Effects of corticosterone on the expression of mature brain-derived neurotrophic factor (mBDNF) and proBDNF in the hippocampal dentate gyrus

    DOI: 10.1016/j.bbr.2019.03.010

    PMID: 30851317

    		 Sample: Plasma
  36. BRAIN RESEARCH (2019) IF: 2.929
    Effect of enriched environment and predictable chronic stress on spatial memory in adolescent rats: Predominant expression of BDNF, nNOS, and interestingly malondialdehyde in the right hippocampus

    DOI: 10.1016/j.brainres.2019.146326
    Sample: serum
  37. PHARMACEUTICAL BIOLOGY (2020) IF: 2.971
    Lilium davidii extract alleviates p‑chlorophenylalanine‑induced insomnia in rats through modification of the hypothalamic-related neurotransmitters, melatonin and homeostasis of the hypothalamic-pituitary-adrenal axis

    DOI: 10.1080/13880209.2020.1812674

    PMID: 32924742

    		 Sample: serum
  38. ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY (2017) IF: 1.22
    Ameliorative effect of nicotine exposure on insulin resistance is accompanied by decreased cardiac glycogen synthase kinase-3 and plasminogen activator inhibitor-1 during oral oestrogen-progestin therapy

    DOI: 10.1080/13813455.2017.1369549
    Sample: Plasma,Tissue homogenate
  39. ACTA NEUROBIOLOGIAE EXPERIMENTALIS (2022) IF: 1.269
    Repeated acetaminophen administration damaged hippocampal tissue but did not affect prefrontal cortex or anxiety behaviors.

    DOI: 10.55782/ane-2022-015
    Sample: serum
  40. Experimental and Therapeutic Medicine (2016) IF: 1.28
    Schisandra chinensis and Rhodiola rosea exert an anti-stress effect on the HPA axis and reduce hypothalamic c-Fos expression in rats subjected to repeated stress

    DOI: 10.3892/etm.2015.2882
    Sample: Serum
  41. JOURNAL OF THE RENIN-ANGIOTENSIN-ALDOSTERONE SYSTEM (2019) IF: 1.356
    Combined but not single treatment with ethinylestradiol/levonorgestrel and spironolactone reduces plasminogen activator inhibitor-1 in insulin-resistant ovariectomised rats:

    DOI: 10.1177/1470320319895933
    Sample: Plasma
  42. JOURNAL OF GENETICS (2022) IF: 1.508
    Dietary intake of Spirulina platensis alters HSP70 gene expression profiles in the brain of rats in an experimental model of mixed stress

    DOI: 10.1007/s12041-022-01388-5
    Sample: serum
  43. Pharmacognosy Magazine (2018) IF: 1.525
    Naringin ameliorates doxorubicin-induced neurotoxicity In vitro and cognitive dysfunction In vivo

    DOI: 10.4103/pm.pm_364_17
    Sample: Serum
  44. Journal of Veterinary Research (2021) IF: 1.744
    The Preventive Role of Spirulina Platensis (Arthrospira Platensis) in Immune and Oxidative Insults in a Stress-induced Rat Model.

    DOI: 10.2478/jvetres-2021-0033
    Sample: serum
  45. Biological Research for Nursing (2020) IF: 1.789
    The Effect of Noise in the Intensive Care Unit on the Oxidative Stress Response in Rats:

    DOI: 10.1177/1099800420923397
    Sample: Plasma
  46. ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY (2018) IF: 1.806
    Blockade of mineralocorticoid receptor ameliorates oral contraceptive-induced insulin resistance by suppressing elevated uric acid and glycogen synthase kinase-3 instead of circulating mineralocorticoid

    DOI: 10.1080/13813455.2018.1509220

    PMID: 30318954

    		 Sample: Plasma
  47. IBRO Neuroscience Reports (2022)
    The Effect of Oxytocin and an Enriched Environment on Anxiety-Like Behaviour and Corticosterone Levels in a Prenatally Stressed Febrile Seizure Rat Model

    DOI: 10.1016/j.ibneur.2022.05.001
    Sample: plasma
  48. Beni-Suef University Journal of Basic and Applied Sciences (2018)
    Effect of intranasal insulin on peripheral glucose profile in dexamethasone-induced insulin resistance in Wistar rats

    DOI: 10.1016/j.bjbas.2018.06.003
    Sample: Cells
  49. IBRO Neuroscience Reports (2022)
    Bio-behavioural changes in treatment-resistant socially isolated FSL rats show variable or improved response to combined fluoxetine-olanzapine versus olanzapine treatment

    DOI: 10.1016/j.ibneur.2022.08.009

    PMID: 36204253

    		 Sample: plasma
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