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QuicKey Pro Rat IgG(Immunoglobulin G) ELISA Kit

  • Cat.No.:E-OSEL-R0005

  • Reactivity: Rat

To Purchase E-OSEL-R0005

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $520
Qty:

QuicKey Pro Series

Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T/24
Assay time 1.5h
Detection range 7.81-500 ng/mL
Sensitivity 2.98 ng/mL
Sample type &Sample volume serum and plasma; 50μL
Specificity This kit recognizes IgG in samples. No significant cross-reactivity or interference between IgG and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of IgG concentrations in serum and plasma.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IgG. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat IgG are added to the micro ELISA plate wells. Rat IgG in samples (or standards) combines with the coated antibody and HRP linked detection antibody specific to IgG. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Rat IgG in the samples is then determined by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the table.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
24T: 8 wells ×3 strips
96T*5: 5 plates, 96T
2-8℃, 1 months
Reference Standard 96T: 2 vials
48T/24T: 1 vial
96T*5: 10 vials
2-8℃, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100×) 96T: 1 vial, 60 μL
48T/24T: 1 vial, 30 μL
96T*5: 5 vials, 60 μL
2-8℃(Protect from light)
Reference Standard & Sample Diluent 96T/48T/24T: 1 vial, 20 mL
96T*5: 5 vials, 20 mL
2-8℃
HRP Linked Ab Diluent 96T/48T/24T: 1 vial, 14 mL
96T*5: 5 vials, 14 mL
Concentrated Wash Buffer(25×) 96T/48T/24T: 1 vial, 30 mL
96T*5: 5 vials, 30 mL
Substrate Reagent 96T/48T/24T: 1 vial, 10 mL
96T*5: 5 vials, 10 mL
2-8℃(Protect from light)
Stop Solution 96T/48T/24T: 1 vial, 10 mL
96T*5: 5 vials, 10 mL
2-8℃
Plate Sealer 96T/48T/24T: 5 pieces
96T*5: 25 pieces
Product Description 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level IgG were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level IgG were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 23.38 68.80 230.89 21.47 74.91 214.43
Standard deviation 1.42 3.51 7.37 1.34 3.26 6.65
CV (%) 6.08 5.10 3.19 6.26 4.35 3.10

Recovery

The recovery of IgG spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-102 94
EDTA plasma(n=8) 85-97 90

Linearity

Samples were spiked with high concentrations of Rat IgG and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsImmunoglobulin G,IgG
Research AreaImmunology

Assay Procedures

Citations

  1. Frontiers in Bioengineering and Biotechnology (2022) IF: 6.064
    Safety and biodistribution of exosomes derived from human induced pluripotent stem cells.

    DOI: 10.3389/fbioe.2022.949724

    Sample: Blood
  2. Journal of Zhejiang University-SCIENCE B (2019) IF: 1.879
    Improvement in post-partum uterine involution in rats treated with Apios americana

    DOI: 10.1631/jzus.B1800475

    Sample: Serum
  3. Dose-Response (2022) IF: 2.623
    Pharmacological Role of Capparis decidua (Forssk.) Edgew in Preventing Cyclophosphamide-induced Myelosuppression and Modulating Innate and Adaptive Immune Response:

    DOI: 10.1177/15593258221123672

    Sample: Serum
  4. Scientific Reports (2016) IF: 5.228
    mTOR Pathway

    DOI: 10.1038/srep31771

    Sample: Urine
  5. FASEB JOURNAL (2018) IF: 5.595
    A novel immunodeficient rat model supports human lung cancer xenografts

    DOI: 10.1096/fj.201800102RR

    Sample: Serum
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