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QuicKey Pro Rat Pg(Progesterone) ELISA Kit

Cat.No.:E-OSEL-R0007
Reactivity: Rat

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96T
48T
24T
96T*5
96T*10

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Product Details

Properties

Assay type	Competitive-ELISA
Format	96T/48T/24
Assay time	1.5h
Detection range	15.63-1000 pg/mL
Sensitivity	7.01 pg/mL
Sample type &Sample volume	serum and plasma; 50μL
Specificity	This kit recognizes Pg in samples. No significant cross-reactivity or interference between Pg and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Pg concentrations in serum and plasma.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat Pg. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat Pg are added to the micro ELISA plate wells. Rat Pg in samples (or standards) competes with a fixed amount of Pg on the solid phase supporter for sites on the HRP linked detection antibody specific to Pg. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Rat Pg in the samples is then determined by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the table.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips 24T: 8 wells ×3 strips 96T*5: 5 plates, 96T	2-8℃, 1 months
Reference Standard	96T: 2 vials 48T/24T: 1 vial 96T*5: 10 vials	2-8℃, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100×)	96T: 1 vial, 60 μL 48T/24T: 1 vial, 30 μL 96T*5: 5 vials, 60 μL	2-8℃(Protect from light)
Reference Standard & Sample Diluent	96T/48T/24T: 1 vial, 20 mL 96T*5: 5 vials, 20 mL	2-8℃
HRP Linked Ab Diluent	96T/48T/24T: 1 vial, 14 mL 96T*5: 5 vials, 14 mL
Concentrated Wash Buffer(25×)	96T/48T/24T: 1 vial, 30 mL 96T*5: 5 vials, 30 mL
Substrate Reagent	96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL	2-8℃(Protect from light)
Stop Solution	96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL	2-8℃
Plate Sealer	96T/48T/24T: 5 pieces 96T*5: 25 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Pg were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Pg were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	51.82	153.05	376.37	50.57	167.62	390.77
Standard deviation	3.26	7.61	17.39	2.91	8.55	19.58
CV (%)	6.30	4.97	4.62	5.76	5.10	5.01

Recovery

The recovery of Pg spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	91-101	96
EDTA plasma(n=8)	94-107	101

Linearity

Samples were spiked with high concentrations of Rat Pg and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	Progesterone，Pg
Research Area	Signal Transduction

Assay Procedures

Citations

CARBOHYDRATE POLYMERS (2022) IF: 9.381

Butylated starch alleviates polycystic ovary syndrome by stimulating the secretion of peptide tyrosine-tyrosine and regulating faecal microbiota

DOI: 10.1016/j.carbpol.2022.119304
PMID: 35422283	Sample: serum

ENVIRONMENTAL POLLUTION (2023) IF: 9.988

Paternal genetic intergenerational and transgenerational effects of cadmium exposure on hormone synthesis disorders in progeny ovarian granulosa cells

DOI: 10.1016/j.envpol.2023.121175
	Sample: Serum

Antioxidants (2022) IF: 7.675

Kelulut Honey Ameliorates Oestrus Cycle, Hormonal Profiles, and Oxidative Stress in Letrozole-Induced Polycystic Ovary Syndrome Rats

DOI: 10.3390/antiox11101879
PMID: 36290602	Sample: serum

International Journal of Nanomedicine (2021) IF: 6.4

Development and Evaluation of Curcumin Encapsulated Self-assembled Nanoparticles as Potential Remedial Treatment for PCOS in a Female Rat Model

DOI: 10.2147/IJN.S302161
PMID: 34531655	Sample: serum

CHEMICO-BIOLOGICAL INTERACTIONS (2023) IF: 5.168

Protective effect of eicosapentaenoic acid against estradiol valerate-induced endometrial hyperplasia via modulation of NF-κB/HIF-1α/VEGF signaling pathway in rats

DOI: 10.1016/j.cbi.2023.110399
	Sample: serum

Food & Function (2022) IF: 5.396

Lactiplantibacillus plantarum CCFM1019 attenuate polycystic ovary syndrome through butyrate dependent gut–brain mechanism

DOI: 10.1039/D1FO01744F
PMID: 35044398	Sample: serum

Frontiers in Pharmacology (2022) IF: 5.988

Andrographolide exerted anti-inflammatory effects thereby reducing sex hormone synthesis in LPS-induced female rats, but had no effect on hormone production in healthy ones.

DOI: 10.3389/fphar.2022.980064
	Sample: serum

BIOLOGY OF REPRODUCTION (2022) IF: 4.161

Role of progesterone on dexamethasone-induced alterations in placental vascularization and progesterone receptors in rats

DOI: 10.1093/biolre/ioac192
	Sample: serum

Frontiers in Pharmacology (2020) IF: 4.225

Chemical Fingerprint Analysis and Ultra-Performance Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry-Based Metabolomics Study of the Protective Effect of Buxue Yimu Granule in Medical-Induced Incomplete Abortion Rats

DOI: 10.3389/fphar.2020.578217
PMID: 33328985	Sample: serum

MOLECULES (2021) IF: 4.412

The Physicochemical Characteristics of Gelam Honey and Its Outcome on the Female Reproductive Tissue of Sprague–Dawley Rats: A Preliminary Study

DOI: 10.3390/molecules26113346
	Sample: serum

TOXICOLOGY (2022) IF: 4.571

A metabolomic approach to study triptolide-induced ovarian damage in rats

DOI: 10.1016/j.tox.2022.153351
PMID: 36272568	Sample: ovarian granulosa cells

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2020) IF: 4.872

Cadmium exposure during prenatal development causes progesterone disruptors in multiple generations via steroidogenic enzymes in rat ovarian granulosa cells

DOI: 10.1016/j.ecoenv.2020.110765
	Sample: Cell culture supernatant

REPRODUCTIVE TOXICOLOGY (2022) IF: 3.143

Bisphenol A exposure advances puberty onset by changing Kiss1 expression firstly in arcuate nucleus at juvenile period in female rats

DOI: 10.1016/j.reprotox.2022.04.005
PMID: 35429613	Sample: serum

NEUROPEPTIDES (2022) IF: 3.152

Effects of intracerebroventricular and intravenous administration of Kisspeptin-54 and Gonadotropin-releasing hormone agonist in rats with ovarian hyperstimulation

DOI: 10.1016/j.npep.2022.102298
PMID: 36367482	Sample: serum

BEHAVIOURAL BRAIN RESEARCH (2022) IF: 3.352

Postpartum depression in rats causes poor maternal care and neurochemical alterations on dams and long-lasting impairment in sociability on the offspring

DOI: 10.1016/j.bbr.2022.114082
PMID: 36041571	Sample: serum

FOOD AND CHEMICAL TOXICOLOGY (2019) IF: 3.775

Cadmium exposure during prenatal development causes testosterone disruption in multigeneration via SF-1 signaling in rats

DOI: 10.1016/j.fct.2019.110897
PMID: 31654709	Sample: Serum

REPRODUCTIVE BIOMEDICINE ONLINE (2021) IF: 3.828

Progesterone partially recovers placental glucose transporters in dexamethasone induced intrauterine growth restriction

DOI: 10.1016/j.rbmo.2021.10.016
	Sample: serum

Evidence-based Complementary and Alternative Medicine (2018) IF: 2.064

Research on Establishment of Abnormal Phlegmatic Syndrome with Premature Ovarian Failure Rat Model and Effects of Balgham Munziq Treatment

DOI: 10.1155/2018/3858209
	Sample: serum

MOLECULAR BIOLOGY REPORTS (2022) IF: 2.316

The effect of progesterone administration on the expression of metastasis tumor antigens (MTA1 and MTA3) in placentas of normal and dexamethasone-treated rats

DOI: 10.1007/s11033-021-07005-5
PMID: 35037193	Sample: serum

Evidence-based Complementary and Alternative Medicine (2022) IF: 2.65

Shoutai Wan Improves Embryo Survival by Regulating Aerobic Glycolysis of Trophoblast Cells in a Mouse Model of Recurrent Spontaneous Abortion

DOI: 10.1155/2022/8251503
PMID: 36212974	Sample: serum

Food Science & Nutrition (2021) IF: 2.863

Metabolomics study of Angelica sinensis (Oliv.) Diels on the abnormal uterine bleeding rats by ultra-performance liquid chromatography–quadrupole–time-of-flight mass spectrometry analysis

DOI: 10.1002/fsn3.2605
	Sample: serum

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2019) IF: 2.928

Expression of FKBP52 in the ovaries of PCOS rats

DOI: 10.3892/ijmm.2018.3998
PMID: 30483787	Sample: Serum

REPRODUCTION IN DOMESTIC ANIMALS (2018) IF: 1.422

Methylation pattern variation between goats and rats during the onset of puberty

DOI: 10.1111/rda.13172
	Sample: Serum

Toxicology Research (2019) IF: 1.593

Di-(2-ethylhexyl) phthalate (DEHP) inhibits steroidogenesis and induces mitochondria-ROS mediated apoptosis in rat ovarian granulosa cells

DOI: 10.1039/C8TX00263K
PMID: 31160972	Sample: Cell culture medium

Journal of Zhejiang University-SCIENCE B (2019) IF: 1.879

Improvement in post-partum uterine involution in rats treated with Apios americana

DOI: 10.1631/jzus.B1800475
	Sample: serum

Veterinary World (2022)

Potential use of Indonesian basil (Ocimum basilicum) maceration to increase estradiol and progesterone synthesis and secretion to improve prenatal growth of offspring using female albino rats as an animal model

DOI: 10.14202/vetworld.2022.1197-1207
	Sample: serum

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Cat. No	E-OSEL-R0007
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