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Rat BDNF(Brain Derived Neurotrophic Factor) ELISA Kit

Citations(53)Uniprot : P23363

  • Cat.No.:E-EL-R1235

  • Reactivity: Rat
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  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat BDNF in samples. No significant cross-reactivity or interference between Rat BDNF and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat BDNF concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat BDNF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BDNF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BDNF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat BDNF. You can calculate the concentration of Rat BDNF in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat BDNF were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat BDNF were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 107.40 179.20 874.50 103.20 162.30 829.50
Standard deviation 7.00 8.40 35.00 6.60 8.00 40.60
CV (%) 6.52 4.69 4.00 6.40 4.93 4.89

Recovery

The recovery of Rat BDNF spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 94-108 99
EDTA plasma (n=8) 92-107 99
Cell culture media (n=8) 83-98 90

Linearity

Samples were spiked with high concentrations of Rat BDNF and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P23363
SynonymsANON2, BULN2
Research AreaCancer, Cardiovascular, Metabolism, Neuroscience

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. ADVANCED FUNCTIONAL MATERIALS (2018) IF: 13.325
    MRI‐Visible siRNA Nanomedicine Directing Neuronal Differentiation of Neural Stem Cells in Stroke

    DOI: 10.1002/adfm.201706769
    Sample: neural stem cells (NSCs)
  2. Advanced Healthcare Materials (2022) IF: 11.092
    Engineered Living Oriented Electrospun Fibers Regulate Stem Cell Para-Secretion and Differentiation to Promote Spinal Cord Repair

    DOI: 10.1002/adhm.202202785
    Sample: BMSCs
  3. Materials Today Advances (2023) IF: 9.9179
    Bone marrow mesenchymal stem cells loaded into hydrogel/nanofiber composite scaffolds ameliorate ischemic brain injury

    DOI: 10.1016/j.mtadv.2023.100349
    Sample: bone marrow mesenchymal stem cells (BMSCs)
  4. BIOMEDICINE & PHARMACOTHERAPY (2023) IF: 7.419
    Vildagliptin restores cognitive function and mitigates hippocampal neuronal apoptosis in cisplatin-induced chemo-brain: Imperative roles of AMPK/Akt/CREB/ BDNF signaling cascades

    DOI: 10.1016/j.biopha.2023.114238
    Sample: hippocampal tissue
  5. Neurotherapeutics (2021) IF: 7.62
    Crosstalk Among NLRP3 Inflammasome, ETBR Signaling, and miRNAs in Stress-Induced Depression-Like Behavior: a Modulatory Role for SGLT2 Inhibitors

    DOI: 10.1007/s13311-021-01140-4

    PMID: 34664178

    		 Sample: hippocampal
  6. Antioxidants (2022) IF: 7.675
    Comparable Benefits of Stingless Bee Honey and Caffeic Acid in Mitigating the Negative Effects of Metabolic Syndrome on the Brain

    DOI: 10.3390/antiox11112154

    PMID: 36358526

    		 Sample: brain tissue
  7. Frontiers in Cellular and Infection Microbiology (2022) IF: 6.073
    Changes in intestinal microflora and its metabolites underlie the cognitive impairment in preterm rats

    DOI: 10.3389/fcimb.2022.945851

    PMID: 36061856

    		 Sample: serum
  8. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023) IF: 6.208
    Dexmedetomidine Attenuates Methotrexate-Induced Neurotoxicity and Memory Deficits in Rats through Improving Hippocampal Neurogenesis: The Role of miR-15a/ROCK-1/ERK1/2/CREB/BDNF Pathway Modulation

    DOI: 10.3390/ijms24010766
    Sample: Hippocampal Tissue
  9. LIFE SCIENCES (2021) IF: 5.037
    Berberine ameliorates doxorubicin-induced cognitive impairment (chemobrain) in rats

    DOI: 10.1016/j.lfs.2021.119078

    PMID: 33460662

    		 Sample: Tissue homogenate(Brain)
  10. MOLECULAR NEUROBIOLOGY (2018) IF: 5.076
    Neuroprotective Effects of Filgrastim in Rotenone-Induced Parkinson’s Disease in Rats: Insights into its Anti-Inflammatory, Neurotrophic, and Antiapoptotic Effects

    DOI: 10.1007/s12035-017-0855-1
    Sample: Midbrain,striatal
  11. Food & Function (2021) IF: 5.396
    Neuroprotective effects of macamide from maca (Lepidium meyenii Walp.) on corticosterone-induced hippocampal impairments through anti-inflammatory, neurotrophic, and synaptic protection properties

    DOI: 10.1039/D1FO01720A
    Sample: hippocampu
  12. MOLECULAR NEUROBIOLOGY (2022) IF: 5.682
    Adenosine A2A Receptor Blockade Ameliorates Mania Like Symptoms in Rats: Signaling to PKC-α and Akt/GSK-3β/β-Catenin

    DOI: 10.1007/s12035-022-02977-2

    PMID: 35943710

    		 Sample: prefrontal cortex (PFC)
  13. ACS Chemical Neuroscience (2022) IF: 5.78
    Granulocyte Colony-Stimulating Factor Improved Core Symptoms of Autism Spectrum Disorder via Modulating Glutamatergic Receptors in the Prefrontal Cortex and Hippocampus of Rat Brains

    DOI: 10.1021/acschemneuro.2c00270

    PMID: 36166499

    		 Sample: hippocampus tissue,pre frontal cortex
  14. Frontiers in Pharmacology (2021) IF: 5.811
    Rivaroxaban Modulates TLR4/Myd88/NF-Kβ Signaling Pathway in a Dose-Dependent Manner With Suppression of Oxidative Stress and Inflammation in an Experimental Model of Depression

    DOI: 10.3389/fphar.2021.715354

    PMID: 34630092

    		 Sample: hippocampal tissue
  15. Frontiers in Pharmacology (2021) IF: 5.811
    l-Borneol Exerted the Neuroprotective Effect by Promoting Angiogenesis Coupled With Neurogenesis via Ang1-VEGF-BDNF Pathway

    DOI: 10.3389/fphar.2021.641894

    PMID: 33746762

    		 Sample: Serum
  16. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021) IF: 5.924
    Metabolic Disturbances in Rat Sublines with Constitutionally Altered Serotonin Homeostasis

    DOI: 10.3390/ijms22105400

    PMID: 34065591

    		 Sample: Tissue homogenate
  17. BRAIN RESEARCH BULLETIN (2021) IF: 4.079
    Role of protein tyrosine phosphatase 1B inhibitor in central insulin resistance and associated cognitive deficits

    DOI: 10.1016/j.brainresbull.2021.02.026

    PMID: 33684458

    		 Sample: Tissue homogenate
  18. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2021) IF: 4.223
    Melissa officinalis L. ameliorates oxidative stress and inflammation and upregulates Nrf2/HO-1 signaling in the hippocampus of pilocarpine-induced rats

    DOI: 10.1007/s11356-021-15825-y

    PMID: 34363578

    		 Sample: hippocampus
  19. COLLOIDS AND SURFACES B-BIOINTERFACES (2020) IF: 4.389
    Solid lipid nanoparticles-mediated enhanced antidepressant activity of duloxetine in lipopolysaccharide-induced depressive model

    DOI: 10.1016/j.colsurfb.2020.111209

    PMID: 32599505

    		 Sample: Plasma,Tissue homogenate
  20. INFLAMMOPHARMACOLOGY (2021) IF: 4.473
    Neuroprotective effects of roflumilast against quinolinic acid-induced rat model of Huntington’s disease through inhibition of NF-κB mediated neuroinflammatory markers and activation of cAMP/CREB/BDNF signaling pathway

    DOI: 10.1007/s10787-020-00787-3
    Sample: Tissue homogenate(brain)
  21. NEUROSCIENCE LETTERS (2022) IF: 3.046
    Differential effects of voluntary and forced exercise trainings on spatial learning ability and hippocampal biomarkers in aged female rats

    DOI: 10.1016/j.neulet.2022.136499

    PMID: 35121056

    		 Sample: hippocampus
  22. NEUROTOXICOLOGY (2018) IF: 3.076
    Antiapoptotic effect of taurine against NMDA-induced retinal excitotoxicity in rats

    DOI: 10.1016/j.neuro.2018.10.009

    PMID: 30385388

    		 Sample: Tissue homogenate
  23. JOURNAL OF ETHNOPHARMACOLOGY (2018) IF: 3.115
    Validation of ethnopharmacology of ayurvedic sarasvata ghrita and comparative evaluation of its neuroprotective effect with modern alcoholic and lipid based extracts in β-amyloid induced memory impairment

    DOI: 10.1016/j.jep.2018.02.032
    Sample: Plasma
  24. BEHAVIOURAL BRAIN RESEARCH (2018) IF: 3.173
    Immediate and long-term antidepressive-like effects of pre-pubertal escitalopram and omega-3 supplementation combination in young adult stress-sensitive rats

    DOI: 10.1016/j.bbr.2018.05.021
    Sample: Tissue homogenate
  25. ACTA NEUROPSYCHIATRICA (2021) IF: 3.403
    The long-term bio-behavioural effects of juvenile sildenafil treatment in Sprague-Dawley versus flinders sensitive line rats

    DOI: 10.1017/neu.2021.4

    PMID: 33593460

    		 Sample: Tissue homogenate(hippocampal)
  26. Biomed Research International (2022) IF: 3.411
    Affective Antidepressant, Cytotoxic Activities, and Characterization of Phyto-Assisted Zinc Oxide Nanoparticles Synthesized Using Sanvitalia procumbens Aqueous Extract

    DOI: 10.1155/2022/1621372

    PMID: 35757480

    		 Sample: plasma,brain
  27. LIFE SCIENCES (2019) IF: 3.448
    Effects of noopept on cognitive functions and pubertal process in rats with diabetes

    DOI: 10.1016/j.lfs.2019.116698

    PMID: 31356906

    		 Sample: Serum,Tissue homogenate
  28. SAUDI PHARMACEUTICAL JOURNAL (2019) IF: 3.643
    Propolis ameliorates cerebral injury in focal cerebral ischemia/reperfusion (I/R) rat model via upregulation of TGF-β1

    DOI: 10.1016/j.jsps.2019.11.013

    PMID: 31920438

    		 Sample: Tissue homogenate
  29. LIFE SCIENCES (2020) IF: 3.647
    Glutamatergic pathway in depressive-like behavior associated with pentylenetetrazole rat model of epilepsy with history of prolonged febrile seizures

    DOI: 10.1016/j.lfs.2020.117692
    Sample: Tissue homogenate
  30. CHEMICO-BIOLOGICAL INTERACTIONS (2020) IF: 3.723
    Anti-depressant effect of cerebrolysin in reserpine-induced depression in rats: Behavioral, biochemical, molecular and immunohistochemical evidence

    DOI: 10.1016/j.cbi.2020.109329

    PMID: 33279466

    		 Sample: Cortical,hippocampal
  31. JOURNAL OF PHARMACY AND PHARMACOLOGY (2021) IF: 3.765
    New insights into the effects of vinpocetine against neurobehavioral comorbidities in a rat model of temporal lobe epilepsy via the downregulation of the hippocampal PI3K/mTOR signalling pathway

    DOI: 10.1093/jpp/rgab011
    Sample: hippocampi tissue
  32. REJUVENATION RESEARCH (2019) IF: 3.811
    The Effects of Light and Moderate Intensity Exercise on the Femoral Bone and Cerebellum of d-Galactose-Exposed Rats

    DOI: 10.1089/rej.2018.2050
    Sample: Serum,Tissue homogenate
  33. NEUROCHEMISTRY INTERNATIONAL (2019) IF: 3.994
    Lercanidipine boosts the efficacy of mesenchymal stem cell therapy in 3-NP-induced Huntington's disease model rats via modulation of the calcium/calcineurin/NFATc4 and Wnt/β-catenin signalling pathways

    DOI: 10.1016/j.neuint.2019.104548
    Sample: brain
  34. NEUROCHEMICAL RESEARCH (2021) IF: 3.996
    Metabolomic Profiling and Neuroprotective Effects of Purslane Seeds Extract Against Acrylamide Toxicity in Rat’s Brain

    DOI: 10.1007/s11064-020-03209-6
    Sample: Tissue homogenate(Brain)
  35. Iranian Journal of Basic Medical Sciences (2020) IF: 2.146
    Effect of doxycycline and meloxicam on cytokines, brain-derived neurotrophic factor, matrix metalloproteinase-3, tissue inhibitor of metalloproteinase-3 and cyclooxygenase-2 in brain

    DOI: 10.22038/ijbms.2020.45193.10527

    PMID: 33149866

    		 Sample: Tissue homogenate
  36. PHYSIOLOGY & BEHAVIOR (2017) IF: 2.341
    High intensity interval training modulates hippocampal oxidative stress, BDNF and inflammatory mediators in rats

    DOI: 10.1016/j.physbeh.2017.10.027
    Sample: Tissue homogenate
  37. Neural Regeneration Research (2019) IF: 2.472
    Proprotein convertase 1/3-mediated down-regulation of brain-derived neurotrophic factor in cortical neurons induced by oxygen-glucose deprivation

    DOI: 10.4103/1673-5374.270314

    PMID: 31823886

    		 Sample: Cell culture supernatant
  38. NEUROLOGICAL RESEARCH (2023) IF: 2.529
    The effect of the calcium channel blocker nimodipine on hippocampal BDNF/Ach levels in rats with experimental cognitive impairment

    DOI: 10.1080/01616412.2022.2164452
    Sample: Hippocampal,prefrontal cortex
  39. Iranian Journal of Basic Medical Sciences (2021) IF: 2.699
    Adrenomedullin increases cAMP accumulation and BDNF expression in rat DRG and spinal motor neurons

    DOI: 10.22038/ijbms.2021.54796.12289

    PMID: 34712429

    		 Sample: brain
  40. JOURNAL OF FOOD BIOCHEMISTRY (2021) IF: 2.72
    6-Gingerol, an active constituent of ginger, attenuates lipopolysaccharide-induced oxidation, inflammation, cognitive deficits, neuroplasticity, and amyloidogenesis in rat

    DOI: 10.1111/jfbc.13660

    PMID: 33624846

    		 Sample: brain
  41. JOURNAL OF NEUROSCIENCE RESEARCH (2014) IF: 2.729
    Furin mediates brain‐derived neurotrophic factor upregulation in cultured rat astrocytes exposed to oxygen–glucose deprivation

    DOI: 10.1002/jnr.23455
    Sample: Cell culture supernatant
  42. FUNDAMENTAL & CLINICAL PHARMACOLOGY (2021) IF: 2.748
    Everolimus: A potential therapeutic agent targeting PI3K/Akt pathway in brain insulin system dysfunction and associated neurobehavioral deficits

    DOI: 10.1111/fcp.12677

    PMID: 33783880

    		 Sample: Tissue homogenate(hippocampus)
  43. BIOMEDICINE & PHARMACOTHERAPY (2017) IF: 2.759
    Neuroprotective effect of Indian propolis in β-amyloid induced memory deficit: Impact on behavioral and biochemical parameters in rats

    DOI: 10.1016/j.biopha.2017.06.072
    Sample: Plasma
  44. BEHAVIOURAL BRAIN RESEARCH (2019) IF: 2.77
    Voluntary, involuntary and forced exercises almost equally reverse behavioral impairment by regulating hippocampal neurotrophic factors and oxidative stress in experimental Alzheimer’s disease model

    DOI: 10.1016/j.bbr.2019.02.030
    Sample: Tissue homogenate
  45. PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR (2019) IF: 2.773
    Co-treatment of piracetam with risperidone rescued extinction deficits in experimental paradigms of post-traumatic stress disorder by restoring the physiological alterations in cortex and hippocampus

    DOI: 10.1016/j.pbb.2019.172763
    Sample: plasma
  46. ANDROLOGIA (2021) IF: 2.775
    Expression of brain-derived neurotrophic factor in rapid ejaculator rats: A further study

    DOI: 10.1111/and.14134

    PMID: 34101873

    		 Sample: blood,brain,spinal cord
  47. BRAIN RESEARCH (2019) IF: 2.929
    Effect of enriched environment and predictable chronic stress on spatial memory in adolescent rats: Predominant expression of BDNF, nNOS, and interestingly malondialdehyde in the right hippocampus

    DOI: 10.1016/j.brainres.2019.146326
    Sample: hippocampus
  48. Iranian Journal of Basic Medical Sciences (2018) IF: 1.514
    Sleep deprivation disrupts striatal anti-apoptotic responses in 6-hydroxy dopamine-lesioned parkinsonian rats

    DOI: 10.22038/ijbms.2018.28546.6919

    PMID: 30627374

    		 Sample: Tissue homogenate
  49. Experimental and Therapeutic Medicine (2020) IF: 1.785
    Effects of Vortioxetine on depression model rats and expression of BDNF and Trk B in hippocampus

    DOI: 10.3892/etm.2020.9026

    PMID: 32765787

    		 Sample: Tissue homogenate
  50. INTERNATIONAL JOURNAL OF NEUROSCIENCE (2018) IF: 1.848
    Time- and dose-related effects of amyloid beta1-40 on retina and optic nerve morphology in rats

    DOI: 10.1080/00207454.2018.1446953
    Sample: Tissue homogenate
  51. Advances in Traditional Medicine (2022)
    Nootropic effect of Syzygium polyanthum (Wight) Walp leaf extract in chronic cerebral hypoperfusion rat model via cholinergic restoration: a potential therapeutic agent for dementia

    DOI: 10.1007/s13596-022-00653-3
    Sample: brain
  52. International Journal of Clinical and Experimental Pathology (2019) IF: 0.205
    The protective role of Schwann cells in bladder smooth muscle cell fibrosis

    PMID: 31933768

    		 Sample: bladder,BSMCs cells
  53. INDIAN JOURNAL OF PHARMACOLOGY (2020) IF: 0.888
    Mangiferin ameliorates intracerebroventricular-quinolinic acid-induced cognitive deficits, oxidative stress, and neuroinflammation in Wistar rats

    DOI: 10.4103/ijp.IJP_699_19
    Sample: Tissue homogenate
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Reviews/Q&A

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Q

a***aSubmitted [ Mar 17 2023 ]

Asked: can i use rat brain homogenate for performing this assay ?

Reply

A

adminSubmitted [ Mar 17 2023 ]

Answered: E-EL-R1235 Rat BDNF(Brain Derived Neurotrophic Factor) ELISA Kit The kit can be used for rat brain homogenates.

Q

a***aSubmitted [ Mar 17 2023 ]

Asked: Does tissue homogenate can be used ?

Reply

A

adminSubmitted [ Mar 17 2023 ]

Answered: E-EL-R1235 Rat BDNF(Brain Derived Neurotrophic Factor) ELISA Kit The kit can be used for rat brain homogenates.

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