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Rat CGRP1(Calcitonin Gene Related Peptide 1) ELISA Kit

Cat.No.:E-EL-R0135
Reactivity: Rat

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96T
48T
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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	15.63-1000 pg/mL
Sensitivity	9.38 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat CGRP1 in samples. No significant cross-reactivity or interference between Rat CGRP1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat CGRP1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CGRP1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CGRP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CGRP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CGRP1. You can calculate the concentration of Rat CGRP1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat CGRP1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat CGRP1 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	47.62	96.89	346.87	49.73	105.67	368.64
Standard deviation	2.52	4.83	18.42	2.60	4.87	19.28
CV (%)	5.29	4.99	5.31	5.23	4.61	5.23

Recovery

The recovery of Rat CGRP1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	91-104	98
EDTA plasma (n=8)	90-106	97
Cell culture media (n=8)	95-108	101

Linearity

Samples were spiked with high concentrations of Rat CGRP1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	CGRP1
Research Area	Metabolism, Signal transduction, Cancer

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Cell Metabolism (2022) IF: 31.373
Cancer cells co-opt nociceptive nerves to thrive in nutrient-poor environments and upon nutrient-starvation therapies

DOI: 10.1016/j.cmet.2022.10.012

PMID: 36395769
Sample: plasma
NATURE MEDICINE (2016) IF: 30.357
Implant-derived magnesium induces local neuronal production of CGRP to improve bone-fracture healing in rats

DOI: 10.1038/nm.4162

PMID: 27571347
Sample: Tissue homogenate
PHYTOMEDICINE (2021) IF: 5.34
Extracts of Poria cocos improve functional dyspepsia via regulating brain-gut peptides, immunity and repairing of gastrointestinal mucosa

DOI: 10.1016/j.phymed.2021.153875

PMID: 34911003
Sample: serum
Aging-US (2020) IF: 4.831
NO up-regulates migraine-related CGRP via activation of an Akt/GSK-3β/NF-κB signaling cascade in trigeminal ganglion neurons

DOI: 10.18632/aging.103031

Sample: Primary TGNs
EUROPEAN JOURNAL OF NEUROSCIENCE (2020) IF: 3.115
Receptor mechanisms mediating the anti-neuroinflammatory effects of endocannabinoid system modulation in a rat model of migraine

DOI: 10.1111/ejn.14897

Sample: plasma,trigeminal ganglia,brainstem
ENDOCRINE (2018) IF: 3.179
Serum calcitonin gene-related peptide facilitates adipose tissue lipolysis during exercise via PIPLC/IP3 pathways

DOI: 10.1007/s12020-018-1640-2

Sample: Serum
CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY (2018) IF: 2.092
Salmon calcitonin ameliorates migraine pain through modulation of CGRP release and dural mast cell degranulation in rats

DOI: 10.1111/1440-1681.12915

Sample: plasma,trigeminal ganglions,trigeminal ganglion ne
International Journal of Medical Sciences (2018) IF: 2.284
Pulsed Radiofrequency Applied to the Sciatic Nerve Improves Neuropathic Pain by Down-regulating The Expression of Calcitonin Gene-related Peptide in the Dorsal Root Ganglion

DOI: 10.7150/ijms.20501

Sample: Tissue homogenate
BRAIN RESEARCH (2019) IF: 2.929
Cervical sympathectomy modulates the neurogenic inflammatory neuropeptides following experimental subarachnoid hemorrhage in rats

DOI: 10.1016/j.brainres.2019.146366

PMID: 31401069
Sample: Plasma,Tissue homogenate
INFLAMMATION (2019) IF: 2.939
Thymoquinone Inhibits Neurogenic Inflammation Underlying Migraine Through Modulation of Calcitonin Gene-Related Peptide Release and Stabilization of Meningeal Mast Cells in Glyceryltrinitrate-Induced Migraine Model in Rats

DOI: 10.1007/s10753-019-01115-w

PMID: 31707574
Sample: Whole Blood
Chinese Medicine (2020) IF: 2.96
Hejie Zhitong prescription promotes sleep and inhibits nociceptive transmission-associated neurotransmitter activity in a rodent migraine model

DOI: 10.1186/s13020-020-00386-y

PMID: 33014123
Sample: Whole Blood
Experimental and Therapeutic Medicine (2019) IF: 1.448
Fructus Viticis methanolic extract attenuates trigeminal hyperalgesia in migraine by regulating injury signal transmission

DOI: 10.3892/etm.2019.8201

Sample: blood
Revista da Associacao Medica Brasileira (2019) IF: 0.801
Modulatory effects of neuropeptides on pentylenetetrazol-induced epileptic seizures and neuroinflammation in rats

DOI: 10.1590/1806-9282.65.9.1188

PMID: 31618336
Sample: Plasma

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