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Rat BNP(Brain Natriuretic Peptide) ELISA Kit

  • Cat.No.:E-EL-R0126

  • Reactivity: Rat

To Purchase E-EL-R0126

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Competitive-ELISA
Format 96T/48T
Assay time 2.0h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 50μL
Specificity This kit recognizes BNP in samples. No significant cross-reactivity or interference between BNP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of BNP concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with BNP. During the reaction, BNP in the sample or standard competes with a fixed amount of BNP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to BNP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of BNP in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level BNP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level BNP were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 96.20 313.10 672.80 97.20 338.80 634.50
Standard deviation 5.90 14.70 32.30 6.60 17.30 29.20
CV (%) 6.13 4.69 4.80 6.79 5.11 4.60

Recovery

The recovery of BNP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-107 101
EDTA plasma(n=8) 84-97 91
Cell culture media(n=8) 86-98 92

Linearity

Samples were spiked with high concentrations of Rat BNP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsNPPB, GC-B, B-Type Natriuretic Peptide, Natriuretic Peptide Precursor B
Research AreaCardiovascular

Assay Procedures

elisa assay procedure 1

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C

elisa assay procedure 2

2. Aspirate and wash the plate for 3 times

elisa assay procedure 3

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 4

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 5

5. Add 50μL Stop Solution

elisa assay procedure 6

6. Read the plate at 450nm immediately. Calculation of the results

Related Products

Elabscience® provides ancillary reagents required for ELISA kit developments.11 reagents in total, including reagents for coating, blocking, washing, improving the stability and reducing matrix interference etc.
Cat.No. Product Name Specifications
E-ELIR-001 ELISA Plate Coating Buffer(1×) 100mL, 1000mL
E-ELIR-002 ELISA Plate Coating Buffer(5×) 100mL, 1000mL
E-ELIR-003 ELISA Plate Blocking Buffer 100mL, 1000mL
E-ELIR-004 Wash Buffer for Sandwich-ELISA(25×) 100mL, 1000mL
E-ELIR-005 Wash Buffer for Competitive-ELISA(25×) 100mL, 1000mL
E-ELIR-006 Stop Solution 100mL, 1000mL
E-ELIR-007 HRP-conjugate Stabilizer 10mL, 100mL, 500mL
E-ELIR-008 HRP-conjugate Diluent 100mL, 1000mL
E-ELIR-009 Biotinylated Antibody Stabilizer 10mL, 100mL, 500mL
E-ELIR-0010 Biotinylated Antibody Diluent 10mL, 100mL, 500mL
E-ELIR-0011 Sample Diluent 10mL, 100mL, 500mL

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Cellular Physiology and Biochemistry (2018) IF: 5.104
    Renal Denervation Effects on Myocardial Fibrosis and Ventricular Arrhythmias in Rats with Ischemic Cardiomyopathy.

    DOI: 10.1159/000489653

    PMID: 29742489

  2. Cellular Physiology and Biochemistry (2018) IF: 5.5
    Renal Denervation Effects on Myocardial Fibrosis and Ventricular Arrhythmias in Rats with Ischemic Cardiomyopathy.

    DOI: 10.1159/000489653

    PMID: 29742489

    Sample: Plasma
  3. Frontiers in Pharmacology (2020) IF: 4.225
    Perindopril Improves Cardiac Function by Enhancing the Expression of SIRT3 and PGC-1α in a Rat Model of Isoproterenol-Induced Cardiomyopathy

    DOI: 10.3389/fphar.2020.00094

    PMID: 32153406

    Sample: Serum
  4. Biomedicine & Pharmacotherapy (2019) IF: 3.457
    The effects of Radix Angelica Sinensis and Radix Hedysari ultrafiltration extract on X-irradiation-induced myocardial fibrosis in rats

    DOI: 10.1016/j.biopha.2019.01.057

    PMID: 30780109

    Sample: Serum
  5. Biochemical and biophysical research communications (2019) IF: 2.985
    Therapeutic effects of erythropoietin expressed in mesenchymal stem cells for dilated cardiomyopathy in rat

    DOI: 10.1016/j.bbrc.2019.07.053

    PMID: 31400858

    Sample: Serum,Tissue homogenate
  6. Acta biochimica et biophysica Sinica (2018) IF: 2.224
    AdipoRon prevents l-thyroxine or isoproterenol-induced cardiac hypertrophy through regulating the AMPK-related pathway

    DOI: 10.1093/abbs/gmy152

    PMID: 30566571

    Sample: Serum
  7. Experimental and therapeutic medicine (2018) IF: 1.41
    Effect of parathyroid hormone on cardiac function in rats with cardiomyopathy

    DOI: 10.3892/etm.2018.6528

    PMID: 30214507

    Sample: Serum
  8. Biological and Pharmaceutical Bulletin (2018) IF: 1.54
    Qiliqiangxin Protects against Renal Injury in Rat with Cardiorenal Syndrome Type I through Regulating the Inflammatory and Oxidative Stress Signaling

    DOI: 10.1248/bpb.b17-00930

    PMID: 30068867

    Sample: Plasma
  9. Biological and Pharmaceutical Bulletin (2018) IF: 1.6941
    Qiliqiangxin Protects against Renal Injury in Rat with Cardiorenal Syndrome Type I through Regulating the Inflammatory and Oxidative Stress Signaling.

    DOI: 10.1248/bpb.b17-00930

    PMID: 30068867

    Sample: Plasma
  10. Experimental and Therapeutic Medicine IF: 1.41
    Effect of parathyroid hormone on cardiac function in rats with cardiomyopathy.

    DOI: 10.3892/etm.2018.6528

    PMID: 30214507

    Sample: Serum
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