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Rat BDNF(Brain Derived Neurotrophic Factor) ELISA Kit

  • Cat.No.:E-EL-R1235

  • Reactivity: Rat

To Purchase E-EL-R1235

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat BDNF in samples. No significant cross-reactivity or interference between Rat BDNF and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat BDNF concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat BDNF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BDNF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BDNF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat BDNF. You can calculate the concentration of Rat BDNF in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat BDNF were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat BDNF were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 107.40 179.20 874.50 103.20 162.30 829.50
Standard deviation 7.00 8.40 35.00 6.60 8.00 40.60
CV (%) 6.52 4.69 4.00 6.40 4.93 4.89


The recovery of Rat BDNF spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 94-108 99
EDTA plasma (n=8) 92-107 99
Cell culture media (n=8) 83-98 90


Samples were spiked with high concentrations of Rat BDNF and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P23363
SynonymsANON2, BULN2
Research AreaCancer, Cardiovascular, Metabolism, Neuroscience

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Colloids and Surfaces B: Biointerfaces (2020) IF: 4.389
    Solid lipid nanoparticles-mediated enhanced antidepressant activity of duloxetine in lipopolysaccharide-induced depressive model

    DOI: 10.1016/j.colsurfb.2020.111209

    PMID: 32599505

    Sample: Plasma,Tissue homogenate
  2. Neural Regeneration Research (2020) IF: 3.171
    Proprotein convertase 1/3-mediated down-regulation of brain-derived neurotrophic factor in cortical neurons induced by oxygen-glucose deprivation

    DOI: 10.4103/1673-5374.270314

    PMID: 31823886

    Sample: Cell culture supernatant
  3. Life sciences (2019) IF: 3.647
    Effects of noopept on cognitive functions and pubertal process in rats with diabetes

    DOI: 10.1016/j.lfs.2019.116698

    PMID: 31356906

    Sample: Serum,Tissue homogenate
  4. Neurotoxicology (2019) IF: 3.076
    Antiapoptotic effect of taurine against NMDA-induced retinal excitotoxicity in rats

    DOI: 10.1016/j.neuro.2018.10.009

    PMID: 30385388

    Sample: Tissue homogenate
  5. Behavioural brain research (2019) IF: 3.173
    Voluntary, involuntary and forced exercises almost equally reverse behavioral impairment by regulating hippocampal neurotrophic factors and oxidative stress in experimental Alzheimer's disease model

    DOI: 10.1016/j.bbr.2019.02.030

    PMID: 30790584

    Sample: Tissue homogenate
  6. Behavioural brain research (2018) IF: 3.173
    Immediate and long-term antidepressive-like effects of pre-pubertal escitalopram and omega-3 supplementation combination in young adult stress-sensitive rats.

    DOI: 10.1016/j.bbr.2018.05.021

    PMID: 29807070

    Sample: Tissue homogenate
  7. Rejuvenation research (2018) IF: 3.22

    DOI: 10.1089/rej.2018.2050

    PMID: 29962322

    Sample: Serum, Tissue homogenate
  8. Saudi Pharmaceutical Journal (2020) IF: 2.879
    Propolis ameliorates cerebral injury in focal cerebral ischemia/reperfusion (I/R) rat model via upregulation of TGF-β1

    DOI: 10.1016/j.jsps.2019.11.013

    PMID: 31920438

    Sample: Tissue homogenate
  9. Journal of Ethnopharmacology (2018) IF: 2.981
    Validation Of Ethnopharmacology Of Ayurvedic Sarasvata Ghrita And Comparative Evaluation Of Its Neuroprotective Effect With Modern Alcoholic And Lipid Based Extracts In Β-Amyloid Induced Memory Impairment.

    DOI: 10.1016/j.jep.2018.02.032

    PMID: 29501676

  10. Journal of Neuroscience Research (2015) IF: 2.481
    urin Mediates Brain-Derived Neurotrophic Factor Upregulation In Cultured Rat Astrocytes Exposed To Oxygen-Glucose Deprivation

    DOI: 10.1002/jnr.23455

    PMID: 25074577

    Sample: Cell culture supernatant
  11. Physiology & Behavior (2017) IF: 2.341
    High Intensity Interval Training Modulates Hippocampal Oxidative Stress, BDNF and Inflammatory Mediators In Rats.

    DOI: 10.1016/j.physbeh.2017.10.027

    PMID: 29111230

    Sample: Tissue homogenate
  12. Biomedicine & Pharmacotherapy (2017) IF: 2.759
    Neuroprotective Effect Of Indian Propolis In β-Amyloid Induced Memory Deficit: Impact On Behavioral And Biochemical Parameters In Rats.

    DOI: 10.1016/j.biopha.2017.06.072

    PMID: 28686968

    Sample: Plasma
  13. Journal of Neuroscience Research (2015) IF: 2.481
    Furin Mediates Brain-Derived Neurotrophic Factor Upregulation In Cultured Rat Astrocytes Exposed To Oxygen-Glucose Deprivation.

    DOI: 10.1002/jnr.23455

    PMID: 25074577

    Sample: Cell culture supernatant
  14. Experimental and Therapeutic Medicine (2020) IF: 1.785
    Effects of Vortioxetine on depression model rats and expression of BDNF and Trk B in hippocampus

    DOI: 10.3892/etm.2020.9026

    PMID: 32765787

    Sample: Tissue homogenate
  15. The Journal of Pure and Applied Chemistry Research (2018) IF: 1.349
    Potential of Anthocyanin From Purple Sweet Potato (Ipomoea batatas) To Increase BDNF Level and VEGF Expression in The Cerebellum of Ischemic Stroke Rats

    DOI: 10.21776/ub.jpacr.2018.007.01.363

    Sample: Tissue homogenate
  16. Iranian journal of basic medical sciences (2018) IF: 1.514
    Sleep deprivation disrupts striatal anti-apoptotic responses in 6-hydroxy dopamine-lesioned parkinsonian rats

    DOI: 10.22038/ijbms.2018.28546.6919

    PMID: 30627374

    Sample: Tissue homogenate
  17. International Journal of Neuroscience (2018) IF: 1.75
    Time-And Dose-Related Effects Of Amyloid BETA 1-40 On Retina And Optic Nerve Morphology In Rats.

    DOI: 10.1080/00207454.2018.1446953

    PMID: 29488424

  18. International Journal of Clinical and Experimental Pathology (2019) IF: 0.252
    The protective role of Schwann cells in bladder smooth muscle cell fibrosis

    PMID: 31933768

    Sample: Cell culture supernatant,Tissue homogenate
  19. Vojnosanitetski Pregled (2018) IF: 0.405
    Chronic physical exercise increases a neurogenesis marker within hippocampus

    DOI: 10.13181/mji.v27i2.1685

    Sample: Tissue homogenate
  20. Journal of SCRTE (2018)
    Brain Derived Neurotrophic Factor Levels in Aged Rats Post-Systemic Human Mesenchymal Stem Cell Administration

    DOI: 10.20473/jscrte.v2i2.11895

    Sample: Tissue homogenate
  21. Medical Journal of Indonesia (2018) IF: /
    Chronic physical exercise increases a neurogenesis marker within hippocampus.

    DOI: 10.13181/mji.v27i2.1685

    Sample: Tissue homogenate
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