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Rat CASP9(Caspase 9) ELISA Kit

Cat.No.:E-EL-R0163
Reactivity: Rat

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96T
48T
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96T*5
96T*10

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	1.56-100 ng/mL
Sensitivity	0.94 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat CASP9 in samples. No significant cross-reactivity or interference between Rat CASP9 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat CASP9 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CASP9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CASP9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CASP9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CASP9. You can calculate the concentration of Rat CASP9 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat CASP9 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat CASP9 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	4.89	12.56	37.09	4.75	13.80	37.03
Standard deviation	0.30	0.54	1.86	0.25	0.62	1.31
CV (%)	6.13	4.30	5.01	5.26	4.49	3.54

Recovery

The recovery of Rat CASP9 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	90-104	97
EDTA plasma (n=8)	93-105	99
Cell culture media (n=8)	85-99	91

Linearity

Samples were spiked with high concentrations of Rat CASP9 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Research Area

Cancer, Cell Biology, Metabolism

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

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Citations

LIFE SCIENCES (2023) IF: 6.78
The GSTP1/MAPKs/BIM/SMAC modulatory actions of nitazoxanide: Bioinformatics and experimental evidence in subcutaneous solid Ehrlich carcinoma-inoculated mice

DOI: 10.1016/j.lfs.2023.121496

Sample: Ehrlich tumour tissues
CHEMICO-BIOLOGICAL INTERACTIONS (2021) IF: 5.194
Plumbagin induces testicular damage via mitochondrial-dependent cell death

DOI: 10.1016/j.cbi.2021.109582

PMID: 34302802
Sample: testis
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY (2022) IF: 5.785
Influence of furan and lead co-exposure at environmentally relevant concentrations on neurobehavioral performance, redox-regulatory system and apoptotic responses in rats

DOI: 10.1016/j.etap.2022.104011

Sample: cerebellum,cerebrum
Frontiers in Pharmacology (2021) IF: 5.811
Therapeutic Potentials of Selected Antihypertensive Agents and Their Fixed-Dose Combinations Against Trastuzumab-Mediated Cardiotoxicity

DOI: 10.3389/fphar.2020.610331

Sample: Cardiac tissue
Mycotoxin Research (2023) IF: 4.082
Neurobehavioral and biochemical responses to artemisinin-based drug and aflatoxin B1 co-exposure in rats

DOI: 10.1007/s12550-023-00474-6

Sample: cerebellar,cerebral
TOXICOLOGY (2020) IF: 4.099
Protocatechuic acid modulates reproductive dysfunction linked to furan exposure in rats

DOI: 10.1016/j.tox.2020.152556

PMID: 32758512
Sample: Serum,Tissue homogenate
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2022) IF: 4.223
3-Indolepropionic acid prevented chlorpyrifos-induced hepatorenal toxicities in rats by improving anti-inflammatory, antioxidant, and pro-apoptotic responses and abating DNA damage

DOI: 10.1007/s11356-022-21075-3

PMID: 35644820
Sample: Hepatic,renal
JOURNAL OF PHARMACY AND PHARMACOLOGY (2022) IF: 4.81
Bromelain ameliorates D-galactosamine-induced acute liver injury: role of SIRT1/LKB1/AMPK, GSK3β/Nrf2 and NF-κB p65/TNF-α/caspase-8, -9 signalling pathways

DOI: 10.1093/jpp/rgac071

PMID: 36227279
Sample: hepatic contents
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY (2021) IF: 4.86
Indole-3-propionic acid mitigates chlorpyrifos-mediated neurotoxicity by modulating cholinergic and redox-regulatory systems, inflammatory stress, apoptotic responses and DNA damage in rats

DOI: 10.1016/j.etap.2021.103786

PMID: 34915193
Sample: brain
TOXICON (2022) IF: 3.033
Caffeic acid protects against DNA damage, oxidative and inflammatory mediated toxicities, and upregulated caspases activation in the hepatorenal system of rats treated with aflatoxin B1

DOI: 10.1016/j.toxicon.2021.12.021

PMID: 34995555
Sample: liver,kidney
TOXICON (2022) IF: 3.035
Co-exposure to aflatoxin B1 and therapeutic coartem worsens hepatic and renal function through enhanced oxido-inflammatory responses and apoptosis in rats

DOI: 10.1016/j.toxicon.2022.106988

Sample: liver，kidney
Toxicology Research (2021) IF: 3.524
Chlorogenic acid abates oxido-inflammatory and apoptotic responses in the liver and kidney of Tamoxifen-treated rats

DOI: 10.1093/toxres/tfab002

Sample: hepatic,renal
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2021) IF: 3.652
Biochemical and histological alterations of doxorubicin-induced neurotoxicity in rats: Protective role of luteolin

DOI: 10.1002/jbt.22962

PMID: 34766659
Sample: brain
TOXICOLOGY MECHANISMS AND METHODS (2020) IF: 2.295
Comparative effects of galactose-induced aging on mitochondrial permeability transition in rat liver and testis

DOI: 10.1080/15376516.2020.1755921

Sample: liver
BMC Pharmacology & Toxicology (2022) IF: 2.605
Accidental lead in contaminated pipe-borne water and dietary furan intake perturbs rats’ hepatorenal function altering oxidative, inflammatory, and apoptotic balance

DOI: 10.1186/s40360-022-00615-0

Sample: hepatic,renal
FUNDAMENTAL & CLINICAL PHARMACOLOGY (2022) IF: 2.747
The protective effect of 3-indolepropanoic acid on aflatoxin B1-induced systemic perturbation of the liver and kidney function in rats

DOI: 10.1111/fcp.12842

PMID: 36214208
Sample: liver,kidney
HUMAN & EXPERIMENTAL TOXICOLOGY (2021) IF: 2.903
Luteolin attenuates doxorubicin-induced derangements of liver and kidney by reducing oxidative and inflammatory stress to suppress apoptosis:

DOI: 10.1177/09603271211006171

Sample: Tissue homogenate
HUMAN & EXPERIMENTAL TOXICOLOGY (2021) IF: 2.903
Attenuation of diethyl nitrosamine-induced hepatocellular carcinoma by taxifolin and/or alogliptin: The interplay between toll-like receptor 4, transforming growth factor beta-1, and apoptosis:

DOI: 10.1177/09603271211008496

Sample: Tissue Slice,Serum
Molecular Medicine Reports (2016) IF: 2.952
Astaxanthin reduces type 2 diabetic‑associated cognitive decline in rats via activation of PI3K/Akt and attenuation of oxidative stress

DOI: 10.3892/mmr.2015.4615

Sample: Tissue homogenate

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