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Rabbit TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit

  • Cat.No.:E-EL-RB0011

  • Reactivity: Rabbit

To Purchase E-EL-RB0011

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $588
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rabbit TNF-α in samples. No significant cross-reactivity or interference between Rabbit TNF-α and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rabbit TNF-α concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit TNF-α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit TNF-α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit TNF-α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rabbit TNF-α. You can calculate the concentration of Rabbit TNF-α in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rabbit TNF-α were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rabbit TNF-α were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 49.73 154.44 395.12 47.58 159.52 432.48
Standard deviation 2.85 6.49 13.00 2.45 8.87 22.79
CV (%) 5.73 4.20 3.29 5.15 5.56 5.27

Recovery

The recovery of Rabbit TNF-α spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 96-109 101
EDTA plasma (n=8) 92-109 99
Cell culture media (n=8) 88-100 93

Linearity

Samples were spiked with high concentrations of Rabbit TNF-α and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P04924
SynonymsDIF, TNF-alpha, TNFA, TNFSF2

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Vaccines (2020) IF: 4.086
    An Antibody Persistent and Protective Two rSsCLP-Based Subunit Cocktail Vaccine against Sarcoptes scabiei in a Rabbit Model

    DOI: 10.3390/vaccines8010129

    PMID: 32187979

    Sample: Serum
  2. World Journal of Stem Cells (2019) IF: 3.231
    Anti-osteoarthritis effect of a combination treatment with human adipose tissue-derived mesenchymal stem cells and thrombospondin 2 in rabbits

    DOI: 10.4252/wjsc.v11.i12.1115

    PMID: 31875872

    Sample: Synovia
  3. BioImpacts: BI (2019) IF: 3.475
    In vitro and in vivo evaluation of a dextran-graft-polybutylmethacrylate copolymer coated on CoCr metallic stent

    DOI: 10.15171/bi.2019.04

    PMID: 30788257

    Sample: Serum
  4. Toxins (2018) IF: 3.273
    Establishment of a New Zealand White Rabbit Model for Lethal Toxin (LT) Challenge and Efficacy of Monoclonal Antibody 5E11 in the LT-Challenged Rabbit Model.

    DOI: 10.3390/toxins10070289

    PMID: 30002351

    Sample: Serum
  5. PloS one (2020) IF: 2.74
    Testicular toxicity following chronic codeine administration is via oxidative DNA damage and up-regulation of NO/TNF-α and caspase 3 activities

    DOI: 10.1371/journal.pone.0224052

    PMID: 32168344

    Sample: Serum,Tissue homogenate
  6. Current eye research (2019) IF: 2.12
    The Efficiency of Cyclosporine A-Eluting Contact Lenses for the Treatment of Dry Eye

    DOI: 10.1080/02713683.2018.1563702

    PMID: 30580651

    Sample: Tissue homogenate
  7. Biomedicine & Pharmacotherapy (2018) IF: 2.759
    Oleanolic acid protects against pathogenesis of atherosclerosis, possibly via FXR-mediated angiotensin (Ang)-(1-7) upregulation.

    DOI: 10.1016/j.biopha.2017.11.151

    PMID: 29793333

    Sample: Cell culture supernatant,Serum
  8. Molecular Vision (2017) IF: 2.057
    Inhibitory Effects Of S100A4 Gene Silencing On Alkali Burn-Induced Corneal Neovascularization: An In Vivo Study.

    PMID: 28479848

    Sample: Aqueous humor
  9. International Journal of Molecular Medicine (2016) IF: 2.341
    Hypothermic Machine Perfusion Increases A20 Expression Which Protects Renal Cells Against Ischemia/Reperfusion Injury By Suppressing Inflammation, Apoptosis And Necroptosis.

    DOI: 10.3892/ijmm.2016.2586

    PMID: 27177159

    Sample: Tissue homogenate
  10. Artificial Organs (2016) IF: 2.403
    Hypothermic Machine Perfusion Reduced Inammatory Reaction By Downregulating The Expression Of Matrix Metalloproteinase 9 In A Reperfusion Model Of Donation After Cardiac Death.

    DOI: 10.1111/aor.12658

    PMID: 26813475

    Sample: Serum
  11. World neurosurgery (2019) IF: 1.924
    Neuroprotective Effects of Tocilizumab on Experimentally-Induced Spinal Cord Ischemia-Reperfusion Injury

    DOI: 10.1016/j.wneu.2018.12.069

    PMID: 30593966

    Sample: Plasma,Tissue homogenate
  12. Theriogenology (2017) IF: 1.986
    Effects Of Local Lipopolysaccharide Administration On The Expression Of Toll-Like Receptor 4 And Pro-Inflammatory Cytokines In Uterus And Oviduct Of Rabbit Does.

    DOI: 10.1016/j.theriogenology.2017.10.046

    PMID: 29161657

    Sample: Plasma
  13. Romanian Neurosurgery (2020)
    The efficacy of Adal?mumab on experimentally induced spinal cord ischemia-reperfusion injury

    DOI: 10.33962/roneuro-2020-056

    PMID: 99999999

    Sample: Plasma,Tissue homogenate
  14. Journal of Obstetrics and Gynaecology (2018) IF: 0.629
    Stress response to ovariohysterectomy in rabbits: role of anaesthesia and surgery.

    DOI: 10.1080/01443615.2017.1400523

    PMID: 29519176

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