Product Summary

Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Sensitivity	1.17 pg/mL
Detection Range	3.13-200 pg/mL
Sample Volume	50 μL
Total Assay Time	1 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat E2 in samples.No significant cross-reactivity or interference between Rat E2 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma, Urine, Saliva
Detection Method	Colorimetric method, ELISA, Competitive
Assay Type	Competitive-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	6 months

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat E2. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat E2 are added to the micro ELISA plate wells. Rat E2 in samples (or standards) competes with a fixed amount of E2 on the solid phase supporter for sites on the HRP linked detection antibody specific to E2. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Rat E2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Background

Estradiol, a hormone belonging to the group of estrogens, plays a crucial role in the female reproductive system and overall physiology. It is primarily produced in the ovaries and influences various aspects of female health and development.

Research Area

Cell Biology , Metabolism