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Rat BNP(Brain Natriuretic Peptide) ELISA Kit

Cat.No.:E-EL-R0126
Reactivity: Rat

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Product Details

Properties

Assay type	Competitive-ELISA
Format	96T/48T
Assay time	2.0h
Detection range	31.25-2000 pg/mL
Sensitivity	18.75 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 50μL
Specificity	This kit recognizes BNP in samples. No significant cross-reactivity or interference between BNP and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of BNP concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with BNP. During the reaction, BNP in the sample or standard competes with a fixed amount of BNP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to BNP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of BNP in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8℃
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level BNP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level BNP were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	96.20	313.10	672.80	97.20	338.80	634.50
Standard deviation	5.90	14.70	32.30	6.60	17.30	29.20
CV (%)	6.13	4.69	4.80	6.79	5.11	4.60

Recovery

The recovery of BNP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	93-107	101
EDTA plasma(n=8)	84-97	91
Cell culture media(n=8)	86-98	92

Linearity

Samples were spiked with high concentrations of Rat BNP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	NPPB, GC-B, B-Type Natriuretic Peptide, Natriuretic Peptide Precursor B
Research Area	Cancer, Cardiovascular, Neuroscience

Assay Procedures

	1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C
	2. Aspirate and wash the plate for 3 times
	3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	5. Add 50μL Stop Solution
	6. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

Cellular Physiology and Biochemistry (2018) IF: 5.104

Renal Denervation Effects on Myocardial Fibrosis and Ventricular Arrhythmias in Rats with Ischemic Cardiomyopathy.

DOI: 10.1159/000489653
PMID: 29742489

Frontiers in Pharmacology (2020) IF: 4.225

Perindopril Improves Cardiac Function by Enhancing the Expression of SIRT3 and PGC-1α in a Rat Model of Isoproterenol-Induced Cardiomyopathy

DOI: 10.3389/fphar.2020.00094
PMID: 32153406	Sample: Serum

Biomedicine & Pharmacotherapy (2019) IF: 3.457

The effects of Radix Angelica Sinensis and Radix Hedysari ultrafiltration extract on X-irradiation-induced myocardial fibrosis in rats

DOI: 10.1016/j.biopha.2019.01.057
PMID: 30780109	Sample: Serum

Biochemical and biophysical research communications (2019) IF: 2.985

Therapeutic effects of erythropoietin expressed in mesenchymal stem cells for dilated cardiomyopathy in rat

DOI: 10.1016/j.bbrc.2019.07.053
PMID: 31400858	Sample: Serum,Tissue homogenate

Acta biochimica et biophysica Sinica (2018) IF: 2.224

AdipoRon prevents l-thyroxine or isoproterenol-induced cardiac hypertrophy through regulating the AMPK-related pathway

DOI: 10.1093/abbs/gmy152
PMID: 30566571	Sample: Serum

Experimental and therapeutic medicine (2018) IF: 1.41

Effect of parathyroid hormone on cardiac function in rats with cardiomyopathy

DOI: 10.3892/etm.2018.6528
PMID: 30214507	Sample: Serum

Biological and Pharmaceutical Bulletin (2018) IF: 1.54

Qiliqiangxin Protects against Renal Injury in Rat with Cardiorenal Syndrome Type I through Regulating the Inflammatory and Oxidative Stress Signaling

DOI: 10.1248/bpb.b17-00930
PMID: 30068867	Sample: Plasma

Biological and Pharmaceutical Bulletin (2018) IF: 1.6941

Qiliqiangxin Protects against Renal Injury in Rat with Cardiorenal Syndrome Type I through Regulating the Inflammatory and Oxidative Stress Signaling.

DOI: 10.1248/bpb.b17-00930
PMID: 30068867	Sample: Plasma

Experimental and Therapeutic Medicine IF: 1.41

Effect of parathyroid hormone on cardiac function in rats with cardiomyopathy.

DOI: 10.3892/etm.2018.6528
PMID: 30214507	Sample: Serum

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