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Cat.No.:E-OSEL-R0001
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Assay type | Competitive-ELISA |
Format | 96T/48T/24 |
Assay time | 1.5h |
Detection range | 3.13-200 pg/mL |
Sensitivity | 1.17 pg/mL |
Sample type &Sample volume | serum, plasma, urine, saliva; 50μL |
Specificity | This kit recognizes E2 in samples. No significant cross-reactivity or interference between E2 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of E2 concentrations in serum, plasma, urine, saliva. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 24T: 8 wells ×3 strips 96T*5: 5 plates, 96T |
2-8℃, 1 months |
Reference Standard | 96T: 2 vials
48T/24T: 1 vial 96T*5: 10 vials |
2-8℃, use the reconstituted standard within 24h |
Concentrated HRP Linked Detection Ab(100×) | 96T: 1 vial, 60 μL
48T/24T: 1 vial, 30 μL 96T*5: 5 vials, 60 μL |
2-8℃(Protect from light) |
Reference Standard & Sample Diluent | 96T/48T/24T: 1 vial, 20 mL 96T*5: 5 vials, 20 mL |
2-8℃ |
HRP Linked Ab Diluent | 96T/48T/24T: 1 vial, 14 mL 96T*5: 5 vials, 14 mL |
|
Concentrated Wash Buffer(25×) | 96T/48T/24T: 1 vial, 30 mL 96T*5: 5 vials, 30 mL |
|
Substrate Reagent | 96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL |
2-8℃(Protect from light) |
Stop Solution | 96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL |
2-8℃ |
Plate Sealer | 96T/48T/24T: 5 pieces 96T*5: 25 pieces |
|
Product Description | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level E2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level E2 were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 10.77 | 26.39 | 77.92 | 11.54 | 28.98 | 71.99 |
Standard deviation | 0.75 | 1.45 | 2.73 | 0.68 | 1.62 | 3.89 |
CV (%) | 6.98 | 5.51 | 3.51 | 5.89 | 5.59 | 5.40 |
The recovery of E2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 92-103 | 97 |
EDTA plasma(n=8) | 86-99 | 91 |
Samples were spiked with high concentrations of Rat E2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Synonyms | Oestradiol; E2; 17β-Estradiol; Estra-1,3,5(10)-triene-3,17β-diol |
Research Area | Cell biology, Metabolism |
PMID: 35422283 |
Sample: serum |
Sample: Serum |
PMID: 35883161 |
Sample: serum |
PMID: 36068745 |
Sample: Serum |
PMID: 36290602 |
Sample: serum |
PMID: 34030373 |
Sample: Serum |
Sample: serum |
Sample: serum |
Sample: cortex,hippocampus |
PMID: 35398386 |
Sample: serum |
PMID: 33126548 |
Sample: Serum |
DOI: 10.1530/JME-21-0095 |
|
PMID: 34270447 |
Sample: serum |
DOI: 10.1159/000457881 |
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Sample: Serum |
Sample: serum |
DOI: 10.1111/jcmm.16197 |
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PMID: 34346538 |
Sample: serum |
DOI: 10.1039/D1FO01744F |
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PMID: 35044398 |
Sample: serum |
PMID: 35175559 |
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PMID: 33638949 |
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PMID: 35668950 |
Sample: serum |
Sample: serum |
Sample: bone mesenchymal stem cell |
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DOI: 10.1530/JOE-19-0197 |
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Sample: serum |
Sample: serum |
DOI: 10.1039/C9FO02554E |
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PMID: 32441726 |
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PMID: 33328985 |
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PMID: 34229480 |
Sample: serum,testis |
PMID: 32540646 |
Sample: Serum,Tissue homogenate |
PMID: 36272568 |
Sample: ovarian granulosa cells |
PMID: 31476383 |
Sample: Cell sample |
DOI: 10.1111/jnc.14537 |
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Sample: Serum |
Sample: serum |
Sample: Cell culture supernatant |
Sample: serum |
PMID: 34656705 |
Sample: plasma |
PMID: 35429613 |
Sample: serum |
PMID: 36367482 |
Sample: serum |
DOI: 10.2147/DMSO.S285025 |
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Sample: Plasma |
PMID: 36041571 |
Sample: serum |
Sample: Serum |
Sample: serum |
PMID: 34972512 |
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PMID: 32311483 |
Sample: Serum |
DOI: 10.1002/ptr.6366 |
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Sample: serum |
Sample: serum |
DOI: 10.1530/REP-20-0316 |
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PMID: 33434152 |
Sample: Plasma |
DOI: 10.1530/REP-20-0451 |
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PMID: 33258800 |
Sample: Serum,Cell culture supernatant(ovarian granulosa c |
DOI: 10.1155/2018/3858209 |
|
Sample: serum |
DOI: 10.1071/RD21235 |
|
PMID: 35101163 |
Sample: serum |
Sample: Serum |
Sample: plasma |
DOI: 10.1155/2021/8874757 |
|
PMID: 33613687 |
Sample: Serum |
DOI: 10.1111/andr.12824 |
|
Sample: Tissue homogenate |
DOI: 10.1002/fsn3.2605 |
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Sample: serum |
PMID: 30483787 |
Sample: Serum |
PMID: 32945471 |
Sample: Serum |
PMID: 32945471 |
Sample: Serum |
Sample: Serum |
Sample: Serum |
Sample: Plasma |
PMID: 32892299 |
Sample: Serum |
Sample: serum |
DOI: 10.1111/rda.13172 |
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Sample: Serum |
PMID: 34879886 |
Sample: serum |
Sample: serum |
Sample: Serum,Tissue homogenate |
DOI: 10.1155/2020/6723204 |
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PMID: 32184896 |
Sample: Serum |
Sample: serum |
Sample: serum |
Sample: cerebral spinal fluid (CSF) |
Sample: Serum,Tissue homogenate |
Sample: Serum |