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Rat CGRP1(Calcitonin Gene Related Peptide 1) ELISA Kit

  • Cat.No.:E-EL-R0135

  • Reactivity: Rat

To Purchase E-EL-R0135

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat CGRP1 in samples. No significant cross-reactivity or interference between Rat CGRP1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat CGRP1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CGRP1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CGRP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CGRP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CGRP1. You can calculate the concentration of Rat CGRP1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat CGRP1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat CGRP1 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 47.62 96.89 346.87 49.73 105.67 368.64
Standard deviation 2.52 4.83 18.42 2.60 4.87 19.28
CV (%) 5.29 4.99 5.31 5.23 4.61 5.23


The recovery of Rat CGRP1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-104 98
EDTA plasma (n=8) 90-106 97
Cell culture media (n=8) 95-108 101


Samples were spiked with high concentrations of Rat CGRP1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Research AreaMetabolism, Signal transduction, Cancer

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Cell Metabolism (2022) IF: 31.373
    Cancer cells co-opt nociceptive nerves to thrive in nutrient-poor environments and upon nutrient-starvation therapies

    DOI: 10.1016/j.cmet.2022.10.012

    PMID: 36395769

    Sample: plasma
  2. NATURE MEDICINE (2016) IF: 30.357
    Implant-derived magnesium induces local neuronal production of CGRP to improve bone-fracture healing in rats

    DOI: 10.1038/nm.4162

    PMID: 27571347

    Sample: Tissue homogenate
  3. PHYTOMEDICINE (2021) IF: 5.34
    Extracts of Poria cocos improve functional dyspepsia via regulating brain-gut peptides, immunity and repairing of gastrointestinal mucosa

    DOI: 10.1016/j.phymed.2021.153875

    PMID: 34911003

    Sample: serum
  4. Aging-US (2020) IF: 4.831
    NO up-regulates migraine-related CGRP via activation of an Akt/GSK-3β/NF-κB signaling cascade in trigeminal ganglion neurons

    DOI: 10.18632/aging.103031

    Sample: Primary TGNs
    Receptor mechanisms mediating the anti-neuroinflammatory effects of endocannabinoid system modulation in a rat model of migraine

    DOI: 10.1111/ejn.14897

    Sample: plasma,trigeminal ganglia,brainstem
  6. ENDOCRINE (2018) IF: 3.179
    Serum calcitonin gene-related peptide facilitates adipose tissue lipolysis during exercise via PIPLC/IP3 pathways

    DOI: 10.1007/s12020-018-1640-2

    Sample: Serum
    Salmon calcitonin ameliorates migraine pain through modulation of CGRP release and dural mast cell degranulation in rats

    DOI: 10.1111/1440-1681.12915

    Sample: plasma,trigeminal ganglions,trigeminal ganglion ne
  8. International Journal of Medical Sciences (2018) IF: 2.284
    Pulsed Radiofrequency Applied to the Sciatic Nerve Improves Neuropathic Pain by Down-regulating The Expression of Calcitonin Gene-related Peptide in the Dorsal Root Ganglion

    DOI: 10.7150/ijms.20501

    Sample: Tissue homogenate
  9. BRAIN RESEARCH (2019) IF: 2.929
    Cervical sympathectomy modulates the neurogenic inflammatory neuropeptides following experimental subarachnoid hemorrhage in rats

    DOI: 10.1016/j.brainres.2019.146366

    PMID: 31401069

    Sample: Plasma,Tissue homogenate
  10. INFLAMMATION (2019) IF: 2.939
    Thymoquinone Inhibits Neurogenic Inflammation Underlying Migraine Through Modulation of Calcitonin Gene-Related Peptide Release and Stabilization of Meningeal Mast Cells in Glyceryltrinitrate-Induced Migraine Model in Rats

    DOI: 10.1007/s10753-019-01115-w

    PMID: 31707574

    Sample: Whole Blood
  11. Chinese Medicine (2020) IF: 2.96
    Hejie Zhitong prescription promotes sleep and inhibits nociceptive transmission-associated neurotransmitter activity in a rodent migraine model

    DOI: 10.1186/s13020-020-00386-y

    PMID: 33014123

    Sample: Whole Blood
  12. Experimental and Therapeutic Medicine (2019) IF: 1.448
    Fructus Viticis methanolic extract attenuates trigeminal hyperalgesia in migraine by regulating injury signal transmission

    DOI: 10.3892/etm.2019.8201

    Sample: blood
  13. Revista da Associacao Medica Brasileira (2019) IF: 0.801
    Modulatory effects of neuropeptides on pentylenetetrazol-induced epileptic seizures and neuroinflammation in rats

    DOI: 10.1590/1806-9282.65.9.1188

    PMID: 31618336

    Sample: Plasma
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