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Rat CASP3(Caspase 3) ELISA Kit

  • Cat.No.:E-EL-R0160

  • Reactivity: Rat

To Purchase E-EL-R0160

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.31-20 ng/mL
Sensitivity 0.19 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat CASP3 in samples. No significant cross-reactivity or interference between Rat CASP3 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat CASP3 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CASP3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CASP3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CASP3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CASP3. You can calculate the concentration of Rat CASP3 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat CASP3 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat CASP3 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 1.09 3.21 9.83 1.05 3.10 9.92
Standard deviation 0.07 0.14 0.54 0.06 0.12 0.49
CV (%) 6.42 4.36 5.49 5.71 3.87 4.94

Recovery

The recovery of Rat CASP3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 96-107 101
EDTA plasma (n=8) 87-102 93
Cell culture media (n=8) 91-101 96

Linearity

Samples were spiked with high concentrations of Rat CASP3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: P55213
SynonymsCPP32, CPP32B, SCA-1, Apoptain, Yama
Research AreaCancer,Cell biology,Metabolism,Neuroscience

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Related Products

Elabscience® provides ancillary reagents required for ELISA kit developments.11 reagents in total, including reagents for coating, blocking, washing, improving the stability and reducing matrix interference etc.
Cat.No. Product Name Specifications
E-ELIR-001 ELISA Plate Coating Buffer(1×) 100mL, 1000mL
E-ELIR-002 ELISA Plate Coating Buffer(5×) 100mL, 1000mL
E-ELIR-003 ELISA Plate Blocking Buffer 100mL, 1000mL
E-ELIR-004 Wash Buffer for Sandwich-ELISA(25×) 100mL, 1000mL
E-ELIR-005 Wash Buffer for Competitive-ELISA(25×) 100mL, 1000mL
E-ELIR-006 Stop Solution 100mL, 1000mL
E-ELIR-007 HRP-conjugate Stabilizer 10mL, 100mL, 500mL
E-ELIR-008 HRP-conjugate Diluent 100mL, 1000mL
E-ELIR-009 Biotinylated Antibody Stabilizer 10mL, 100mL, 500mL
E-ELIR-0010 Biotinylated Antibody Diluent 10mL, 100mL, 500mL
E-ELIR-0011 Sample Diluent 10mL, 100mL, 500mL

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

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    Selenium abates reproductive dysfunction via attenuation of biometal accumulation, oxido-inflammatory stress and caspase-3 activation in male rats exposed to arsenic

    DOI: 10.1016/j.envpol.2019.113079

    PMID: 31473390

    Sample: Serum
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    Impact of binary waterborne mixtures of nickel and zinc on hypothalamic-pituitary-testicular axis in rats

    DOI: 10.1016/j.chemosphere.2019.124501

    PMID: 31398612

    Sample: Serum
  3. Toxicology (2020) IF: 4.099
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    DOI: 10.1016/j.tox.2020.152556

    PMID: 32758512

    Sample: Serum,Tissue homogenate
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    DOI: 10.1002/ptr.6827

    PMID: 32785956

    Sample: Cell lysate
  5. Biomedicine & Pharmacotherapy (2018) IF: 4.545
    The effects of safranal, a constitute of saffron, and metformin on spatial learning and memory impairments in type-1 diabetic rats: behavioral and hippocampal histopathological and biochemical evaluations

    DOI: 10.1016/j.biopha.2018.07.165

    PMID: 30092399

    Sample: Tissue homogenate
  6. Journal of King Saud University-Science (2020) IF: 3.819
    The protecting role of Moringa oleifera in cypermethrin-induced mitochondrial dysfunction and apoptotic events in rats brain

    DOI: 10.1016/j.jksus.2020.06.006

    PMID: 99999999

    Sample: Tissue homogenate
  7. International Immunopharmacology (2020) IF: 3.943
    The ameliorating effect of cannabinoid type 2 receptor activation on brain, lung, liver and heart damage in cecal ligation and puncture-induced sepsis model in rats

    DOI: 10.1016/j.intimp.2019.105978

    PMID: 31767546

    Sample: Tissue homogenate
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    Neuroprotective mechanisms of selenium against arsenic-induced behavioral impairments in rats

    DOI: 10.1016/j.neuro.2019.10.009

    PMID: 31678059

    Sample: Tissue homogenate
  9. Food and Chemical Toxicology (2019) IF: 3.977
    Protocatechuic acid ameliorates neurobehavioral deficits via suppression of oxidative damage, inflammation, caspase-3 and acetylcholinesterase activities in diabetic rats

    DOI: 10.1016/j.fct.2018.12.040

    PMID: 30597223

    Sample: Tissue homogenate
  10. Life sciences (2019) IF: 3.234
    Safranal, a constituent of saffron, exerts gastro-protective effects against indomethacin-induced gastric ulcer

    DOI: 10.1016/j.lfs.2019.03.054

    PMID: 30914317

    Sample: Tissue homogenate
  11. European journal of pharmacology (2019) IF: 3.04
    Dietary protocatechuic acid abrogates male reproductive dysfunction in streptozotocin-induced diabetic rats via suppression of oxidative damage, inflammation and caspase-3 activity

    DOI: 10.1016/j.ejphar.2019.01.033

    PMID: 30690002

    Sample: Tissue homogenate
  12. Chemico-Biological Interactions (2017) IF: 3.143
    Protective Properties Of 6-Gingerol-Rich Fraction From Zingiber Officinale (Ginger) On Chlorpyrifos-Induced Oxidative Damage And Inflammation In The Brain, Ovary And Uterus Of Rats.

    DOI: 10.1016/j.cbi.2017.03.017

    PMID: 28373059

    Sample: Tissue homogenate
  13. Chemico-Biological Interactions (2017) IF: 3.143
    Chemoprotective Role Of Quercetin In Manganese-Induced Toxicity Along The Brain-Pituitary-Testicular Axis In Rats.

    DOI: 10.1016/j.cbi.2016.12.019

    PMID: 28040552

    Sample: Tissue homogenate
  14. Drug and Chemical Toxicology (2019) IF: 2.405
    Behavioral, histopathological, and biochemical evaluations on the effects of cinnamaldehyde, naloxone, and their combination in morphine-induced cerebellar toxicity

    DOI: 10.1080/01480545.2019.1681446

    PMID: 31656103

    Sample: Tissue homogenate
  15. Neuroscience Letters (2018) IF: 2.159
    Rutin Attenuates Neurobehavioral Deficits, Oxidative Stress, Neuro-Inflammation and Apoptosis in Fluoride Treated Rats.

    DOI: 10.1016/j.neulet.2018.06.023

    PMID: 29908257

    Sample: Tissue homogenate
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    Sirt1 Exerts Neuroprotective Effects By Attenuating Cerebral Ischemia/Reperfusion-Induced Injury Via Targeting P53/Microrna-22.

    DOI: 10.3892/ijmm.2016.2806

    PMID: 27878231

    Sample: Tissue homogenate
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    Fluoride and diethylnitrosamine coexposure enhances oxido-inflammatory responses and caspase-3 activation in liver and kidney of adult rats

    DOI: 10.1002/jbt.22327

    PMID: 30920066

    Sample: Serum
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    Taurine Reverses Sodium Fluoride-Mediated Increase In Inflammation, Caspase-3 Activity And Oxidative Damage Along The Brain-Pituitary-Gonadal Axis In Male Rats.

    DOI: 10.1139/cjpp-2016-0641

    PMID: 28654759

    Sample: Tissue homogenate
  19. Molecular Medicine Reports (2016) IF: 1.692
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    DOI: 10.3892/mmr.2015.4615

    PMID: 26648531

    Sample: Tissue homogenate
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    DOI: 10.1183/23120541.00174-2020

    PMID: 33043050

    Sample: Tissue homogenate
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    Myricetin Ameliorates Brain Damage Induces by Cerebral Ischemia-Reperfusion Injury in Rats

    DOI: 10.22377/ajp.v14i1.3474

    PMID: 99999999

    Sample: Tissue homogenate
  22. Turkish Journal of Vascular Surgery (2019)
    Effects of mesenchymal stem cells to prevent adhesions for vascular reoperations: An experimental study

    DOI: 10.9739/tjvs.2019.560

    PMID: 99999999

    Sample: Serum,Plasma,Tissue homogenate
  23. Journal of Herbs, Spices & Medicinal Plants (2020)
    Modulatory Effects of Ethyl Acetate and Methanol Fractions of the Stem Bark Extract of Alstonia boonei on Mitochondrial-Mediated Apoptosis

    DOI: 10.1080/10496475.2020.1747582

    PMID: 99999999

    Sample: Tissue homogenate
  24. Asian Journal of Pharmaceutical and Clininical Research (2018)
    EFFECTS OF VITAMIN E AND COENZYME Q10 SUPPLEMENTATION AGAINST DOXORUBICIN-INDUCED NEUROTOXICITY IN RATS: ROLE OF APOPTOSIS

    DOI: 99999999

    PMID: 99999999

    Sample: Tissue homogenate
  25. Pathophysiology (2019)
    Coconut oil protects against light-induced retina degeneration in male Wistar rats

    DOI: 10.1016/j.pathophys.2018.10.001

    PMID: 30309759

    Sample: Tissue homogenate
  26. Journal of?HerbMed?Pharmacology (2018)
    Methanol fraction of Calliandra portoricensis root bark activates caspases via alteration in mitochondrial viability in vivo

    DOI: 10.15171/jhp.2018.38

    PMID: 99999999

    Sample: Tissue homogenate
  27. International Journal of Pharmaceutical Sciences Review and Research (2017)
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    DOI: 99999999

    PMID: 99999999

    Sample: Tissue homogenate
  28. International Journal of Pharmaceutical Sciences Review and Research (2017)
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    DOI: 99999999

    PMID: 99999999

    Sample: Tissue homogenate
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    Manganese suppresses oxidative stress, inflammation and caspase-3 activation in rats exposed to chlorpyrifos

    DOI: 10.1016/j.toxrep.2019.02.007

    PMID: 30859069

    Sample: Tissue homogenate
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