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Rat Bcl-2(B-cell Lymphoma/Leukemia 2) ELISA Kit

Citations(25)

  • Cat.No.:E-EL-R0096

  • Reactivity: Rat
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Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.16-10 ng/mL
Sensitivity 0.10 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat Bcl-2 in samples. No significant cross-reactivity or interference between Rat Bcl-2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat Bcl-2 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat Bcl-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat Bcl-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat Bcl-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat Bcl-2. You can calculate the concentration of Rat Bcl-2 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat Bcl-2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat Bcl-2 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.50 1.40 3.80 0.50 1.40 4.00
Standard deviation 0.03 0.06 0.17 0.03 0.07 0.20
CV (%) 6.00 4.29 4.47 6.00 5.00 5.00

Recovery

The recovery of Rat Bcl-2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-99 93
EDTA plasma (n=8) 86-98 91
Cell culture media (n=8) 93-108 99

Linearity

Samples were spiked with high concentrations of Rat Bcl-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsBcl2, PPP1R50
Research AreaCancer, Cell Biology, Metabolism, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. International Journal of Nanomedicine (2021) IF: 6.4
    Neuroprotective Efficiency of Prodigiosins Conjugated with Selenium Nanoparticles in Rats Exposed to Chronic Unpredictable Mild Stress is Mediated Through Antioxidative, Anti-Inflammatory, Anti-Apoptotic, and Neuromodulatory Activities

    DOI: 10.2147/IJN.S323436

    PMID: 35002238

    		 Sample: Hippocampal Tissue
  2. SCIENCE OF THE TOTAL ENVIRONMENT (2020) IF: 6.551
    Protocatechuic acid mitigates cadmium-induced neurotoxicity in rats: Role of oxidative stress, inflammation and apoptosis

    DOI: 10.1016/j.scitotenv.2020.137969
    Sample: cortical
  3. LIFE SCIENCES (2022) IF: 6.78
    Irbesartan reprofiling for the amelioration of ethanol-induced gastric mucosal injury in rats: Role of inflammation, apoptosis, and autophagy

    DOI: 10.1016/j.lfs.2022.120939

    PMID: 36115582

    		 Sample: gastric tissue
  4. LIFE SCIENCES (2021) IF: 5.037
    The efficacy of bone marrow-derived mesenchymal stem cells and/or erythropoietin in ameliorating kidney damage in gamma irradiated rats: Role of non-hematopoietic erythropoietin anti-apoptotic signaling

    DOI: 10.1016/j.lfs.2021.119388

    PMID: 33774028

    		 Sample: Tissue homogenate
  5. PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY (2021) IF: 5.067
    Nano-ivabradine averts behavioral anomalies in Huntington's disease rat model via modulating Rhes/m-tor pathway

    DOI: 10.1016/j.pnpbp.2021.110368

    PMID: 34087391

    		 Sample: serum
  6. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2023) IF: 5.19
    Betulinic acid protects against cardiotoxicity of the organophosphorus pesticide chlorpyrifos by suppressing oxidative stress, inflammation, and apoptosis in rats

    DOI: 10.1007/s11356-023-25917-6
    Sample: heart
  7. Pharmaceuticals (2022) IF: 5.215
    Lycopene Modulates Oxidative Stress and Inflammation in Hypercholesterolemic Rats

    DOI: 10.3390/ph15111420
    Sample: Cardiac,Renal
  8. INTERNATIONAL IMMUNOPHARMACOLOGY (2023) IF: 5.714
    Donepezil halts acetic acid-induced experimental colitis in rats and its associated cognitive impairment through regulating inflammatory/oxidative/apoptotic cascades: An add-on to its anti-dementia activity

    DOI: 10.1016/j.intimp.2023.109841
    Sample: Colon
  9. Genes (2022) IF: 4.141
    Nrf2/HO-1 Signaling Stimulation through Acetyl-11-Keto-Beta-Boswellic Acid (AKBA) Provides Neuroprotection in Ethidium Bromide-Induced Experimental Model of Multiple Sclerosis

    DOI: 10.3390/genes13081324

    PMID: 35893061

    		 Sample: brain
  10. TOXICOLOGY (2021) IF: 4.221
    3-Indolepropionic acid upturned male reproductive function by reducing oxido-inflammatory responses and apoptosis along the hypothalamic-pituitary-gonadal axis of adult rats exposed to chlorpyrifos

    DOI: 10.1016/j.tox.2021.152996

    PMID: 34678318

    		 Sample: testes,epididymis,hypothalamus
  11. Frontiers in Pharmacology (2020) IF: 4.225
    Licorice Ameliorates Cisplatin-Induced Hepatotoxicity Through Antiapoptosis, Antioxidative Stress, Anti-Inflammation, and Acceleration of Metabolism

    DOI: 10.3389/fphar.2020.563750

    PMID: 33240085

    		 Sample: Tissue homogenate
  12. NEUROTOXICOLOGY (2018) IF: 3.076
    Antiapoptotic effect of taurine against NMDA-induced retinal excitotoxicity in rats

    DOI: 10.1016/j.neuro.2018.10.009

    PMID: 30385388

    		 Sample: Tissue homogenate
  13. NEUROTOXICITY RESEARCH (2016) IF: 3.14
    Neuroprotective Effect of Magnesium Acetyltaurate Against NMDA-Induced Excitotoxicity in Rat Retina

    DOI: 10.1007/s12640-016-9658-9
    Sample: Tissue homogenate
  14. ACS Omega (2022) IF: 3.512
    Sarcococca saligna Hydroalcoholic Extract Ameliorates Arthritis in Complete Freund’s Adjuvant-Induced Arthritic Rats via Modulation of Inflammatory Biomarkers and Suppression of Oxidative Stress Markers

    DOI: 10.1021/acsomega.2c00619
    Sample: serum
  15. METABOLIC BRAIN DISEASE (2021) IF: 3.584
    Guggulsterone ameliorates ethidium bromide-induced experimental model of multiple sclerosis via restoration of behavioral, molecular, neurochemical and morphological alterations in rat brain

    DOI: 10.1007/s11011-021-00691-x

    PMID: 33635478

    		 Sample: Tissue homogenate(brain)
  16. LIFE SCIENCES (2020) IF: 3.647
    Beneficial effects of ferulic acid alone and in combination with insulin in streptozotocin induced diabetic neuropathy in Sprague Dawley rats

    DOI: 10.1016/j.lfs.2020.117856
    Sample: Tissue homogenate
  17. JOURNAL OF FOOD BIOCHEMISTRY (2022) IF: 3.654
    Twelve weeks of treadmill exercise training with green tea extract reduces myocardial oxidative stress and alleviates cardiomyocyte apoptosis in aging rat: The emerging role of BNIP3 and HIF-1α/IGFBP3 pathway

    DOI: 10.1111/jfbc.14397

    PMID: 36069470

    		 Sample: heart tissue
  18. METABOLIC BRAIN DISEASE (2022) IF: 3.655
    Sarcococca saligna ameliorated D-galactose induced neurodegeneration through repression of neurodegenerative and oxidative stress biomarkers

    DOI: 10.1007/s11011-022-01046-w

    PMID: 35881299

    		 Sample: Brain tissue
  19. NEUROCHEMICAL RESEARCH (2022) IF: 3.996
    Tetramethylpyrazine Attenuates Cognitive Impairment Via Suppressing Oxidative Stress, Neuroinflammation, and Apoptosis in Type 2 Diabetic Rats

    DOI: 10.1007/s11064-022-03640-x

    PMID: 35665448

    		 Sample: hippocampal tissue
  20. ANDROLOGIA (2021) IF: 2.775
    Decrease in reproductive dysfunction using aflatoxin B1 exposure: a treatment with 3-indolepropionic acid in albino Wistar rat

    DOI: 10.1111/and.14248

    PMID: 34541692

    		 Sample: testicular,epididymal,hypothalamic
  21. NEUROSCIENCE RESEARCH (2023) IF: 2.904
    Neuroprotection by trans-resveratrol in rats with N-methyl-D-aspartate (NMDA)–induced retinal injury: insights into the role of adenosine A1 receptors

    DOI: 10.1016/j.neures.2023.02.004
    Sample: retinal
  22. Molecular Medicine Reports (2022) IF: 2.952
    Propofol postconditioning alleviates diabetic myocardial ischemia‑reperfusion injury via the miR‑200c‑3p/AdipoR2/STAT3 signaling pathway

    DOI: 10.3892/mmr.2022.12653
    Sample: serum
  23. ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS (2020) IF: 1.28
    Involvement of redox status and the nuclear-related factor 2 in protecting against cadmium-induced renal injury with Sana Makki (Cassia senna L.) pre-treatment in male rats

    DOI: 10.1590/0001-3765202020191237
    Sample: Serum,Tissue homogenate
  24. Iranian Journal of Basic Medical Sciences (2018) IF: 1.514
    Sleep deprivation disrupts striatal anti-apoptotic responses in 6-hydroxy dopamine-lesioned parkinsonian rats

    DOI: 10.22038/ijbms.2018.28546.6919

    PMID: 30627374

    		 Sample: Tissue homogenate
  25. Experimental and Therapeutic Medicine (2020) IF: 1.785
    miR‑9 inhibition of neuronal apoptosis and expression levels of apoptosis genes Bcl‑2 and Bax in depression model rats through Notch pathway

    DOI: 10.3892/etm.2019.8228
    Sample: serum
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