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Rat BMP-2(Bone Morphogenetic Protein 2) ELISA Kit

  • Cat.No.:E-EL-R0002

  • Reactivity: Rat

To Purchase E-EL-R0002

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 62.50-4000 pg/mL
Sensitivity 37.50 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat BMP-2 in samples. No significant cross-reactivity or interference between Rat BMP-2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat BMP-2 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat BMP-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BMP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BMP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat BMP-2. You can calculate the concentration of Rat BMP-2 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat BMP-2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat BMP-2 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 182.20 368.60 1622.50 178.60 372.20 1493.30
Standard deviation 11.10 15.50 87.60 9.50 21.20 76.20
CV (%) 6.09 4.21 5.40 5.32 5.70 5.10


The recovery of Rat BMP-2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-104 99
EDTA plasma (n=8) 90-104 97
Cell culture media (n=8) 87-97 92


Samples were spiked with high concentrations of Rat BMP-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P49001
SynonymsBMP2A, BMP-2A, BDA2
Research AreaCancer, Cardiovascular, Signal transduction, Stem cells

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. BIOMATERIALS (2022) IF: 15.304
    Immunology and bioinformatics analysis of injectable organic/inorganic microfluidic microspheres for promoting bone repair

    DOI: 10.1016/j.biomaterials.2022.121685

    PMID: 35953327

    Sample: BMSCs
  2. Advanced Healthcare Materials (2022) IF: 11.092
    Spatiotemporal regulation of the bone immune microenvironment via dam-like biphasic bionic periosteum for bone regeneration

    DOI: 10.1002/adhm.202201661

    PMID: 36189833

    Sample: BMSCs
  3. Bio-Design and Manufacturing (2022) IF: 6.302
    Injectable bioactive polymethyl methacrylate–hydrogel hybrid bone cement loaded with BMP-2 to improve osteogenesis for percutaneous vertebroplasty and kyphoplasty

    DOI: 10.1007/s42242-021-00172-1

    Sample: bone marrow mesenchymal stem cell (BMSCs)
  4. Biomaterials Science (2019) IF: 6.843
    ONO-1301 loaded nanocomposite scaffolds modulate cAMP mediated signaling and induce new bone formation in critical sized bone defect

    DOI: 10.1039/C9BM01352K

    Sample: Mesenchymal stem cell
  5. CHEMOSPHERE (2015) IF: 3.34
    Effects of nitrate on metamorphosis, thyroid and iodothyronine deiodinases expression in Bufo gargarizans larvae

    DOI: 10.1016/j.chemosphere.2015.07.037

    Sample: Tissue homogenate
    Tibolone, alendronate, and simvastatin enhance implant osseointegration in a preclinical in vivo model

    DOI: 10.1111/clr.13602

    Sample: blood
    Hyaluronic Acid Modified Risedronate and Teriparatide Co-loaded Nanocarriers for Improved Osteogenic Differentiation of Osteoblasts for the Treatment of Osteoporosis

    DOI: 10.2174/1381612825666190801140703

    Sample: MC3T3-E1 cells
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