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Rat ADP/Acrp30(Adiponectin) ELISA Kit

Citations(36)

  • Cat.No.:E-EL-R3012

  • Reactivity: Rat
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Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 1.56-100 ng/mL
Sensitivity 0.94 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat ADP/Acrp30 in samples. No significant cross-reactivity or interference between Rat ADP/Acrp30 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat ADP/Acrp30 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ADP/Acrp30. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ADP/Acrp30 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ADP/Acrp30, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ADP/Acrp30. You can calculate the concentration of Rat ADP/Acrp30 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat ADP/Acrp30 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat ADP/Acrp30 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 4.91 10.17 40.58 4.17 11.09 42.27
Standard deviation 0.29 0.51 1.83 0.23 0.53 1.88
CV (%) 5.91 5.01 4.51 5.52 4.78 4.45

Recovery

The recovery of Rat ADP/Acrp30 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-105 98
EDTA plasma (n=8) 90-104 98
Cell culture media (n=8) 85-93 90

Linearity

Samples were spiked with high concentrations of Rat ADP/Acrp30 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsAcrp30, GBP28, ACDC, APM1, ADPN, AdipoQ, ADIPQTL1
Research AreaCancer, Cardiovascular, Metabolism, Neuroscience, Signal transduction, Stem cells

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Antioxidants (2020) IF: 6.313
    Protective Effects of a Discontinuous Treatment with Alpha-Lipoic Acid in Obesity-Related Heart Failure with Preserved Ejection Fraction, in Rats

    DOI: 10.3390/antiox9111073

    PMID: 33142857

    		 Sample: Plasma
  2. Antioxidants (2021) IF: 6.313
    Hepatoprotective Effect of Bee Bread in Metabolic Dysfunction-Associated Fatty Liver Disease (MAFLD) Rats: Impact on Oxidative Stress and Inflammation

    DOI: 10.3390/antiox10122031

    PMID: 34943134

    		 Sample: serum
  3. Antioxidants (2021) IF: 6.313
    Anti-Atherogenic Effects of Orlistat on Obesity-Induced Vascular Oxidative Stress Rat Model

    DOI: 10.3390/antiox10020251

    PMID: 33562069

    		 Sample: Serum
  4. Pharmaceutics (2022) IF: 6.525
    The Anti-Obesity Potential of Superparamagnetic Iron Oxide Nanoparticles against High-Fat Diet-Induced Obesity in Rats: Possible Involvement of Mitochondrial Biogenesis in the Adipose Tissues

    DOI: 10.3390/pharmaceutics14102134

    PMID: 36297569

    		 Sample: serum
  5. Pharmaceutics (2022) IF: 6.525
    l-Arginine Induces White Adipose Tissue Browning—A New Pharmaceutical Alternative to Cold

    DOI: 10.3390/pharmaceutics14071368
    Sample: Serum
  6. JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY (2022) IF: 5.011
    Acetate: A therapeutic candidate against renal disorder in a rat model of polycystic ovarian syndrome

    DOI: 10.1016/j.jsbmb.2022.106179

    PMID: 36150640

    		 Sample: plasma
  7. Frontiers in Cellular and Infection Microbiology (2021) IF: 5.293
    Alleviation Effects of GQD, a Traditional Chinese Medicine Formula, on Diabetes Rats Linked to Modulation of the Gut Microbiome

    DOI: 10.3389/fcimb.2021.740236
    Sample: serum
  8. Frontiers in Pharmacology (2021) IF: 5.811
    Hydroethanolic Extract of A. officinarum Hance Ameliorates Hypertension and Causes Diuresis in Obesogenic Feed-Fed Rat Model

    DOI: 10.3389/fphar.2021.670433
    Sample: serum
  9. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021) IF: 5.924
    Metabolic Disturbances in Rat Sublines with Constitutionally Altered Serotonin Homeostasis

    DOI: 10.3390/ijms22105400

    PMID: 34065591

    		 Sample: Tissue homogenate
  10. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM (2021) IF: 4.31
    Bee bread mitigates downregulation of steroidogenic genes, decreased spermatogenesis, and epididymal oxidative stress in male rats fed with high-fat diet

    DOI: 10.1152/ajpendo.00093.2021

    PMID: 34229480

    		 Sample: serum,testis
  11. SAUDI PHARMACEUTICAL JOURNAL (2023) IF: 4.562
    6-gingerol ameliorates weight gain and insulin resistance in metabolic syndrome rats by regulating adipocytokines

    DOI: 10.1016/j.jsps.2023.01.003
    Sample: serum,adipose tissues
  12. PSYCHONEUROENDOCRINOLOGY (2022) IF: 4.693
    Increased growth hormone secretagogue receptor-1a (GHSR-1a) in hypothalamus during olanzapine treatment in rats

    DOI: 10.1016/j.psyneuen.2022.105862

    PMID: 35835020

    		 Sample: Serum
  13. JOURNAL OF NUTRITIONAL BIOCHEMISTRY (2020) IF: 4.873
    Fish oil and corn oil induced differential effect on beiging of visceral and subcutaneous white adipose tissue in high-fat-diet-induced obesity

    DOI: 10.1016/j.jnutbio.2020.108458
    Sample: Serum
  14. NUTRITION (2022) IF: 4.893
    Acetate circumvents impaired metabolic switch in skeletal muscle of letrozole-induced PCOS rat model by suppression of PDK4/NLRP3

    DOI: 10.1016/j.nut.2022.111914
    Sample: plasma
  15. MOLECULES (2023) IF: 4.927
    The Anti-Obesity and Anti-Steatotic Effects of Chrysin in a Rat Model of Obesity Mediated through Modulating the Hepatic AMPK/mTOR/lipogenesis Pathways

    DOI: 10.3390/molecules28041734
    Sample: Serum
  16. PeerJ (2022) IF: 3.061
    Effects of combination of obesity, diabetes, and hypoxia on inflammatory regulating genes and cytokines in rat pancreatic tissues and serum

    DOI: 10.7717/peerj.13990

    PMID: 36213511

    		 Sample: serum
  17. ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY (2020) IF: 3.292
    Nicotine-mediated upregulation of microRNA-141 expression determines adipokine-intervened insulin resistance

    DOI: 10.1016/j.etap.2020.103506

    PMID: 33002592

    		 Sample: Serum
  18. TOXICOLOGY AND APPLIED PHARMACOLOGY (2020) IF: 3.347
    Low dose spironolactone-mediated androgen-adiponectin modulation alleviates endocrine-metabolic disturbances in letrozole-induced PCOS

    DOI: 10.1016/j.taap.2020.115381

    PMID: 33359182

    		 Sample: Plasma
  19. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2019) IF: 3.571
    Antiobesity Effect of Flaxseed Polysaccharide via Inducing Satiety due to Leptin Resistance Removal and Promoting Lipid Metabolism through the AMP-Activated Protein Kinase (AMPK) Signaling Pathway

    DOI: 10.1021/acs.jafc.9b02434
    Sample: serum
  20. JOURNAL OF FOOD BIOCHEMISTRY (2022) IF: 3.654
    Curcumin nanoemulsion counteracts hepatic and cardiac complications associated with high-fat/high-fructose diet in rats

    DOI: 10.1111/jfbc.14442

    PMID: 36165438

    		 Sample: serum
  21. PHYTOTHERAPY RESEARCH (2019) IF: 3.766
    Synergistic effects of the capsaicinoid nonivamide and rosuvastatin on obesity‐related endothelial dysfunction in rat fed a high‐fat diet

    DOI: 10.1002/ptr.6369
    Sample: blood
  22. NUTRITION RESEARCH (2022) IF: 3.876
    Pine nut oil supplementation alleviates the obesogenic effects in high-fat diet induced obese rats: A comparative study between epididymal and retroperitoneal adipose tissue.

    DOI: 10.1016/j.nutres.2022.07.003
    Sample: serum
  23. NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY (2020) IF: 2.05
    Possible mechanisms mediating the protective effect of cilostazol in L-arginine induced acute pancreatitis in rats: role of cGMP, cAMP, and HO-1

    DOI: 10.1007/s00210-020-01897-z

    PMID: 32424476

    		 Sample: Serum
  24. ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY (2019) IF: 2.11
    Evaluating the effects of Juglans regia L. extract on hyperglycaemia and insulin sensitivity in experimental type 2 diabetes in rat

    DOI: 10.1080/13813455.2019.1668018
    Sample: Plasma
  25. CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2018) IF: 2.21
    Combined treatment with interleukin-1 and tumor necrosis factor-alpha antagonists improve type 2 diabetes in rats

    DOI: 10.1139/cjpp-2017-0769
    Sample: Serum
  26. SOUTH AFRICAN JOURNAL OF BOTANY (2021) IF: 2.315
    Investigation of therapeutic potential of three endemic Cirsium species for global health problem obesity

    DOI: 10.1016/j.sajb.2021.05.004
    Sample: Serum
  27. Journal of Developmental Origins of Health and Disease (2020) IF: 2.456
    Neonatal zingerone protects against the development of high-fructose diet-induced metabolic syndrome in adult Sprague-Dawley rats

    DOI: 10.1017/S2040174420000525
    Sample: plasma
  28. JOURNAL OF BIOMEDICAL SCIENCE (2015) IF: 2.763
    High fat diet-induced inflammation and oxidative stress are attenuated by N-acetylneuraminic acid in rats

    DOI: 10.1186/s12929-015-0211-6
    Sample: Serum
  29. Andrology (2020) IF: 2.86
    Orlistat attenuates obesity‐induced decline in steroidogenesis and spermatogenesis by up‐regulating steroidogenic genes

    DOI: 10.1111/andr.12824
    Sample: Tissue homogenate
  30. MOLECULES (2017) IF: 2.861
    Two Sulfur Glycoside Compounds Isolated from Lepidium apetalum Willd Protect NRK52e Cells against Hypertonic-Induced Adhesion and Inflammation by Suppressing the MAPK Signaling Pathway and RAAS

    DOI: 10.3390/molecules22111956
    Sample: Cell culture supernatant
  31. Biomed Research International (2015) IF: 1.579
    N-Acetylneuraminic Acid Supplementation Prevents High Fat Diet-Induced Insulin Resistance in Rats through Transcriptional and Nontranscriptional Mechanisms

    DOI: 10.1155/2015/602313
    Sample: Serum
  32. BIOLOGIA (2022) IF: 1.653
    The effects of β-escin on inflammation, oxidative stress and Langerhans islet cells in high-fat diet and streptozotocin injection induced experimental type-2 diabetes model

    DOI: 10.1007/s11756-022-01266-6
    Sample: serum
  33. Toxicology Reports (2021)
    Sildenafil augments fetal weight and placental adiponectin in gestational testosterone-induced glucose intolerant rats

    DOI: 10.1016/j.toxrep.2021.06.011
    Sample: Placenta
  34. Heliyon (2021)
    Sildenafil ameliorates leptin resistance and normalizes lipid handling in the hypothalamic and adipose tissues of testosterone-exposed pregnant rats

    DOI: 10.1016/j.heliyon.2021.e07574
    Sample: plasma
  35. Biomedical Reports (2017)
    Metabolic effects of Crocus sativus and protective action against non-alcoholic fatty liver disease in diabetic rats

    DOI: 10.3892/br.2017.884
    Sample: serum
  36. Asian Pacific Journal of Tropical Medicine (2016) IF: 0.841
    Changes of adipocytokine expression after diabetic rats received sitagliptin and the molecular mechanism

    DOI: 10.1016/j.apjtm.2016.07.014
    Sample: Serum
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