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Rat AChE(Acetylcholinesterase) ELISA Kit

  • Cat.No.:E-EL-R0355

  • Reactivity: Rat

To Purchase E-EL-R0355

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.78-50 ng/mL
Sensitivity 0.47 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat AChE in samples. No significant cross-reactivity or interference between Rat AChE and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat AChE concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat AChE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat AChE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat AChE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat AChE. You can calculate the concentration of Rat AChE in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat AChE were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat AChE were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 2.43 7.13 17.70 2.43 7.84 16.22
Standard deviation 0.13 0.29 0.91 0.13 0.42 0.57
CV (%) 5.35 4.07 5.14 5.35 5.36 3.51


The recovery of Rat AChE spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-103 97
EDTA plasma (n=8) 89-99 94
Cell culture media (n=8) 90-106 97


Samples were spiked with high concentrations of Rat AChE and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsACEE, ARACHE, N-ACHE, YT, acetylhydrolase
Research AreaCancer, Neuroscience

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


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View more details about our Publication Reward >>

    Effect of bisphenol F on sexual performance and quality of offspring in Male Wistar rats

    DOI: 10.1016/j.ecoenv.2022.114079

    PMID: 36115153

    Sample: plasma
    Protective Role of Acetate Against Depressive-Like Behaviour Associated with Letrozole-Induced PCOS Rat Model: Involvement of HDAC2 and DNA Methylation

    DOI: 10.1007/s12035-022-03074-0

    PMID: 36269541

    Sample: PFC,hippocampal tissues
  3. Frontiers in Pharmacology (2022) IF: 5.988
    Integrated comparative metabolomics and network pharmacology approach to uncover the key active ingredients of Polygonati rhizoma and their therapeutic potential for the treatment of Alzheimer's disease.

    DOI: 10.3389/fphar.2022.934947

    Sample: PC12 cell
  4. Toxics (2019) IF: 4.146
    Protective Effects of Centella asiatica on Cognitive Deficits Induced by D-gal/AlCl3 via Inhibition of Oxidative Stress and Attenuation of Acetylcholinesterase Level

    DOI: 10.3390/toxics7020019

    PMID: 30935005

    Sample: Tissue homogenate
    Naproxen as a potential candidate for promoting rivastigmine anti-Alzheimer activity against aluminum chloride-prompted Alzheimer's-like disease in rats; neurogenesis and apoptosis modulation as a possible underlying mechanism

    DOI: 10.1016/j.ejphar.2021.174695

    PMID: 34914971

    Sample: hippocampal tissue
  6. MOLECULES (2022) IF: 4.927
    Modulation of High-Fat Diet-Induced Brain Oxidative Stress by Ferulate-Rich Germinated Brown Rice Ethyl Acetate Extract

    DOI: 10.3390/molecules27154907

    PMID: 35956857

    Sample: brain
  7. NEUROSCIENCE LETTERS (2022) IF: 3.046
    Differential effects of voluntary and forced exercise trainings on spatial learning ability and hippocampal biomarkers in aged female rats

    DOI: 10.1016/j.neulet.2022.136499

    PMID: 35121056

    Sample: hippocampus
  8. Processes (2023) IF: 3.352
    Memory-Improving Activity of the Flower Extract from Chrysanthemum boreale (Makino) Maskino in Scopolamine-Treated Rodents

    DOI: 10.3390/pr11010159

    Sample: serum
  9. LIFE SCIENCES (2020) IF: 3.647
    Attenuation of chronic arsenic neurotoxicity via melatonin in male offspring of maternal rats exposed to arsenic during conception: Involvement of oxidative DNA damage and inflammatory signaling cascades

    DOI: 10.1016/j.lfs.2020.118876

    PMID: 33310035

    Sample: brine tissue
    Biochemical and molecular alterations in freshwater mollusks as biomarkers for petroleum product, domestic heating oil

    DOI: 10.1016/j.ecoenv.2018.04.006

    Sample: Tissue homogenate
    Restraint Stress Exacerbates Apoptosis in a 6-OHDA Animal Model of Parkinson Disease

    DOI: 10.1007/s12640-022-00630-3

    Sample: striatal tissue
    Alteration of the α5 GABA receptor and 5HTT lead to cognitive deficits associated with major depressive-like behaviors in a 14-day combined stress rat model

    DOI: 10.1080/00207454.2021.2019033

    PMID: 34937496

    Sample: hippocampus,Prefrontal cortex(PFC)
  13. NEUROLOGICAL RESEARCH (2023) IF: 2.529
    The effect of the calcium channel blocker nimodipine on hippocampal BDNF/Ach levels in rats with experimental cognitive impairment

    DOI: 10.1080/01616412.2022.2164452

    Sample: Hippocampal,prefrontal cortex
  14. Journal of Experimental Zoology Part A-Ecological and Integrative Physiology (2022) IF: 2.693
    Molecular mechanisms underlying ameliorative impact of melatonin against age-dependent chronic arsenic toxicity in rats' brains

    DOI: 10.1002/jez.2603

    Sample: brain
    Possible combined effect of perindopril and Azilsartan in an experimental model of dementia in rats

    DOI: 10.1016/j.jsps.2020.03.009

    Sample: Tissue homogenate
  16. Advances in Traditional Medicine (2022)
    Nootropic effect of Syzygium polyanthum (Wight) Walp leaf extract in chronic cerebral hypoperfusion rat model via cholinergic restoration: a potential therapeutic agent for dementia

    DOI: 10.1007/s13596-022-00653-3

    Sample: brain
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