Product Summary

Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Sensitivity	0.07 ng/mL
Detection Range	0.16-10 ng/mL
Sample Volume	50 μL
Total Assay Time	1 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat T in samples.No significant cross-reactivity or interference between Rat T and analogues was observed
Recovery	80%-120%
Sample Type	serum, plasma
Detection Method	Colorimetric method, ELISA, Competitive
Assay Type	Competitive-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	6 months

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat T. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat T are added to the micro ELISA plate wells. Rat T in samples (or standards) competes with a fixed amount of T on the solid phase supporter for sites on the HRP linked detection antibody specific to T. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Rat T in the samples is then determined by comparing the OD of the samples to the standard curve.

Background

Testosterone is a male hormone primarily produced by the testes and is crucial for male physiological function. It not only participates in the generation of sperm, but also involves various physiological processes, including but not limited to sexual desire, sperm quality, and physical health. In some specific clinical studies, testosterone replacement therapy has been used to treat male patients with age-related low testosterone levels, who may experience abnormal testosterone levels due to various reasons such as drug side effects, disease, or lifestyle changes

Research Area

Signal Transduction , Signal Transduction , Epigenetics And Nuclear Signaling , Developmental Biology