QuicKey Pro Rat IgG(Immunoglobulin G) ELISA Kit

Size	Price	Qty
96T	$ 520.00
48T	$ 416.00
24T	$ 150.00
96T*5	Inquire	/
96T*10	Inquire	/

Product Summary

Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Sensitivity	2.98 ng/mL
Detection Range	7.81-500 ng/mL
Sample Volume	50 μL
Total Assay Time	1 h 30 min
Reacitivity	Rat
Specificity	This kit recognizes Rat IgG in samples.No significant cross-reactivity or interference between Rat IgG and analogues was observed
Recovery	80%-120%
Sample Type	serum, plasma
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	6 months

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IgG. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat IgG are added to the micro ELISA plate wells. Rat IgG in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Rat IgG.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Rat IgG. The concentration of Rat IgG in the samples is then determined by comparing the OD of the samples to the standard curve.

Background

Immunoglobulin G (IgG) is an antibody, which is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged to form a Y type antibody monomer. Each IgG has two antigen-binding sites. IgG is the main antibody in blood and extracellular fluid that can control infection in human tissues. Plasma B cells produce and release IgG molecules. IgG is the only immunoglobulin that can pass through the human placenta, thereby protecting the fetus in the womb. IgG antibodies extracted from blood donor plasma can be used as intravenous immunoglobulins to treat immune deficiencies, autoimmune diseases, and infectious diseases.

Uniport ID	P20762
Research Area	Immunology

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QuicKey Pro Rat IgG(Immunoglobulin G) ELISA Kit (E-OSEL-R0005)

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